High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.

<h4>Background</h4>Diarrheal disease is a leading cause of morbidity and mortality globally, especially in low- and middle-income countries. High-throughput and low-cost approaches to identify etiologic agents are needed to guide public health mitigation. Nanoliter-qPCR (nl-qPCR) is an a...

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Autores principales:	Katelyn E Flaherty, Jessica A Grembi, Vasavi V Ramachandran, Farhana Haque, Selina Khatun, Mahmudu Rahman, Stace Maples, Torben K Becker, Alfred M Spormann, Gary K Schoolnik, Andrew J Hryckowian, Eric J Nelson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c79f8c795ef84b57b14a7a0bd605625a
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spelling	oai:doaj.org-article:c79f8c795ef84b57b14a7a0bd605625a2021-12-02T20:17:26ZHigh-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.1932-620310.1371/journal.pone.0257708https://doaj.org/article/c79f8c795ef84b57b14a7a0bd605625a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257708https://doaj.org/toc/1932-6203<h4>Background</h4>Diarrheal disease is a leading cause of morbidity and mortality globally, especially in low- and middle-income countries. High-throughput and low-cost approaches to identify etiologic agents are needed to guide public health mitigation. Nanoliter-qPCR (nl-qPCR) is an attractive alternative to more expensive methods yet is nascent in application and without a proof-of-concept among hospitalized patients.<h4>Methods</h4>A census-based study was conducted among diarrheal patients admitted at two government hospitals in rural Bangladesh during a diarrheal outbreak period. DNA was extracted from stool samples and assayed by nl-qPCR for common bacterial, protozoan, and helminth enteropathogens as the primary outcome.<h4>Results</h4>A total of 961 patients were enrolled; stool samples were collected from 827 patients. Enteropathogens were detected in 69% of patient samples; More than one enteropathogen was detected in 32%. Enteropathogens most commonly detected were enteroaggregative Escherichia coli (26.0%), Shiga toxin-producing E.coli (18.3%), enterotoxigenic E. coli (15.5% heat stable toxin positive, 2.2% heat labile toxin positive), Shigella spp. (14.8%), and Vibrio cholerae (9.0%). Geospatial analysis revealed that the median number of pathogens per patient and the proportion of cases presenting with severe dehydration were greatest amongst patients residing closest to the study hospitals."<h4>Conclusions</h4>This study demonstrates a proof-of-concept for nl-qPCR as a high-throughput low-cost method for enteropathogen detection among hospitalized patients.Katelyn E FlahertyJessica A GrembiVasavi V RamachandranFarhana HaqueSelina KhatunMahmudu RahmanStace MaplesTorben K BeckerAlfred M SpormannGary K SchoolnikAndrew J HryckowianEric J NelsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257708 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katelyn E Flaherty Jessica A Grembi Vasavi V Ramachandran Farhana Haque Selina Khatun Mahmudu Rahman Stace Maples Torben K Becker Alfred M Spormann Gary K Schoolnik Andrew J Hryckowian Eric J Nelson High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.
description	<h4>Background</h4>Diarrheal disease is a leading cause of morbidity and mortality globally, especially in low- and middle-income countries. High-throughput and low-cost approaches to identify etiologic agents are needed to guide public health mitigation. Nanoliter-qPCR (nl-qPCR) is an attractive alternative to more expensive methods yet is nascent in application and without a proof-of-concept among hospitalized patients.<h4>Methods</h4>A census-based study was conducted among diarrheal patients admitted at two government hospitals in rural Bangladesh during a diarrheal outbreak period. DNA was extracted from stool samples and assayed by nl-qPCR for common bacterial, protozoan, and helminth enteropathogens as the primary outcome.<h4>Results</h4>A total of 961 patients were enrolled; stool samples were collected from 827 patients. Enteropathogens were detected in 69% of patient samples; More than one enteropathogen was detected in 32%. Enteropathogens most commonly detected were enteroaggregative Escherichia coli (26.0%), Shiga toxin-producing E.coli (18.3%), enterotoxigenic E. coli (15.5% heat stable toxin positive, 2.2% heat labile toxin positive), Shigella spp. (14.8%), and Vibrio cholerae (9.0%). Geospatial analysis revealed that the median number of pathogens per patient and the proportion of cases presenting with severe dehydration were greatest amongst patients residing closest to the study hospitals."<h4>Conclusions</h4>This study demonstrates a proof-of-concept for nl-qPCR as a high-throughput low-cost method for enteropathogen detection among hospitalized patients.
format	article
author	Katelyn E Flaherty Jessica A Grembi Vasavi V Ramachandran Farhana Haque Selina Khatun Mahmudu Rahman Stace Maples Torben K Becker Alfred M Spormann Gary K Schoolnik Andrew J Hryckowian Eric J Nelson
author_facet	Katelyn E Flaherty Jessica A Grembi Vasavi V Ramachandran Farhana Haque Selina Khatun Mahmudu Rahman Stace Maples Torben K Becker Alfred M Spormann Gary K Schoolnik Andrew J Hryckowian Eric J Nelson
author_sort	Katelyn E Flaherty
title	High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.
title_short	High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.
title_full	High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.
title_fullStr	High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.
title_full_unstemmed	High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.
title_sort	high-throughput low-cost nl-qpcr for enteropathogen detection: a proof-of-concept among hospitalized patients in bangladesh.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c79f8c795ef84b57b14a7a0bd605625a
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High-throughput low-cost nl-qPCR for enteropathogen detection: A proof-of-concept among hospitalized patients in Bangladesh.

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