Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity

Pannerselvam Balashanmugam, Pudupalayam Thangavelu Kalaichelvan Centre for Advanced Studies in Botany, University of Madras, Chennai, India Abstract: The present study reports the green synthesis of silver nanoparticles (AgNPs) from silver precursor using a plant biomaterial, Cassia roxburghii DC....

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Autores principales:	Balashanmugam P, Kalaichelvan PT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c7866fe92ad742febc36b4aee3597130
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spelling	oai:doaj.org-article:c7866fe92ad742febc36b4aee35971302021-12-02T01:32:50ZBiosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity1178-2013https://doaj.org/article/c7866fe92ad742febc36b4aee35971302015-10-01T00:00:00Zhttps://www.dovepress.com/biosynthesis-characterization-of-silver-nanoparticles-using-cassia-rox-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pannerselvam Balashanmugam, Pudupalayam Thangavelu Kalaichelvan Centre for Advanced Studies in Botany, University of Madras, Chennai, India Abstract: The present study reports the green synthesis of silver nanoparticles (AgNPs) from silver precursor using a plant biomaterial, Cassia roxburghii DC., aqueous extract. The AgNPs were synthesized from the shade-dried leaf extract and assessed for their stability; they elucidated characteristics under UV–visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy dispersive X-ray spectroscopy. The synthesized AgNPs exhibited a maximum absorption at 430 nm, and the X-ray diffraction patterns showed that they were crystal in nature. Fourier transform infrared spectroscopy analysis confirmed the conversion of Ag+ ions to AgNPs due to the reduction by capping material of plant extract. The HR-TEM analysis revealed that they are spherical ranging from 10 nm to 30 nm. The spot EDAX analysis showed the presence of silver atoms. In addition, AgNPs were evaluated for their antibacterial activity against six different pathogenic bacteria: three Gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus, and three Gram-negative bacteria, Pseudomonas aeruginosa, Escherichia coli, and Enterobacter aerogenes. They were highly sensitive to AgNPs, whereas less sensitive to AgNO3. Furthermore, the green synthesized AgNPs were immobilized on cotton fabrics and screened for antibacterial activity. The immobilized AgNPs on cotton cloth showed high antibacterial activity. Therefore, they could be a feasible alternative source in treating wounds or may help in replacing pharmaceutical band-aids. Keywords: bioreduction, stability, immobilization, cotton cloth, minimum inhibitory concentrationBalashanmugam PKalaichelvan PTDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Supplement 1 Challenges in biomaterials research, Pp 87-97 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Balashanmugam P Kalaichelvan PT Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity
description	Pannerselvam Balashanmugam, Pudupalayam Thangavelu Kalaichelvan Centre for Advanced Studies in Botany, University of Madras, Chennai, India Abstract: The present study reports the green synthesis of silver nanoparticles (AgNPs) from silver precursor using a plant biomaterial, Cassia roxburghii DC., aqueous extract. The AgNPs were synthesized from the shade-dried leaf extract and assessed for their stability; they elucidated characteristics under UV–visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy dispersive X-ray spectroscopy. The synthesized AgNPs exhibited a maximum absorption at 430 nm, and the X-ray diffraction patterns showed that they were crystal in nature. Fourier transform infrared spectroscopy analysis confirmed the conversion of Ag+ ions to AgNPs due to the reduction by capping material of plant extract. The HR-TEM analysis revealed that they are spherical ranging from 10 nm to 30 nm. The spot EDAX analysis showed the presence of silver atoms. In addition, AgNPs were evaluated for their antibacterial activity against six different pathogenic bacteria: three Gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus, and three Gram-negative bacteria, Pseudomonas aeruginosa, Escherichia coli, and Enterobacter aerogenes. They were highly sensitive to AgNPs, whereas less sensitive to AgNO3. Furthermore, the green synthesized AgNPs were immobilized on cotton fabrics and screened for antibacterial activity. The immobilized AgNPs on cotton cloth showed high antibacterial activity. Therefore, they could be a feasible alternative source in treating wounds or may help in replacing pharmaceutical band-aids. Keywords: bioreduction, stability, immobilization, cotton cloth, minimum inhibitory concentration
format	article
author	Balashanmugam P Kalaichelvan PT
author_facet	Balashanmugam P Kalaichelvan PT
author_sort	Balashanmugam P
title	Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity
title_short	Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity
title_full	Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity
title_fullStr	Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity
title_full_unstemmed	Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity
title_sort	biosynthesis characterization of silver nanoparticles using cassia roxburghii dc. aqueous extract, and coated on cotton cloth for effective antibacterial activity
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/c7866fe92ad742febc36b4aee3597130
work_keys_str_mv	AT balashanmugamp biosynthesischaracterizationofsilvernanoparticlesusingcassiaroxburghiidcaqueousextractandcoatedoncottonclothforeffectiveantibacterialactivity AT kalaichelvanpt biosynthesischaracterizationofsilvernanoparticlesusingcassiaroxburghiidcaqueousextractandcoatedoncottonclothforeffectiveantibacterialactivity
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Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity

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