Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway

Ruiting Shen,1,* Peng Yin,2,* Hua Yao,1,* Lu Chen,1 Xinyu Chang,1 Huanrong Li,1 Xiaolin Hou1 1Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China; 2Institute of Microbiology Chinese Academy of Sciences, Beijing, People&rsquo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shen R, Yin P, Yao H, Chen L, Chang X, Li H, Hou X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	punicalin nlrp3 pyroptosis ros Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c79fedc6eb7f41b499a597a2caf42632
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c79fedc6eb7f41b499a597a2caf42632
record_format	dspace
spelling	oai:doaj.org-article:c79fedc6eb7f41b499a597a2caf426322021-12-02T10:43:33ZPunicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway1178-7031https://doaj.org/article/c79fedc6eb7f41b499a597a2caf426322021-03-01T00:00:00Zhttps://www.dovepress.com/punicalin-ameliorates-cell-pyroptosis-induced-by-lpsatp-through-suppre-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Ruiting Shen,1,* Peng Yin,2,* Hua Yao,1,* Lu Chen,1 Xinyu Chang,1 Huanrong Li,1 Xiaolin Hou1 1Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China; 2Institute of Microbiology Chinese Academy of Sciences, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaolin Hou Tel +86 10-80799397Email hxlsx@163.comHuanrong Li Email lihuanrong1@126.comPurpose: Inflammation is the driving force of many inflammatory and autoimmune diseases, Pyroptosis is a process of cell death in response to excessive inflammation. Punicalin has been reported to have anti-inflammatory effects. However, the anti-pyroptosis is unknown. Hence, this study was aimed to research the inhibition of MG on LPS/ATP-induced pyroptosis in vitro.Methods: Lipopolysaccharide (LPS)/ATP were used to simulate mouse J774A.1 cells to mimic the inflammatory response and the role of punicalin was examined. The secretion of proinflammatory cytokines was analyzed using enzyme-linked immunosorbent assay (ELISA). The expression of nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC), caspase-1, and GSDMD-N in LPS/ATP-stimulated cells were examined by Western blot. N-acetylcysteine (NAC) was used to validate the role of Punicalin.Results: Punicalin significantly blocked the production of endogenous ROS, reduced LPS/ATP-induced activation of NLRP3, caspase 1, ASC and GSDMD-N, IL-1b and IL-18 protein levels. Furthermore, N-acetylcysteine (NAC), an ROS scavenger, inhibited the LPS/ATP-stimulated activation of NLRP3 inflammasome mediated inflammation and pyroptosis.Conclusion: Punicalin ameliorates LPS/ATP-induced pyroptosis in J774A.1 macrophages, the mechanism may involve downregulation of the ROS/NLRP3 inflammasome signaling pathway.Keywords: punicalin, NLRP3, pyroptosis, ROSShen RYin PYao HChen LChang XLi HHou XDove Medical Pressarticlepunicalinnlrp3pyroptosisrosPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 711-718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	punicalin nlrp3 pyroptosis ros Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	punicalin nlrp3 pyroptosis ros Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Shen R Yin P Yao H Chen L Chang X Li H Hou X Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway
description	Ruiting Shen,1,* Peng Yin,2,* Hua Yao,1,* Lu Chen,1 Xinyu Chang,1 Huanrong Li,1 Xiaolin Hou1 1Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China; 2Institute of Microbiology Chinese Academy of Sciences, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaolin Hou Tel +86 10-80799397Email hxlsx@163.comHuanrong Li Email lihuanrong1@126.comPurpose: Inflammation is the driving force of many inflammatory and autoimmune diseases, Pyroptosis is a process of cell death in response to excessive inflammation. Punicalin has been reported to have anti-inflammatory effects. However, the anti-pyroptosis is unknown. Hence, this study was aimed to research the inhibition of MG on LPS/ATP-induced pyroptosis in vitro.Methods: Lipopolysaccharide (LPS)/ATP were used to simulate mouse J774A.1 cells to mimic the inflammatory response and the role of punicalin was examined. The secretion of proinflammatory cytokines was analyzed using enzyme-linked immunosorbent assay (ELISA). The expression of nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC), caspase-1, and GSDMD-N in LPS/ATP-stimulated cells were examined by Western blot. N-acetylcysteine (NAC) was used to validate the role of Punicalin.Results: Punicalin significantly blocked the production of endogenous ROS, reduced LPS/ATP-induced activation of NLRP3, caspase 1, ASC and GSDMD-N, IL-1b and IL-18 protein levels. Furthermore, N-acetylcysteine (NAC), an ROS scavenger, inhibited the LPS/ATP-stimulated activation of NLRP3 inflammasome mediated inflammation and pyroptosis.Conclusion: Punicalin ameliorates LPS/ATP-induced pyroptosis in J774A.1 macrophages, the mechanism may involve downregulation of the ROS/NLRP3 inflammasome signaling pathway.Keywords: punicalin, NLRP3, pyroptosis, ROS
format	article
author	Shen R Yin P Yao H Chen L Chang X Li H Hou X
author_facet	Shen R Yin P Yao H Chen L Chang X Li H Hou X
author_sort	Shen R
title	Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway
title_short	Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway
title_full	Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway
title_fullStr	Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway
title_full_unstemmed	Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway
title_sort	punicalin ameliorates cell pyroptosis induced by lps/atp through suppression of ros/nlrp3 pathway
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/c79fedc6eb7f41b499a597a2caf42632
work_keys_str_mv	AT shenr punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway AT yinp punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway AT yaoh punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway AT chenl punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway AT changx punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway AT lih punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway AT houx punicalinamelioratescellpyroptosisinducedbylpsatpthroughsuppressionofrosnlrp3pathway
_version_	1718396853686370304

Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway

Ejemplares similares