$Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants$
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Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants

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Background: Bioactive compounds were isolated from the fruit of S. elaeagnifolium, as it has a high potential for functional foods or pharmaceutical products development. Objectives: In this study bioguided fractionation a methanolic extract from S. elaeagnifolium fruit was carried out to evaluate...

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Autores principales:	Leobardo HERNÁNDEZ O., Pilar CARRANZA R., Luis Enrique COBOS P., Lluvia Itzel LÓPEZ L., Juan Alberto ASCASIO V., Sonia Yesenia SILVA B.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2017
Materias:	Solanum fractionation bioguided cell line tumor Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/c78d2f74b06649fd8ed56c5a446ed095
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spelling	oai:doaj.org-article:c78d2f74b06649fd8ed56c5a446ed0952021-11-19T04:09:04ZBioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants0121-40042145-2660https://doaj.org/article/c78d2f74b06649fd8ed56c5a446ed0952017-11-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/327108https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: Bioactive compounds were isolated from the fruit of S. elaeagnifolium, as it has a high potential for functional foods or pharmaceutical products development. Objectives: In this study bioguided fractionation a methanolic extract from S. elaeagnifolium fruit was carried out to evaluate cytotoxicity and antitumoral potential on breast tumor explants. Methods: A microdilution method with A. salina was used to isolate bioactive compounds; fractionation was performed by vacuum liquid chromatography, and the monitoring from fractions was done by thin layer chromatography. Moreover, toxicity from fractions isolated in Vero, HeLa, and MCF-7 cell lines was assessed by MTT assay, as well as in breast tumor explants by Alamar blue assay. To partially identify compounds was realized a qualitative phytochemical analysis as well as a spectroscopic characterization by Rp-HPLC-MS. Results: Were promising, as it was observed that FVLC7 showed an LC50= 44.8 ± 3.5 ppm on HeLa cell line, while Vero and MCF-7 cell lines showed an LC50=80.0 ± 8.5 and LC50 >1000 ppm respectively. Also, an antitumor effect was found in breast tumor explants obtained from a patient in remission. Qualitative phytochemical analysis showed that FVLC7 contains alkaloids, coumarins, and sesquiterpene lactones, whereas characterization by Rp-HPLC-MS detected quinic acid, chlorogenic acid, dicafeoilquinic acid as well as presence an alkaloid. Conclusion: Therefore, was confirmed that active compounds isolated from S. elaeagnifolium possess antineoplastic potential and that A. salina test facilitates its selection. Leobardo HERNÁNDEZ O.Pilar CARRANZA R.Luis Enrique COBOS P.Lluvia Itzel LÓPEZ L.Juan Alberto ASCASIO V.Sonia Yesenia SILVA B.Universidad de AntioquiaarticleSolanumfractionationbioguidedcell linetumorFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 24, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Solanum fractionation bioguided cell line tumor Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Solanum fractionation bioguided cell line tumor Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Leobardo HERNÁNDEZ O. Pilar CARRANZA R. Luis Enrique COBOS P. Lluvia Itzel LÓPEZ L. Juan Alberto ASCASIO V. Sonia Yesenia SILVA B. Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
description	Background: Bioactive compounds were isolated from the fruit of S. elaeagnifolium, as it has a high potential for functional foods or pharmaceutical products development. Objectives: In this study bioguided fractionation a methanolic extract from S. elaeagnifolium fruit was carried out to evaluate cytotoxicity and antitumoral potential on breast tumor explants. Methods: A microdilution method with A. salina was used to isolate bioactive compounds; fractionation was performed by vacuum liquid chromatography, and the monitoring from fractions was done by thin layer chromatography. Moreover, toxicity from fractions isolated in Vero, HeLa, and MCF-7 cell lines was assessed by MTT assay, as well as in breast tumor explants by Alamar blue assay. To partially identify compounds was realized a qualitative phytochemical analysis as well as a spectroscopic characterization by Rp-HPLC-MS. Results: Were promising, as it was observed that FVLC7 showed an LC50= 44.8 ± 3.5 ppm on HeLa cell line, while Vero and MCF-7 cell lines showed an LC50=80.0 ± 8.5 and LC50 >1000 ppm respectively. Also, an antitumor effect was found in breast tumor explants obtained from a patient in remission. Qualitative phytochemical analysis showed that FVLC7 contains alkaloids, coumarins, and sesquiterpene lactones, whereas characterization by Rp-HPLC-MS detected quinic acid, chlorogenic acid, dicafeoilquinic acid as well as presence an alkaloid. Conclusion: Therefore, was confirmed that active compounds isolated from S. elaeagnifolium possess antineoplastic potential and that A. salina test facilitates its selection.
format	article
author	Leobardo HERNÁNDEZ O. Pilar CARRANZA R. Luis Enrique COBOS P. Lluvia Itzel LÓPEZ L. Juan Alberto ASCASIO V. Sonia Yesenia SILVA B.
author_facet	Leobardo HERNÁNDEZ O. Pilar CARRANZA R. Luis Enrique COBOS P. Lluvia Itzel LÓPEZ L. Juan Alberto ASCASIO V. Sonia Yesenia SILVA B.
author_sort	Leobardo HERNÁNDEZ O.
title	Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
title_short	Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
title_full	Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
title_fullStr	Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
title_full_unstemmed	Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
title_sort	bioguided fractionation from solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
publisher	Universidad de Antioquia
publishDate	2017
url	https://doaj.org/article/c78d2f74b06649fd8ed56c5a446ed095
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Bioguided fractionation from Solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants

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