Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect
The extracellular calcium-sensing receptor (CaSR) controls vital bone cell functions such as cell growth, differentiation and apoptosis. The binding of the native agonist (Ca<sup>2+</sup>) to CaSR activates the receptor, which undergoes structural changes that trigger a cascade of events...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c7a26232e69549ffba8a8a9ce092b027 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c7a26232e69549ffba8a8a9ce092b027 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c7a26232e69549ffba8a8a9ce092b0272021-11-25T16:52:09ZCa<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect10.3390/biom111115762218-273Xhttps://doaj.org/article/c7a26232e69549ffba8a8a9ce092b0272021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1576https://doaj.org/toc/2218-273XThe extracellular calcium-sensing receptor (CaSR) controls vital bone cell functions such as cell growth, differentiation and apoptosis. The binding of the native agonist (Ca<sup>2+</sup>) to CaSR activates the receptor, which undergoes structural changes that trigger a cascade of events along the cellular signaling pathways. Strontium (in the form of soluble salts) has been found to also be a CaSR agonist. The activation of the receptor by Sr<sup>2+</sup> is considered to be the major mechanism through which strontium exerts its anti-osteoporosis effect, mostly in postmenopausal women. Strontium-activated CaSR initiates a series of signal transduction events resulting in both osteoclast apoptosis and osteoblast differentiation, thus strengthening the bone tissue. The intimate mechanism of Sr<sup>2+</sup> activation of CaSR is still enigmatic. Herewith, by employing a combination of density functional theory (DFT) calculations and polarizable continuum model (PCM) computations, we have found that the Ca<sup>2+</sup> binding sites 1, 3, and 4 in the activated CaSR, although possessing a different number and type of protein ligands, overall structure and charge state, are all selective for Ca<sup>2+</sup> over Sr<sup>2+</sup>. The three binding sites, regardless of their structural differences, exhibit almost equal metal selectivity if they are flexible and have no geometrical constraints on the incoming Sr<sup>2+</sup>. In contrast to Ca<sup>2+</sup> and Sr<sup>2+</sup>, Mg<sup>2+</sup> constructs, when allowed to fully relax during the optimization process, adopt their stringent six-coordinated octahedral structure at the expense of detaching a one-backbone carbonyl ligand and shifting it to the second coordination layer of the metal. The binding of Mg<sup>2+</sup> and Sr<sup>2+</sup> to a rigid/inflexible calcium-designed binding pocket requires an additional energy penalty for the binding ion; however, the price for doing so (to be paid by Sr<sup>2+</sup>) is much less than that of Mg<sup>2+</sup>. The results obtained delineate the key factors controlling the competition between metal cations for the receptor and shed light on some aspects of strontium’s therapeutic effects.Diana CheshmedzhievaSonia IlievaEugene A. PermyakovSergei E. PermyakovTodor DudevMDPI AGarticlecalcium-sensing receptor (CaSR)Ca<sup>2+</sup>/Sr<sup>2+</sup> selectivityMg<sup>2+</sup>DFT/PCM calculationsCaSR agonistsosteoporosisMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1576, p 1576 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
calcium-sensing receptor (CaSR) Ca<sup>2+</sup>/Sr<sup>2+</sup> selectivity Mg<sup>2+</sup> DFT/PCM calculations CaSR agonists osteoporosis Microbiology QR1-502 |
| spellingShingle |
calcium-sensing receptor (CaSR) Ca<sup>2+</sup>/Sr<sup>2+</sup> selectivity Mg<sup>2+</sup> DFT/PCM calculations CaSR agonists osteoporosis Microbiology QR1-502 Diana Cheshmedzhieva Sonia Ilieva Eugene A. Permyakov Sergei E. Permyakov Todor Dudev Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect |
| description |
The extracellular calcium-sensing receptor (CaSR) controls vital bone cell functions such as cell growth, differentiation and apoptosis. The binding of the native agonist (Ca<sup>2+</sup>) to CaSR activates the receptor, which undergoes structural changes that trigger a cascade of events along the cellular signaling pathways. Strontium (in the form of soluble salts) has been found to also be a CaSR agonist. The activation of the receptor by Sr<sup>2+</sup> is considered to be the major mechanism through which strontium exerts its anti-osteoporosis effect, mostly in postmenopausal women. Strontium-activated CaSR initiates a series of signal transduction events resulting in both osteoclast apoptosis and osteoblast differentiation, thus strengthening the bone tissue. The intimate mechanism of Sr<sup>2+</sup> activation of CaSR is still enigmatic. Herewith, by employing a combination of density functional theory (DFT) calculations and polarizable continuum model (PCM) computations, we have found that the Ca<sup>2+</sup> binding sites 1, 3, and 4 in the activated CaSR, although possessing a different number and type of protein ligands, overall structure and charge state, are all selective for Ca<sup>2+</sup> over Sr<sup>2+</sup>. The three binding sites, regardless of their structural differences, exhibit almost equal metal selectivity if they are flexible and have no geometrical constraints on the incoming Sr<sup>2+</sup>. In contrast to Ca<sup>2+</sup> and Sr<sup>2+</sup>, Mg<sup>2+</sup> constructs, when allowed to fully relax during the optimization process, adopt their stringent six-coordinated octahedral structure at the expense of detaching a one-backbone carbonyl ligand and shifting it to the second coordination layer of the metal. The binding of Mg<sup>2+</sup> and Sr<sup>2+</sup> to a rigid/inflexible calcium-designed binding pocket requires an additional energy penalty for the binding ion; however, the price for doing so (to be paid by Sr<sup>2+</sup>) is much less than that of Mg<sup>2+</sup>. The results obtained delineate the key factors controlling the competition between metal cations for the receptor and shed light on some aspects of strontium’s therapeutic effects. |
| format |
article |
| author |
Diana Cheshmedzhieva Sonia Ilieva Eugene A. Permyakov Sergei E. Permyakov Todor Dudev |
| author_facet |
Diana Cheshmedzhieva Sonia Ilieva Eugene A. Permyakov Sergei E. Permyakov Todor Dudev |
| author_sort |
Diana Cheshmedzhieva |
| title |
Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect |
| title_short |
Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect |
| title_full |
Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect |
| title_fullStr |
Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect |
| title_full_unstemmed |
Ca<sup>2+</sup>/Sr<sup>2+</sup> Selectivity in Calcium-Sensing Receptor (CaSR): Implications for Strontium’s Anti-Osteoporosis Effect |
| title_sort |
ca<sup>2+</sup>/sr<sup>2+</sup> selectivity in calcium-sensing receptor (casr): implications for strontium’s anti-osteoporosis effect |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/c7a26232e69549ffba8a8a9ce092b027 |
| work_keys_str_mv |
AT dianacheshmedzhieva casup2supsrsup2supselectivityincalciumsensingreceptorcasrimplicationsforstrontiumsantiosteoporosiseffect AT soniailieva casup2supsrsup2supselectivityincalciumsensingreceptorcasrimplicationsforstrontiumsantiosteoporosiseffect AT eugeneapermyakov casup2supsrsup2supselectivityincalciumsensingreceptorcasrimplicationsforstrontiumsantiosteoporosiseffect AT sergeiepermyakov casup2supsrsup2supselectivityincalciumsensingreceptorcasrimplicationsforstrontiumsantiosteoporosiseffect AT todordudev casup2supsrsup2supselectivityincalciumsensingreceptorcasrimplicationsforstrontiumsantiosteoporosiseffect |
| _version_ |
1718412922319798272 |