MicroRNA expression in human omental and subcutaneous adipose tissue.

MicroRNAs (miRNAs) are small non-coding RNAs, that play important regulatory roles in a variety of biological processes, including development, differentiation, apoptosis, and metabolism. In mammals, miRNAs have been shown to modulate adipocyte differentiation. Therefore, we performed a global miRNA...

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Autores principales:	Nora Klöting, Susan Berthold, Peter Kovacs, Michael R Schön, Mathias Fasshauer, Karen Ruschke, Michael Stumvoll, Matthias Blüher
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c778e0a6c5a04b689b62d6a759af6789
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spelling	oai:doaj.org-article:c778e0a6c5a04b689b62d6a759af67892021-11-25T06:16:57ZMicroRNA expression in human omental and subcutaneous adipose tissue.1932-620310.1371/journal.pone.0004699https://doaj.org/article/c778e0a6c5a04b689b62d6a759af67892009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19259271/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) are small non-coding RNAs, that play important regulatory roles in a variety of biological processes, including development, differentiation, apoptosis, and metabolism. In mammals, miRNAs have been shown to modulate adipocyte differentiation. Therefore, we performed a global miRNA gene expression assay in different fat depots of overweight and obese individuals to investigate whether miRNA expression in human adipose tissue is fat-depot specific and associated with parameters of obesity and glucose metabolism. Paired samples of abdominal subcutaneous (SC) and intraabdominal omental adipose tissue were obtained from fifteen individuals with either normal glucose tolerance (NGT, n = 9) or newly diagnosed type 2 diabetes (T2D, n = 6). Expression of 155 miRNAs was carried out using the TaqMan(R)MicroRNA Assays Human Panel Early Access Kit (Applied Biosystems, Darmstadt, Germany). We identified expression of 106 (68%) miRNAs in human omental and SC adipose tissue. There was no miRNA exclusively expressed in either fat depot, suggesting common developmental origin of both fat depots. Sixteen miRNAs (4 in NGT, 12 in T2D group) showed a significant fat depot specific expression pattern. We identified significant correlations between the expression of miRNA-17-5p, -132, -99a, -134, 181a, -145, -197 and both adipose tissue morphology and key metabolic parameters, including visceral fat area, HbA(1c), fasting plasma glucose, and circulating leptin, adiponectin, interleukin-6. In conclusion, microRNA expression differences may contribute to intrinsic differences between omental and subcutaneous adipose tissue. In addition, human adipose tissue miRNA expression correlates with adipocyte phenotype, parameters of obesity and glucose metabolism.Nora KlötingSusan BertholdPeter KovacsMichael R SchönMathias FasshauerKaren RuschkeMichael StumvollMatthias BlüherPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4699 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nora Klöting Susan Berthold Peter Kovacs Michael R Schön Mathias Fasshauer Karen Ruschke Michael Stumvoll Matthias Blüher MicroRNA expression in human omental and subcutaneous adipose tissue.
description	MicroRNAs (miRNAs) are small non-coding RNAs, that play important regulatory roles in a variety of biological processes, including development, differentiation, apoptosis, and metabolism. In mammals, miRNAs have been shown to modulate adipocyte differentiation. Therefore, we performed a global miRNA gene expression assay in different fat depots of overweight and obese individuals to investigate whether miRNA expression in human adipose tissue is fat-depot specific and associated with parameters of obesity and glucose metabolism. Paired samples of abdominal subcutaneous (SC) and intraabdominal omental adipose tissue were obtained from fifteen individuals with either normal glucose tolerance (NGT, n = 9) or newly diagnosed type 2 diabetes (T2D, n = 6). Expression of 155 miRNAs was carried out using the TaqMan(R)MicroRNA Assays Human Panel Early Access Kit (Applied Biosystems, Darmstadt, Germany). We identified expression of 106 (68%) miRNAs in human omental and SC adipose tissue. There was no miRNA exclusively expressed in either fat depot, suggesting common developmental origin of both fat depots. Sixteen miRNAs (4 in NGT, 12 in T2D group) showed a significant fat depot specific expression pattern. We identified significant correlations between the expression of miRNA-17-5p, -132, -99a, -134, 181a, -145, -197 and both adipose tissue morphology and key metabolic parameters, including visceral fat area, HbA(1c), fasting plasma glucose, and circulating leptin, adiponectin, interleukin-6. In conclusion, microRNA expression differences may contribute to intrinsic differences between omental and subcutaneous adipose tissue. In addition, human adipose tissue miRNA expression correlates with adipocyte phenotype, parameters of obesity and glucose metabolism.
format	article
author	Nora Klöting Susan Berthold Peter Kovacs Michael R Schön Mathias Fasshauer Karen Ruschke Michael Stumvoll Matthias Blüher
author_facet	Nora Klöting Susan Berthold Peter Kovacs Michael R Schön Mathias Fasshauer Karen Ruschke Michael Stumvoll Matthias Blüher
author_sort	Nora Klöting
title	MicroRNA expression in human omental and subcutaneous adipose tissue.
title_short	MicroRNA expression in human omental and subcutaneous adipose tissue.
title_full	MicroRNA expression in human omental and subcutaneous adipose tissue.
title_fullStr	MicroRNA expression in human omental and subcutaneous adipose tissue.
title_full_unstemmed	MicroRNA expression in human omental and subcutaneous adipose tissue.
title_sort	microrna expression in human omental and subcutaneous adipose tissue.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/c778e0a6c5a04b689b62d6a759af6789
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MicroRNA expression in human omental and subcutaneous adipose tissue.

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