Antimicrobial Activity and Synergy Investigation of <i>Hypericum scabrum</i> Essential Oil with Antifungal Drugs
The chemical composition of Lebanese <i>Hypericum scabrum</i> essential oil (EO) was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GG-MS). Its antimicrobial activity was evaluated by determining its minimal inhibitory concentrations (MICs) against a Gram-n...
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| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/c79262c6e8df49a3827ffd7e176889a6 |
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| Summary: | The chemical composition of Lebanese <i>Hypericum scabrum</i> essential oil (EO) was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GG-MS). Its antimicrobial activity was evaluated by determining its minimal inhibitory concentrations (MICs) against a Gram-negative and a Gram-positive bacterium, one yeast, and five dermatophytes. <i>H. scabrum</i> EO was most active on filamentous fungi (MIC values of 32–64 µg/mL). Synergy within the oil was investigated by testing each of the following major components on <i>Trichophyton rubrum</i>: <i>α</i>-pinene, limonene, myrcene, <i>β</i>-pinene and nonane, as well as a reconstructed EO. The antifungal activity of the natural oil could not be reached, meaning that its activity might be due, in part, to minor constituent(s). The interactions between <i>H. scabrum</i> EO and commercially available antifungals were assessed by the checkerboard test. A synergistic effect was revealed in the combination of the EO with amphotericin B. |
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