Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.

<h4>Background/objective</h4>The Indianmeal moth Plodia interpunctella is a highly prevalent food pest in human dwellings, and has been shown to contain a number of allergens. So far, only one of these, the arginine kinase (Plo i 1) has been identified.<h4>Objective</h4>The a...

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Autores principales:	Elisabeth Hoflehner, Marina Binder, Wolfgang Hemmer, Vera Mahler, Raphael C Panzani, Reinhart Jarisch, Ursula Wiedermann, Michael Duchêne
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c79f9971a623402888c55e090e1c4864
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spelling	oai:doaj.org-article:c79f9971a623402888c55e090e1c48642021-11-18T07:10:54ZThioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.1932-620310.1371/journal.pone.0042026https://doaj.org/article/c79f9971a623402888c55e090e1c48642012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22844539/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background/objective</h4>The Indianmeal moth Plodia interpunctella is a highly prevalent food pest in human dwellings, and has been shown to contain a number of allergens. So far, only one of these, the arginine kinase (Plo i 1) has been identified.<h4>Objective</h4>The aim of this study was to identify further allergens and characterise these in comparison to Plo i 1.<h4>Method</h4>A cDNA library from whole adult P. interpunctella was screened with the serum of a patient with indoor allergy and IgE to moths, and thioredoxin was identified as an IgE-binding protein. Recombinant thioredoxin was generated in E. coli, and tested together with Plo i 1 and whole moth extracts in IgE immunoblots against a large panel of indoor allergic patients' sera. BALB/c mice were immunised with recombinant thioredoxin and Plo i 1, and antibody production, mediator release from RBL cells, T-cell proliferation and cytokine production were measured.<h4>Result</h4>For the first time a thioredoxin from an animal species was identified as allergen. About 8% of the sera from patients with IgE against moth extracts reacted with recombinant P. interpunctella thioredoxin, compared to 25% reacting with recombinant Plo i 1. In immunised BALB/c mice, the recombinant allergens both induced classical Th2-biased immune responses such as induction IgE and IgG1 antibodies, upregulation of IL-5 and IL-4 and basophil degranulation.<h4>Conclusion</h4>Thioredoxin from moths like Plo i 1 acts like a classical Type I allergen as do the thioredoxins from wheat or corn. This clearly supports the pan-allergen nature of thioredoxin. The designation Plo i 2 is suggested for the new P. interpunctella allergen.Elisabeth HoflehnerMarina BinderWolfgang HemmerVera MahlerRaphael C PanzaniReinhart JarischUrsula WiedermannMichael DuchênePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e42026 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisabeth Hoflehner Marina Binder Wolfgang Hemmer Vera Mahler Raphael C Panzani Reinhart Jarisch Ursula Wiedermann Michael Duchêne Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
description	<h4>Background/objective</h4>The Indianmeal moth Plodia interpunctella is a highly prevalent food pest in human dwellings, and has been shown to contain a number of allergens. So far, only one of these, the arginine kinase (Plo i 1) has been identified.<h4>Objective</h4>The aim of this study was to identify further allergens and characterise these in comparison to Plo i 1.<h4>Method</h4>A cDNA library from whole adult P. interpunctella was screened with the serum of a patient with indoor allergy and IgE to moths, and thioredoxin was identified as an IgE-binding protein. Recombinant thioredoxin was generated in E. coli, and tested together with Plo i 1 and whole moth extracts in IgE immunoblots against a large panel of indoor allergic patients' sera. BALB/c mice were immunised with recombinant thioredoxin and Plo i 1, and antibody production, mediator release from RBL cells, T-cell proliferation and cytokine production were measured.<h4>Result</h4>For the first time a thioredoxin from an animal species was identified as allergen. About 8% of the sera from patients with IgE against moth extracts reacted with recombinant P. interpunctella thioredoxin, compared to 25% reacting with recombinant Plo i 1. In immunised BALB/c mice, the recombinant allergens both induced classical Th2-biased immune responses such as induction IgE and IgG1 antibodies, upregulation of IL-5 and IL-4 and basophil degranulation.<h4>Conclusion</h4>Thioredoxin from moths like Plo i 1 acts like a classical Type I allergen as do the thioredoxins from wheat or corn. This clearly supports the pan-allergen nature of thioredoxin. The designation Plo i 2 is suggested for the new P. interpunctella allergen.
format	article
author	Elisabeth Hoflehner Marina Binder Wolfgang Hemmer Vera Mahler Raphael C Panzani Reinhart Jarisch Ursula Wiedermann Michael Duchêne
author_facet	Elisabeth Hoflehner Marina Binder Wolfgang Hemmer Vera Mahler Raphael C Panzani Reinhart Jarisch Ursula Wiedermann Michael Duchêne
author_sort	Elisabeth Hoflehner
title	Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
title_short	Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
title_full	Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
title_fullStr	Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
title_full_unstemmed	Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
title_sort	thioredoxin from the indianmeal moth plodia interpunctella: cloning and test of the allergenic potential in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c79f9971a623402888c55e090e1c4864
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Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.

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