EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES

Abstract. An intracellular staining technique of marker cytokines was applied for evaluation of quantitative distribution of T-lymphocyte effector subsets in children and adults. The results of this study have shown that the numbers of Th2 lymphocytes in infants (7 to 12 months old) correspond to ap...

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Autores principales:	Yu. G. Lagereva, S. V. Menshikov, T. L. Savinova, J. B. Beykin, V. A. Chereshnev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	т-cell effectors/memory cells flow cytometry children adults Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c773f062605a45479335a4af8902cec3
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spelling	oai:doaj.org-article:c773f062605a45479335a4af8902cec32021-11-18T08:03:41ZEVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES1563-06252313-741X10.15789/1563-0625-2012-4-5-295-304https://doaj.org/article/c773f062605a45479335a4af8902cec32014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/461https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. An intracellular staining technique of marker cytokines was applied for evaluation of quantitative distribution of T-lymphocyte effector subsets in children and adults. The results of this study have shown that the numbers of Th2 lymphocytes in infants (7 to 12 months old) correspond to appropriate normal values in adults, being accompanied by decreased Th1 and Тс1cell contents, whereas absolute amounts of Tc2 lymphocytes proved to be higher than in all other age groups. Absolute counts of Th17 and Tnc17 subsets in infants of 7 to 12 months were higher than in adults. Hence, these infants exhibited a shift of immune response towards Th2 (Tc2) and Th17 (Tnc17), thus presenting a potential factor of Th2-mediated allergic disorders in the children under one year. In children over 1 year, absolute amounts of Th1 and Тс1, as well as Th2 and Тс2 lymphocyte counts did not significantly differ from adult values. Th2 lymphocyte counts among adolescents (15 to 18 years) were decreased, along with relative increase of IL-17А-positive lymphocyte scores, thus reflecting a probable predisposition for autoimmune disorders induced at this age. The age-dependent increase in Th1 and Тс1 counts in children may immediately correlate with formation of T-cell memory pool.Yu. G. LagerevaS. V. MenshikovT. L. SavinovaJ. B. BeykinV. A. ChereshnevSPb RAACIarticleт-cell effectors/memory cellsflow cytometrychildrenadultsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 4-5, Pp 295-304 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	т-cell effectors/memory cells flow cytometry children adults Immunologic diseases. Allergy RC581-607
spellingShingle	т-cell effectors/memory cells flow cytometry children adults Immunologic diseases. Allergy RC581-607 Yu. G. Lagereva S. V. Menshikov T. L. Savinova J. B. Beykin V. A. Chereshnev EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
description	Abstract. An intracellular staining technique of marker cytokines was applied for evaluation of quantitative distribution of T-lymphocyte effector subsets in children and adults. The results of this study have shown that the numbers of Th2 lymphocytes in infants (7 to 12 months old) correspond to appropriate normal values in adults, being accompanied by decreased Th1 and Тс1cell contents, whereas absolute amounts of Tc2 lymphocytes proved to be higher than in all other age groups. Absolute counts of Th17 and Tnc17 subsets in infants of 7 to 12 months were higher than in adults. Hence, these infants exhibited a shift of immune response towards Th2 (Tc2) and Th17 (Tnc17), thus presenting a potential factor of Th2-mediated allergic disorders in the children under one year. In children over 1 year, absolute amounts of Th1 and Тс1, as well as Th2 and Тс2 lymphocyte counts did not significantly differ from adult values. Th2 lymphocyte counts among adolescents (15 to 18 years) were decreased, along with relative increase of IL-17А-positive lymphocyte scores, thus reflecting a probable predisposition for autoimmune disorders induced at this age. The age-dependent increase in Th1 and Тс1 counts in children may immediately correlate with formation of T-cell memory pool.
format	article
author	Yu. G. Lagereva S. V. Menshikov T. L. Savinova J. B. Beykin V. A. Chereshnev
author_facet	Yu. G. Lagereva S. V. Menshikov T. L. Savinova J. B. Beykin V. A. Chereshnev
author_sort	Yu. G. Lagereva
title	EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
title_short	EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
title_full	EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
title_fullStr	EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
title_full_unstemmed	EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
title_sort	evaluation of t-cell effector subpopulations distribution in children and adults by means of intracellular staining of cytokines
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/c773f062605a45479335a4af8902cec3
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EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES

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