Isolation and selection of Bacillus cereus specific phages from hospital wastewater

Abstract. Rovik A, ‘Aziz S, Pramono H. 2020. Isolation and selection of Bacillus cereus specific phages from hospital wastewater. Biodiversitas 21: 2871-2877. Bacillus cereus (B. cereus) is a pathogenic bacterium that frequently contaminates food by producing entero and emetic toxins. B. cereus has...

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Autores principales:	Anwar Rovik, SAEFUDDIN ‘AZIZ, HENDRO PRAMONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacillus cereus, bacteriophage, banyumas, hospital wastewater, specific Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c7697461e02740c9a545cab95d0f41ca
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spelling	oai:doaj.org-article:c7697461e02740c9a545cab95d0f41ca2021-11-22T00:40:52ZIsolation and selection of Bacillus cereus specific phages from hospital wastewater1412-033X2085-472210.13057/biodiv/d210701https://doaj.org/article/c7697461e02740c9a545cab95d0f41ca2020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5922https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Rovik A, ‘Aziz S, Pramono H. 2020. Isolation and selection of Bacillus cereus specific phages from hospital wastewater. Biodiversitas 21: 2871-2877. Bacillus cereus (B. cereus) is a pathogenic bacterium that frequently contaminates food by producing entero and emetic toxins. B. cereus has shown resistance to various antibiotics, especially ?-lactam antibiotics. An alternative to control B. cereus contamination is the use of bacteriophages. This study aimed to isolate and screen B. cereus specific phages from hospital wastewater in Banyumas District. The research was conducted descriptively through isolation, purification, titer determination, host ranges, and adsorption rate determination. A total of 29 isolates of B. cereus-phages were isolated from hospital wastewater in Banyumas with various titers, ranged from 0.14-3.76 x 107 PFU.mL-1. Isolated phages could be grouped into two, narrow host range (14 phages) and broad activity spectra (15 phages) that infect both Gram-positive and negative bacteria i.e. B. subtilis, B. fragilis, B. licheniformis, Citrobacter freundii, Klebsiella pneumoniae, Salmonella thypi, and Escherichia coli. The infections had a latency period of 60-120 minutes. The decrease of culture absorbance value ranged from 0.06 to 0.41, while the control tended to increase by 0.39. The largest decreases were showed by phage isolates RSBMS-2 and RSBMT-1 with 0.41 and 0.37, respectively.Anwar RovikSAEFUDDIN ‘AZIZHENDRO PRAMONOMBI & UNS Soloarticlebacillus cereus, bacteriophage, banyumas, hospital wastewater, specificBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus cereus, bacteriophage, banyumas, hospital wastewater, specific Biology (General) QH301-705.5
spellingShingle	bacillus cereus, bacteriophage, banyumas, hospital wastewater, specific Biology (General) QH301-705.5 Anwar Rovik SAEFUDDIN ‘AZIZ HENDRO PRAMONO Isolation and selection of Bacillus cereus specific phages from hospital wastewater
description	Abstract. Rovik A, ‘Aziz S, Pramono H. 2020. Isolation and selection of Bacillus cereus specific phages from hospital wastewater. Biodiversitas 21: 2871-2877. Bacillus cereus (B. cereus) is a pathogenic bacterium that frequently contaminates food by producing entero and emetic toxins. B. cereus has shown resistance to various antibiotics, especially ?-lactam antibiotics. An alternative to control B. cereus contamination is the use of bacteriophages. This study aimed to isolate and screen B. cereus specific phages from hospital wastewater in Banyumas District. The research was conducted descriptively through isolation, purification, titer determination, host ranges, and adsorption rate determination. A total of 29 isolates of B. cereus-phages were isolated from hospital wastewater in Banyumas with various titers, ranged from 0.14-3.76 x 107 PFU.mL-1. Isolated phages could be grouped into two, narrow host range (14 phages) and broad activity spectra (15 phages) that infect both Gram-positive and negative bacteria i.e. B. subtilis, B. fragilis, B. licheniformis, Citrobacter freundii, Klebsiella pneumoniae, Salmonella thypi, and Escherichia coli. The infections had a latency period of 60-120 minutes. The decrease of culture absorbance value ranged from 0.06 to 0.41, while the control tended to increase by 0.39. The largest decreases were showed by phage isolates RSBMS-2 and RSBMT-1 with 0.41 and 0.37, respectively.
format	article
author	Anwar Rovik SAEFUDDIN ‘AZIZ HENDRO PRAMONO
author_facet	Anwar Rovik SAEFUDDIN ‘AZIZ HENDRO PRAMONO
author_sort	Anwar Rovik
title	Isolation and selection of Bacillus cereus specific phages from hospital wastewater
title_short	Isolation and selection of Bacillus cereus specific phages from hospital wastewater
title_full	Isolation and selection of Bacillus cereus specific phages from hospital wastewater
title_fullStr	Isolation and selection of Bacillus cereus specific phages from hospital wastewater
title_full_unstemmed	Isolation and selection of Bacillus cereus specific phages from hospital wastewater
title_sort	isolation and selection of bacillus cereus specific phages from hospital wastewater
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/c7697461e02740c9a545cab95d0f41ca
work_keys_str_mv	AT anwarrovik isolationandselectionofbacilluscereusspecificphagesfromhospitalwastewater AT saefuddinaziz isolationandselectionofbacilluscereusspecificphagesfromhospitalwastewater AT hendropramono isolationandselectionofbacilluscereusspecificphagesfromhospitalwastewater
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Isolation and selection of Bacillus cereus specific phages from hospital wastewater

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