Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy

Conventional dark-field digital holographic microscopy (DHM) techniques require the use of specialized optics, and, thus, obtaining dark-field images with high contrast has a high cost. Herein, we propose a DHM system that uses an interference-dark-field technique for improving image contrast. Unlik...

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Autores principales:	Chi-Ching Chang, Yang-Kun Chew, Huang-Tian Chan, Mei-Fang Chou, Je-Chung Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	digital holographic microscopy dark-field microscopy interference-dark-field Applied optics. Photonics TA1501-1820
Acceso en línea:	https://doaj.org/article/c7a5910f038b4826a4423d7a8fb38d94
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spelling	oai:doaj.org-article:c7a5910f038b4826a4423d7a8fb38d942021-11-25T18:43:47ZImage Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy10.3390/photonics81105172304-6732https://doaj.org/article/c7a5910f038b4826a4423d7a8fb38d942021-11-01T00:00:00Zhttps://www.mdpi.com/2304-6732/8/11/517https://doaj.org/toc/2304-6732Conventional dark-field digital holographic microscopy (DHM) techniques require the use of specialized optics, and, thus, obtaining dark-field images with high contrast has a high cost. Herein, we propose a DHM system that uses an interference-dark-field technique for improving image contrast. Unlike conventional dark-field DHM, the proposed technique does not require expensive and specialized optical elements, or a complicated optical setup, to obtain dark-field images. The proposed technique employs a pure optical basis method to suppress scattering noise—namely, interference-dark-field—and mainly adopts an arbitrary micro-phase shifting method to achieve destructive interference for obtaining holograms. Under the framework of the proposed technique and through the observation of the USAF 1951 resolution target, the reconstructed image can retain the high contrast of the interference-dark-field DHM. The image contrast is enhanced by at least 43% compared to that which is obtained by conventional dark-field DHM. The resolution of the system can be as high as 0.87 μm. The proposed technique can switch between bright-field and dark-field DHM and prevents damage to the sample, which results from high-intensity illumination in conventional techniques.Chi-Ching ChangYang-Kun ChewHuang-Tian ChanMei-Fang ChouJe-Chung WangMDPI AGarticledigital holographic microscopydark-field microscopyinterference-dark-fieldApplied optics. PhotonicsTA1501-1820ENPhotonics, Vol 8, Iss 517, p 517 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	digital holographic microscopy dark-field microscopy interference-dark-field Applied optics. Photonics TA1501-1820
spellingShingle	digital holographic microscopy dark-field microscopy interference-dark-field Applied optics. Photonics TA1501-1820 Chi-Ching Chang Yang-Kun Chew Huang-Tian Chan Mei-Fang Chou Je-Chung Wang Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy
description	Conventional dark-field digital holographic microscopy (DHM) techniques require the use of specialized optics, and, thus, obtaining dark-field images with high contrast has a high cost. Herein, we propose a DHM system that uses an interference-dark-field technique for improving image contrast. Unlike conventional dark-field DHM, the proposed technique does not require expensive and specialized optical elements, or a complicated optical setup, to obtain dark-field images. The proposed technique employs a pure optical basis method to suppress scattering noise—namely, interference-dark-field—and mainly adopts an arbitrary micro-phase shifting method to achieve destructive interference for obtaining holograms. Under the framework of the proposed technique and through the observation of the USAF 1951 resolution target, the reconstructed image can retain the high contrast of the interference-dark-field DHM. The image contrast is enhanced by at least 43% compared to that which is obtained by conventional dark-field DHM. The resolution of the system can be as high as 0.87 μm. The proposed technique can switch between bright-field and dark-field DHM and prevents damage to the sample, which results from high-intensity illumination in conventional techniques.
format	article
author	Chi-Ching Chang Yang-Kun Chew Huang-Tian Chan Mei-Fang Chou Je-Chung Wang
author_facet	Chi-Ching Chang Yang-Kun Chew Huang-Tian Chan Mei-Fang Chou Je-Chung Wang
author_sort	Chi-Ching Chang
title	Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy
title_short	Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy
title_full	Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy
title_fullStr	Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy
title_full_unstemmed	Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy
title_sort	image contrast improvement in interference-dark-field digital holographic microscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c7a5910f038b4826a4423d7a8fb38d94
work_keys_str_mv	AT chichingchang imagecontrastimprovementininterferencedarkfielddigitalholographicmicroscopy AT yangkunchew imagecontrastimprovementininterferencedarkfielddigitalholographicmicroscopy AT huangtianchan imagecontrastimprovementininterferencedarkfielddigitalholographicmicroscopy AT meifangchou imagecontrastimprovementininterferencedarkfielddigitalholographicmicroscopy AT jechungwang imagecontrastimprovementininterferencedarkfielddigitalholographicmicroscopy
_version_	1718410799043575808

Image Contrast Improvement in Interference-Dark-Field Digital Holographic Microscopy

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