ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.

Laser scanning confocal microscopy has become an invaluable tool in biomedical research but regular quality testing is vital to maintain the system's performance for diagnostic and research purposes. Although many methods have been devised over the years to characterise specific aspects of a co...

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Autores principales:	Keng Imm Hng, Dirk Dormann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7aa3414e9484888b1620fee658daacf
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spelling	oai:doaj.org-article:c7aa3414e9484888b1620fee658daacf2021-11-18T08:48:15ZConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.1932-620310.1371/journal.pone.0079879https://doaj.org/article/c7aa3414e9484888b1620fee658daacf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24224017/?tool=EBIhttps://doaj.org/toc/1932-6203Laser scanning confocal microscopy has become an invaluable tool in biomedical research but regular quality testing is vital to maintain the system's performance for diagnostic and research purposes. Although many methods have been devised over the years to characterise specific aspects of a confocal microscope like measuring the optical point spread function or the field illumination, only very few analysis tools are available. Our aim was to develop a comprehensive quality assurance framework ranging from image acquisition to automated analysis and documentation. We created standardised test data to assess the performance of the lasers, the objective lenses and other key components required for optimum confocal operation. The ConfocalCheck software presented here analyses the data fully automatically. It creates numerous visual outputs indicating potential issues requiring further investigation. By storing results in a web browser compatible file format the software greatly simplifies record keeping allowing the operator to quickly compare old and new data and to spot developing trends. We demonstrate that the systematic monitoring of confocal performance is essential in a core facility environment and how the quantitative measurements obtained can be used for the detailed characterisation of system components as well as for comparisons across multiple instruments.Keng Imm HngDirk DormannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79879 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keng Imm Hng Dirk Dormann ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.
description	Laser scanning confocal microscopy has become an invaluable tool in biomedical research but regular quality testing is vital to maintain the system's performance for diagnostic and research purposes. Although many methods have been devised over the years to characterise specific aspects of a confocal microscope like measuring the optical point spread function or the field illumination, only very few analysis tools are available. Our aim was to develop a comprehensive quality assurance framework ranging from image acquisition to automated analysis and documentation. We created standardised test data to assess the performance of the lasers, the objective lenses and other key components required for optimum confocal operation. The ConfocalCheck software presented here analyses the data fully automatically. It creates numerous visual outputs indicating potential issues requiring further investigation. By storing results in a web browser compatible file format the software greatly simplifies record keeping allowing the operator to quickly compare old and new data and to spot developing trends. We demonstrate that the systematic monitoring of confocal performance is essential in a core facility environment and how the quantitative measurements obtained can be used for the detailed characterisation of system components as well as for comparisons across multiple instruments.
format	article
author	Keng Imm Hng Dirk Dormann
author_facet	Keng Imm Hng Dirk Dormann
author_sort	Keng Imm Hng
title	ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.
title_short	ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.
title_full	ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.
title_fullStr	ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.
title_full_unstemmed	ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.
title_sort	confocalcheck--a software tool for the automated monitoring of confocal microscope performance.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c7aa3414e9484888b1620fee658daacf
work_keys_str_mv	AT kengimmhng confocalcheckasoftwaretoolfortheautomatedmonitoringofconfocalmicroscopeperformance AT dirkdormann confocalcheckasoftwaretoolfortheautomatedmonitoringofconfocalmicroscopeperformance
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ConfocalCheck--a software tool for the automated monitoring of confocal microscope performance.

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