Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.

The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies. Elicitors like cadmium chloride, aluminium chloride and chitosan, prec...

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Autores principales:	Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, Markandan Manickavasagam
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c79bfb6f332742f7a79ab0fc72350bb5
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spelling	oai:doaj.org-article:c79bfb6f332742f7a79ab0fc72350bb52021-11-25T06:06:07ZEnhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.1932-620310.1371/journal.pone.0104005https://doaj.org/article/c79bfb6f332742f7a79ab0fc72350bb52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25089711/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies. Elicitors like cadmium chloride, aluminium chloride and chitosan, precursors such as cholesterol, mevalonic acid and squalene were examined. Maximum total withanolides detected [withanolide A (7606.75 mg), withanolide B (4826.05 mg), withaferin A (3732.81 mg), withanone (6538.65 mg), 12 deoxy withanstramonolide (3176.63 mg), withanoside IV (2623.21 mg) and withanoside V (2861.18 mg)] were achieved in the combined treatment of chitosan (100 mg/l) and squalene (6 mM) along with 1 mg/l picloram, 0.5 mg/l KN, 200 mg/l L-glutamine and 5% sucrose in culture at 4 h and 48 h exposure times respectively on 28th day of culture in bioreactor. We obtained higher concentrations of total withanolides in shake-flask culture (2.13-fold) as well as bioreactor (1.66-fold) when compared to control treatments. This optimized protocol can be utilized for commercial level production of withanolides from suspension culture using industrial bioreactors in a short culture period.Ganeshan SivanandhanNatesan SelvarajAndy GanapathiMarkandan ManickavasagamPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104005 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ganeshan Sivanandhan Natesan Selvaraj Andy Ganapathi Markandan Manickavasagam Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
description	The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies. Elicitors like cadmium chloride, aluminium chloride and chitosan, precursors such as cholesterol, mevalonic acid and squalene were examined. Maximum total withanolides detected [withanolide A (7606.75 mg), withanolide B (4826.05 mg), withaferin A (3732.81 mg), withanone (6538.65 mg), 12 deoxy withanstramonolide (3176.63 mg), withanoside IV (2623.21 mg) and withanoside V (2861.18 mg)] were achieved in the combined treatment of chitosan (100 mg/l) and squalene (6 mM) along with 1 mg/l picloram, 0.5 mg/l KN, 200 mg/l L-glutamine and 5% sucrose in culture at 4 h and 48 h exposure times respectively on 28th day of culture in bioreactor. We obtained higher concentrations of total withanolides in shake-flask culture (2.13-fold) as well as bioreactor (1.66-fold) when compared to control treatments. This optimized protocol can be utilized for commercial level production of withanolides from suspension culture using industrial bioreactors in a short culture period.
format	article
author	Ganeshan Sivanandhan Natesan Selvaraj Andy Ganapathi Markandan Manickavasagam
author_facet	Ganeshan Sivanandhan Natesan Selvaraj Andy Ganapathi Markandan Manickavasagam
author_sort	Ganeshan Sivanandhan
title	Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
title_short	Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
title_full	Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
title_fullStr	Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
title_full_unstemmed	Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.
title_sort	enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of withania somnifera (l.) dunal in shake-flask culture and bioreactor.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c79bfb6f332742f7a79ab0fc72350bb5
work_keys_str_mv	AT ganeshansivanandhan enhancedbiosynthesisofwithanolidesbyelicitationandprecursorfeedingincellsuspensioncultureofwithaniasomniferaldunalinshakeflaskcultureandbioreactor AT natesanselvaraj enhancedbiosynthesisofwithanolidesbyelicitationandprecursorfeedingincellsuspensioncultureofwithaniasomniferaldunalinshakeflaskcultureandbioreactor AT andyganapathi enhancedbiosynthesisofwithanolidesbyelicitationandprecursorfeedingincellsuspensioncultureofwithaniasomniferaldunalinshakeflaskcultureandbioreactor AT markandanmanickavasagam enhancedbiosynthesisofwithanolidesbyelicitationandprecursorfeedingincellsuspensioncultureofwithaniasomniferaldunalinshakeflaskcultureandbioreactor
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Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor.

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