New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki

Abstract Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrob...

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Autores principales: Taisiya A. Voronina, Alexander A. Nesmelov, Sabina A. Kondratyeva, Ruslan M. Deviatiiarov, Yugo Miyata, Shoko Tokumoto, Richard Cornette, Oleg A. Gusev, Takahiro Kikawada, Elena I. Shagimardanova
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Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/c79d9291961944d7ad61a17de592df2e
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spelling oai:doaj.org-article:c79d9291961944d7ad61a17de592df2e2021-12-02T15:32:59ZNew group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki10.1038/s41598-020-68330-62045-2322https://doaj.org/article/c79d9291961944d7ad61a17de592df2e2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68330-6https://doaj.org/toc/2045-2322Abstract Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrobiosis. While several such clusters represent orthologues of known genes, there is a distinct set of genes unique for P. vanderplanki. These include Lea-Island-Located (LIL) genes with no known orthologues except two of LEA genes of P. vanderplanki, PvLea1 and PvLea3. However, PvLIL proteins lack typical features of LEA such as the state of intrinsic disorder, hydrophilicity and characteristic LEA_4 motif. They possess four to five transmembrane domains each and we confirmed membrane targeting for three PvLILs. Conserved amino acids in PvLIL are located in transmembrane domains or nearby. PvLEA1 and PvLEA3 proteins are chimeras combining LEA-like parts and transmembrane domains, shared with PvLIL proteins. We have found that PvLil genes are highly upregulated during anhydrobiosis induction both in larvae of P. vanderplanki and P. vanderplanki-derived cultured cell line, Pv11. Thus, PvLil are a new intriguing group of genes that are likely to be associated with anhydrobiosis due to their common origin with some LEA genes and their induction during anhydrobiosis.Taisiya A. VoroninaAlexander A. NesmelovSabina A. KondratyevaRuslan M. DeviatiiarovYugo MiyataShoko TokumotoRichard CornetteOleg A. GusevTakahiro KikawadaElena I. ShagimardanovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Taisiya A. Voronina
Alexander A. Nesmelov
Sabina A. Kondratyeva
Ruslan M. Deviatiiarov
Yugo Miyata
Shoko Tokumoto
Richard Cornette
Oleg A. Gusev
Takahiro Kikawada
Elena I. Shagimardanova
New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
description Abstract Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrobiosis. While several such clusters represent orthologues of known genes, there is a distinct set of genes unique for P. vanderplanki. These include Lea-Island-Located (LIL) genes with no known orthologues except two of LEA genes of P. vanderplanki, PvLea1 and PvLea3. However, PvLIL proteins lack typical features of LEA such as the state of intrinsic disorder, hydrophilicity and characteristic LEA_4 motif. They possess four to five transmembrane domains each and we confirmed membrane targeting for three PvLILs. Conserved amino acids in PvLIL are located in transmembrane domains or nearby. PvLEA1 and PvLEA3 proteins are chimeras combining LEA-like parts and transmembrane domains, shared with PvLIL proteins. We have found that PvLil genes are highly upregulated during anhydrobiosis induction both in larvae of P. vanderplanki and P. vanderplanki-derived cultured cell line, Pv11. Thus, PvLil are a new intriguing group of genes that are likely to be associated with anhydrobiosis due to their common origin with some LEA genes and their induction during anhydrobiosis.
format article
author Taisiya A. Voronina
Alexander A. Nesmelov
Sabina A. Kondratyeva
Ruslan M. Deviatiiarov
Yugo Miyata
Shoko Tokumoto
Richard Cornette
Oleg A. Gusev
Takahiro Kikawada
Elena I. Shagimardanova
author_facet Taisiya A. Voronina
Alexander A. Nesmelov
Sabina A. Kondratyeva
Ruslan M. Deviatiiarov
Yugo Miyata
Shoko Tokumoto
Richard Cornette
Oleg A. Gusev
Takahiro Kikawada
Elena I. Shagimardanova
author_sort Taisiya A. Voronina
title New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_short New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_full New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_fullStr New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_full_unstemmed New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_sort new group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge polypedilum vanderplanki
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/c79d9291961944d7ad61a17de592df2e
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