STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM

Сurrent allergen-specific immunotherapy (AIT) is the most pathogenetically justified method of the IgE-dependent treatment of allergic diseases. Subcutaneous (SCIT) and sublingual (SLIT) AIT are currently used in wide clinical practice. Sublingual immunotherapy is characterized by the convenience of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: V. M. Berzhets, S. V. Khlgatian, S. Yu. Petrova, V. A. Khruleva, M. A. Efremov, A. B. Vasilyeva, N. S. Petrova, V. A. Akutina
Formato: article
Lenguaje:RU
Publicado: SPb RAACI 2018
Materias:
Acceso en línea:https://doaj.org/article/c78cae1c97fc4ba699fd15fb0aba2096
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:c78cae1c97fc4ba699fd15fb0aba2096
record_format dspace
spelling oai:doaj.org-article:c78cae1c97fc4ba699fd15fb0aba20962021-11-18T08:03:47ZSTUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM1563-06252313-741X10.15789/1563-0625-2018-4-597-600https://doaj.org/article/c78cae1c97fc4ba699fd15fb0aba20962018-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1568https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XСurrent allergen-specific immunotherapy (AIT) is the most pathogenetically justified method of the IgE-dependent treatment of allergic diseases. Subcutaneous (SCIT) and sublingual (SLIT) AIT are currently used in wide clinical practice. Sublingual immunotherapy is characterized by the convenience of use, decreased incidence of systemic and local side effects, and proven clinical efficacy. However, sublingual form of home dust mite allergen for AIT is not yet produced in Russia. Previously, the technology for sublingual form of D. farinae (Der f) mites allergen was developed in our laboratory, and this extract was shown to be non-toxic and free of any significant side effects. We obtained a sublingual form of mixed D. pteronyssinus (Der р)/D. farina mites allergen for sublingually allergen-specific immunotherapy. We studied physico-chemical and immunobiological properties of the Der р and Der f mites phenol-free water-salt mixed extract (WWSME). WWSME was studied after sterilizing filtration. WWSME was analyzed by electrophoresis in polyacrylamide gel in the presence of dodecyl-sodium sulfate and reverse-phase high-performance liquid chromatography. ELISA was performed with polyclonal rabbit serum, obtained by sensitization of animals with recombinant Der f1 and Der f2 allergens. ELISA shows positive reaction of WWSME with IgG-antibodies of rabbits. We studied acute and chronic toxicity of received mixed allergen in laboratory animals. Pathomorphological studies have shown that WWSME has no detectable toxicity and does not cause local irritation in the animals. The WWSME product contains native proteins, retains immunogenic properties, is non-toxic and does not cause significant side effects. This extract can be used as the basis for designing a sublingual form of dust mite mixed allergen, which is especially promising for pediatric applications.V. M. BerzhetsS. V. KhlgatianS. Yu. PetrovaV. A. KhrulevaM. A. EfremovA. B. VasilyevaN. S. PetrovaV. A. AkutinaSPb RAACIarticlehome dust mite allergensdermatophagoides pteronyssinusdermatophagoides farinaesublingual formallergyallergenspecific immunotherapyImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 4, Pp 597-600 (2018)
institution DOAJ
collection DOAJ
language RU
topic home dust mite allergens
dermatophagoides pteronyssinus
dermatophagoides farinae
sublingual form
allergy
allergenspecific immunotherapy
Immunologic diseases. Allergy
RC581-607
spellingShingle home dust mite allergens
dermatophagoides pteronyssinus
dermatophagoides farinae
sublingual form
allergy
allergenspecific immunotherapy
Immunologic diseases. Allergy
RC581-607
V. M. Berzhets
S. V. Khlgatian
S. Yu. Petrova
V. A. Khruleva
M. A. Efremov
A. B. Vasilyeva
N. S. Petrova
V. A. Akutina
STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM
description Сurrent allergen-specific immunotherapy (AIT) is the most pathogenetically justified method of the IgE-dependent treatment of allergic diseases. Subcutaneous (SCIT) and sublingual (SLIT) AIT are currently used in wide clinical practice. Sublingual immunotherapy is characterized by the convenience of use, decreased incidence of systemic and local side effects, and proven clinical efficacy. However, sublingual form of home dust mite allergen for AIT is not yet produced in Russia. Previously, the technology for sublingual form of D. farinae (Der f) mites allergen was developed in our laboratory, and this extract was shown to be non-toxic and free of any significant side effects. We obtained a sublingual form of mixed D. pteronyssinus (Der р)/D. farina mites allergen for sublingually allergen-specific immunotherapy. We studied physico-chemical and immunobiological properties of the Der р and Der f mites phenol-free water-salt mixed extract (WWSME). WWSME was studied after sterilizing filtration. WWSME was analyzed by electrophoresis in polyacrylamide gel in the presence of dodecyl-sodium sulfate and reverse-phase high-performance liquid chromatography. ELISA was performed with polyclonal rabbit serum, obtained by sensitization of animals with recombinant Der f1 and Der f2 allergens. ELISA shows positive reaction of WWSME with IgG-antibodies of rabbits. We studied acute and chronic toxicity of received mixed allergen in laboratory animals. Pathomorphological studies have shown that WWSME has no detectable toxicity and does not cause local irritation in the animals. The WWSME product contains native proteins, retains immunogenic properties, is non-toxic and does not cause significant side effects. This extract can be used as the basis for designing a sublingual form of dust mite mixed allergen, which is especially promising for pediatric applications.
format article
author V. M. Berzhets
S. V. Khlgatian
S. Yu. Petrova
V. A. Khruleva
M. A. Efremov
A. B. Vasilyeva
N. S. Petrova
V. A. Akutina
author_facet V. M. Berzhets
S. V. Khlgatian
S. Yu. Petrova
V. A. Khruleva
M. A. Efremov
A. B. Vasilyeva
N. S. Petrova
V. A. Akutina
author_sort V. M. Berzhets
title STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM
title_short STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM
title_full STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM
title_fullStr STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM
title_full_unstemmed STUDY OF THE PROPERTIES OF THE MIXED WATER/SALT HOUSE DUST MITE ALLERGEN AIMING TO DESIGN ITS SUBLINGUAL FORM
title_sort study of the properties of the mixed water/salt house dust mite allergen aiming to design its sublingual form
publisher SPb RAACI
publishDate 2018
url https://doaj.org/article/c78cae1c97fc4ba699fd15fb0aba2096
work_keys_str_mv AT vmberzhets studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT svkhlgatian studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT syupetrova studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT vakhruleva studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT maefremov studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT abvasilyeva studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT nspetrova studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
AT vaakutina studyofthepropertiesofthemixedwatersalthousedustmiteallergenaimingtodesignitssublingualform
_version_ 1718422381110755328