Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo

Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for i...

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Autores principales:	Jes Dreier, Marco Castello, Giovanna Coceano, Rodrigo Cáceres, Julie Plastino, Giuseppe Vicidomini, Ilaria Testa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c798aa953bca43349819cb9b88e61010
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spelling	oai:doaj.org-article:c798aa953bca43349819cb9b88e610102021-12-02T17:01:45ZSmart scanning for low-illumination and fast RESOLFT nanoscopy in vivo10.1038/s41467-019-08442-42041-1723https://doaj.org/article/c798aa953bca43349819cb9b88e610102019-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08442-4https://doaj.org/toc/2041-1723Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for in vivo imaging.Jes DreierMarco CastelloGiovanna CoceanoRodrigo CáceresJulie PlastinoGiuseppe VicidominiIlaria TestaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jes Dreier Marco Castello Giovanna Coceano Rodrigo Cáceres Julie Plastino Giuseppe Vicidomini Ilaria Testa Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
description	Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for in vivo imaging.
format	article
author	Jes Dreier Marco Castello Giovanna Coceano Rodrigo Cáceres Julie Plastino Giuseppe Vicidomini Ilaria Testa
author_facet	Jes Dreier Marco Castello Giovanna Coceano Rodrigo Cáceres Julie Plastino Giuseppe Vicidomini Ilaria Testa
author_sort	Jes Dreier
title	Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
title_short	Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
title_full	Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
title_fullStr	Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
title_full_unstemmed	Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
title_sort	smart scanning for low-illumination and fast resolft nanoscopy in vivo
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c798aa953bca43349819cb9b88e61010
work_keys_str_mv	AT jesdreier smartscanningforlowilluminationandfastresolftnanoscopyinvivo AT marcocastello smartscanningforlowilluminationandfastresolftnanoscopyinvivo AT giovannacoceano smartscanningforlowilluminationandfastresolftnanoscopyinvivo AT rodrigocaceres smartscanningforlowilluminationandfastresolftnanoscopyinvivo AT julieplastino smartscanningforlowilluminationandfastresolftnanoscopyinvivo AT giuseppevicidomini smartscanningforlowilluminationandfastresolftnanoscopyinvivo AT ilariatesta smartscanningforlowilluminationandfastresolftnanoscopyinvivo
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Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo

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