The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs
Abstract Face masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications, including at-home testing, but information is needed about whet...
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Nature Portfolio
2021
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oai:doaj.org-article:c7a37bd15abe4d6ea9dd1420686639702021-12-02T14:33:51ZThe SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs10.1038/s41598-021-92665-32045-2322https://doaj.org/article/c7a37bd15abe4d6ea9dd1420686639702021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92665-3https://doaj.org/toc/2045-2322Abstract Face masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications, including at-home testing, but information is needed about whether filters are suited to capture viral particles for SARS-CoV-2 detection. In this study, we generated inactivated virus-laden aerosols of 0.3–2 microns in diameter (0.9 µm mean diameter by mass) and dispersed the aerosolized viral particles onto electrostatic face mask filters. The limit of detection for inactivated coronaviruses SARS-CoV-2 and HCoV-NL63 extracted from filters was between 10 to 100 copies/filter for both viruses. Testing for SARS-CoV-2, using face mask filters and nasopharyngeal swabs collected from hospitalized COVID-19-patients, showed that filter samples offered reduced sensitivity (8.5% compared to nasopharyngeal swabs). The low concordance of SARS-CoV-2 detection between filters and nasopharyngeal swabs indicated that number of viral particles collected on the face mask filter was below the limit of detection for all patients but those with the highest viral loads. This indicated face masks are unsuitable to replace diagnostic nasopharyngeal swabs in COVID-19 diagnosis. The ability to detect nucleic acids on face mask filters may, however, find other uses worth future investigation.Agnieszka SmolinskaDavid S. JessopKirk L. PappanAlexandra De SaedeleerAmerjit KangAlexandra L. MartinMax AllsworthCharlotte TysonMartine P. BosMatt ClancyMike MorelTony CookeTom DymondClaire HarrisJacqui GallowayPaul BresserNynke DijkstraViresh JagesarPaul H. M. SavelkoulErik V. H. BeukenWesley H. V. NixRenaud LouisMuriel DelvauxDoriane CalmesBenoit ErnstSimona PolliniAnna PeiredJulien GuiotSara TomassettiAndries E. BuddingFrank McCaughanStefan J. MarciniakMarc P. van der ScheeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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DOAJ |
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DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Agnieszka Smolinska David S. Jessop Kirk L. Pappan Alexandra De Saedeleer Amerjit Kang Alexandra L. Martin Max Allsworth Charlotte Tyson Martine P. Bos Matt Clancy Mike Morel Tony Cooke Tom Dymond Claire Harris Jacqui Galloway Paul Bresser Nynke Dijkstra Viresh Jagesar Paul H. M. Savelkoul Erik V. H. Beuken Wesley H. V. Nix Renaud Louis Muriel Delvaux Doriane Calmes Benoit Ernst Simona Pollini Anna Peired Julien Guiot Sara Tomassetti Andries E. Budding Frank McCaughan Stefan J. Marciniak Marc P. van der Schee The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| description |
Abstract Face masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications, including at-home testing, but information is needed about whether filters are suited to capture viral particles for SARS-CoV-2 detection. In this study, we generated inactivated virus-laden aerosols of 0.3–2 microns in diameter (0.9 µm mean diameter by mass) and dispersed the aerosolized viral particles onto electrostatic face mask filters. The limit of detection for inactivated coronaviruses SARS-CoV-2 and HCoV-NL63 extracted from filters was between 10 to 100 copies/filter for both viruses. Testing for SARS-CoV-2, using face mask filters and nasopharyngeal swabs collected from hospitalized COVID-19-patients, showed that filter samples offered reduced sensitivity (8.5% compared to nasopharyngeal swabs). The low concordance of SARS-CoV-2 detection between filters and nasopharyngeal swabs indicated that number of viral particles collected on the face mask filter was below the limit of detection for all patients but those with the highest viral loads. This indicated face masks are unsuitable to replace diagnostic nasopharyngeal swabs in COVID-19 diagnosis. The ability to detect nucleic acids on face mask filters may, however, find other uses worth future investigation. |
| format |
article |
| author |
Agnieszka Smolinska David S. Jessop Kirk L. Pappan Alexandra De Saedeleer Amerjit Kang Alexandra L. Martin Max Allsworth Charlotte Tyson Martine P. Bos Matt Clancy Mike Morel Tony Cooke Tom Dymond Claire Harris Jacqui Galloway Paul Bresser Nynke Dijkstra Viresh Jagesar Paul H. M. Savelkoul Erik V. H. Beuken Wesley H. V. Nix Renaud Louis Muriel Delvaux Doriane Calmes Benoit Ernst Simona Pollini Anna Peired Julien Guiot Sara Tomassetti Andries E. Budding Frank McCaughan Stefan J. Marciniak Marc P. van der Schee |
| author_facet |
Agnieszka Smolinska David S. Jessop Kirk L. Pappan Alexandra De Saedeleer Amerjit Kang Alexandra L. Martin Max Allsworth Charlotte Tyson Martine P. Bos Matt Clancy Mike Morel Tony Cooke Tom Dymond Claire Harris Jacqui Galloway Paul Bresser Nynke Dijkstra Viresh Jagesar Paul H. M. Savelkoul Erik V. H. Beuken Wesley H. V. Nix Renaud Louis Muriel Delvaux Doriane Calmes Benoit Ernst Simona Pollini Anna Peired Julien Guiot Sara Tomassetti Andries E. Budding Frank McCaughan Stefan J. Marciniak Marc P. van der Schee |
| author_sort |
Agnieszka Smolinska |
| title |
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| title_short |
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| title_full |
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| title_fullStr |
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| title_full_unstemmed |
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| title_sort |
sars-cov-2 viral load in covid-19 patients is lower on face mask filters than on nasopharyngeal swabs |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c7a37bd15abe4d6ea9dd142068663970 |
| work_keys_str_mv |
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