Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.

<h4>Purpose</h4>We examined circulating miRNA expression profiles in plasma of patients with coronary artery disease (CAD) vs. matched controls, with the aim of identifying novel discriminating biomarkers of Stable (SA) and Unstable (UA) angina.<h4>Methods</h4>An exploratory...

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Autores principales:	Yuri D'Alessandra, Maria Cristina Carena, Liana Spazzafumo, Federico Martinelli, Beatrice Bassetti, Paolo Devanna, Mara Rubino, Giancarlo Marenzi, Gualtiero I Colombo, Felice Achilli, Stefano Maggiolini, Maurizio C Capogrossi, Giulio Pompilio
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7a7eb5c8d324fb580773eaad547509a
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spelling	oai:doaj.org-article:c7a7eb5c8d324fb580773eaad547509a2021-11-18T08:46:13ZDiagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.1932-620310.1371/journal.pone.0080345https://doaj.org/article/c7a7eb5c8d324fb580773eaad547509a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260372/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>We examined circulating miRNA expression profiles in plasma of patients with coronary artery disease (CAD) vs. matched controls, with the aim of identifying novel discriminating biomarkers of Stable (SA) and Unstable (UA) angina.<h4>Methods</h4>An exploratory analysis of plasmatic expression profile of 367 miRNAs was conducted in a group of SA and UA patients and control donors, using TaqMan microRNA Arrays. Screening confirmation and expression analysis were performed by qRT-PCR: all miRNAs found dysregulated were examined in the plasma of troponin-negative UA (n=19) and SA (n=34) patients and control subjects (n=20), matched for sex, age, and cardiovascular risk factors. In addition, the expression of 14 known CAD-associated miRNAs was also investigated.<h4>Results</h4>Out of 178 miRNAs consistently detected in plasma samples, 3 showed positive modulation by CAD when compared to controls: miR-337-5p, miR-433, and miR-485-3p. Further, miR-1, -122, -126, -133a, -133b, and miR-199a were positively modulated in both UA and SA patients, while miR-337-5p and miR-145 showed a positive modulation only in SA or UA patients, respectively. ROC curve analyses showed a good diagnostic potential (AUC ≥ 0.85) for miR-1, -126, and -483-5p in SA and for miR-1, -126, and -133a in UA patients vs. controls, respectively. No discriminating AUC values were observed comparing SA vs. UA patients. Hierarchical cluster analysis showed that the combination of miR-1, -133a, and -126 in UA and of miR-1, -126, and -485-3p in SA correctly classified patients vs. controls with an efficiency ≥ 87%. No combination of miRNAs was able to reliably discriminate patients with UA from patients with SA.<h4>Conclusions</h4>This work showed that specific plasmatic miRNA signatures have the potential to accurately discriminate patients with angiographically documented CAD from matched controls. We failed to identify a plasmatic miRNA expression pattern capable to differentiate SA from UA patients.Yuri D'AlessandraMaria Cristina CarenaLiana SpazzafumoFederico MartinelliBeatrice BassettiPaolo DevannaMara RubinoGiancarlo MarenziGualtiero I ColomboFelice AchilliStefano MaggioliniMaurizio C CapogrossiGiulio PompilioPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80345 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuri D'Alessandra Maria Cristina Carena Liana Spazzafumo Federico Martinelli Beatrice Bassetti Paolo Devanna Mara Rubino Giancarlo Marenzi Gualtiero I Colombo Felice Achilli Stefano Maggiolini Maurizio C Capogrossi Giulio Pompilio Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.
description	<h4>Purpose</h4>We examined circulating miRNA expression profiles in plasma of patients with coronary artery disease (CAD) vs. matched controls, with the aim of identifying novel discriminating biomarkers of Stable (SA) and Unstable (UA) angina.<h4>Methods</h4>An exploratory analysis of plasmatic expression profile of 367 miRNAs was conducted in a group of SA and UA patients and control donors, using TaqMan microRNA Arrays. Screening confirmation and expression analysis were performed by qRT-PCR: all miRNAs found dysregulated were examined in the plasma of troponin-negative UA (n=19) and SA (n=34) patients and control subjects (n=20), matched for sex, age, and cardiovascular risk factors. In addition, the expression of 14 known CAD-associated miRNAs was also investigated.<h4>Results</h4>Out of 178 miRNAs consistently detected in plasma samples, 3 showed positive modulation by CAD when compared to controls: miR-337-5p, miR-433, and miR-485-3p. Further, miR-1, -122, -126, -133a, -133b, and miR-199a were positively modulated in both UA and SA patients, while miR-337-5p and miR-145 showed a positive modulation only in SA or UA patients, respectively. ROC curve analyses showed a good diagnostic potential (AUC ≥ 0.85) for miR-1, -126, and -483-5p in SA and for miR-1, -126, and -133a in UA patients vs. controls, respectively. No discriminating AUC values were observed comparing SA vs. UA patients. Hierarchical cluster analysis showed that the combination of miR-1, -133a, and -126 in UA and of miR-1, -126, and -485-3p in SA correctly classified patients vs. controls with an efficiency ≥ 87%. No combination of miRNAs was able to reliably discriminate patients with UA from patients with SA.<h4>Conclusions</h4>This work showed that specific plasmatic miRNA signatures have the potential to accurately discriminate patients with angiographically documented CAD from matched controls. We failed to identify a plasmatic miRNA expression pattern capable to differentiate SA from UA patients.
format	article
author	Yuri D'Alessandra Maria Cristina Carena Liana Spazzafumo Federico Martinelli Beatrice Bassetti Paolo Devanna Mara Rubino Giancarlo Marenzi Gualtiero I Colombo Felice Achilli Stefano Maggiolini Maurizio C Capogrossi Giulio Pompilio
author_facet	Yuri D'Alessandra Maria Cristina Carena Liana Spazzafumo Federico Martinelli Beatrice Bassetti Paolo Devanna Mara Rubino Giancarlo Marenzi Gualtiero I Colombo Felice Achilli Stefano Maggiolini Maurizio C Capogrossi Giulio Pompilio
author_sort	Yuri D'Alessandra
title	Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.
title_short	Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.
title_full	Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.
title_fullStr	Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.
title_full_unstemmed	Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.
title_sort	diagnostic potential of plasmatic microrna signatures in stable and unstable angina.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c7a7eb5c8d324fb580773eaad547509a
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Diagnostic potential of plasmatic MicroRNA signatures in stable and unstable angina.

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