The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain

Abstract Molecular epidemiology of circulating clinical isolates is crucial to improve prevention strategies. The Spanish Working Group on multidrug resistant tuberculosis (MDR-TB) is a network that monitors the MDR-TB isolates in Spain since 1998. The aim of this study was to present the study of t...

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Auteurs principaux:	María José Iglesias, Daniel Ibarz, Alberto Cebollada, Jéssica Comín, María Soledad Jiménez, María C. Vázquez, Sofía Samper, The Spanish Working Group on MDRTB
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2020
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/c77acb1b6a754a2f95a28bf081cf2be7
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spelling	oai:doaj.org-article:c77acb1b6a754a2f95a28bf081cf2be72021-12-02T11:41:41ZThe value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain10.1038/s41598-020-77249-x2045-2322https://doaj.org/article/c77acb1b6a754a2f95a28bf081cf2be72020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77249-xhttps://doaj.org/toc/2045-2322Abstract Molecular epidemiology of circulating clinical isolates is crucial to improve prevention strategies. The Spanish Working Group on multidrug resistant tuberculosis (MDR-TB) is a network that monitors the MDR-TB isolates in Spain since 1998. The aim of this study was to present the study of the MDR-TB and extensively drug-resistant tuberculosis (XDR-TB) patterns in Spain using the different recommended genotyping methods over time by a national coordinated system. Based on the proposed genotyping methods in the European Union until 2018, the preservation of one method, MIRU-VNTR, applied to selected clustered strains permitted to maintain our study open for 20 years. The distribution of demographic, clinical and epidemiological characteristics of clustered and non-clustered cases of MDR/XDR tuberculosis with proportion differences as assessed by Pearson’s chi-squared or Fisher’s exact test was compared. The differences in the quantitative variables using the Student's-t test and the Mann–Whitney U test were evaluated. The results obtained showed a total of 48.4% of the cases grouped in 77 clusters. Younger age groups, having a known TB case contact (10.2% vs 4.7%) and XDR-TB (16.5% vs 1.8%) were significantly associated with clustering. The largest cluster corresponded to a Mycobacterium bovis strain mainly spread during the nineties. A total of 68.4% of the clusters detected were distributed among the different Spanish regions and six clusters involving 104 cases were grouped in 17 and 18 years. Comparison of the genotypes obtained with those European genotypes included in The European Surveillance System (TESSy) showed that 87 cases had become part of 20 European clusters. The continuity of MDR strain genotyping in time has offered a widespread picture of the situation that allows better management of this public health problem. It also shows the advantage of maintaining one genotyping method over time, which allowed the comparison between ancient, present and future samples.María José IglesiasDaniel IbarzAlberto CebolladaJéssica ComínMaría Soledad JiménezMaría C. VázquezSofía SamperThe Spanish Working Group on MDRTBNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María José Iglesias Daniel Ibarz Alberto Cebollada Jéssica Comín María Soledad Jiménez María C. Vázquez Sofía Samper The Spanish Working Group on MDRTB The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain
description	Abstract Molecular epidemiology of circulating clinical isolates is crucial to improve prevention strategies. The Spanish Working Group on multidrug resistant tuberculosis (MDR-TB) is a network that monitors the MDR-TB isolates in Spain since 1998. The aim of this study was to present the study of the MDR-TB and extensively drug-resistant tuberculosis (XDR-TB) patterns in Spain using the different recommended genotyping methods over time by a national coordinated system. Based on the proposed genotyping methods in the European Union until 2018, the preservation of one method, MIRU-VNTR, applied to selected clustered strains permitted to maintain our study open for 20 years. The distribution of demographic, clinical and epidemiological characteristics of clustered and non-clustered cases of MDR/XDR tuberculosis with proportion differences as assessed by Pearson’s chi-squared or Fisher’s exact test was compared. The differences in the quantitative variables using the Student's-t test and the Mann–Whitney U test were evaluated. The results obtained showed a total of 48.4% of the cases grouped in 77 clusters. Younger age groups, having a known TB case contact (10.2% vs 4.7%) and XDR-TB (16.5% vs 1.8%) were significantly associated with clustering. The largest cluster corresponded to a Mycobacterium bovis strain mainly spread during the nineties. A total of 68.4% of the clusters detected were distributed among the different Spanish regions and six clusters involving 104 cases were grouped in 17 and 18 years. Comparison of the genotypes obtained with those European genotypes included in The European Surveillance System (TESSy) showed that 87 cases had become part of 20 European clusters. The continuity of MDR strain genotyping in time has offered a widespread picture of the situation that allows better management of this public health problem. It also shows the advantage of maintaining one genotyping method over time, which allowed the comparison between ancient, present and future samples.
format	article
author	María José Iglesias Daniel Ibarz Alberto Cebollada Jéssica Comín María Soledad Jiménez María C. Vázquez Sofía Samper The Spanish Working Group on MDRTB
author_facet	María José Iglesias Daniel Ibarz Alberto Cebollada Jéssica Comín María Soledad Jiménez María C. Vázquez Sofía Samper The Spanish Working Group on MDRTB
author_sort	María José Iglesias
title	The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain
title_short	The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain
title_full	The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain
title_fullStr	The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain
title_full_unstemmed	The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain
title_sort	value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in spain
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c77acb1b6a754a2f95a28bf081cf2be7
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The value of the continuous genotyping of multi-drug resistant tuberculosis over 20 years in Spain

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