The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection

ABSTRACT The mechanism(s) by which Lactobacillus-dominated cervicovaginal microbiota provide a barrier to Chlamydia trachomatis infection remain(s) unknown. Here we evaluate the impact of different Lactobacillus spp. identified via culture-independent metataxonomic analysis of C. trachomatis-infecte...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vonetta L. Edwards, Steven B. Smith, Elias J. McComb, Jeanne Tamarelle, Bing Ma, Michael S. Humphrys, Pawel Gajer, Kathleen Gwilliam, Alison M. Schaefer, Samuel K. Lai, Mishka Terplan, Katrina S. Mark, Rebecca M. Brotman, Larry J. Forney, Patrik M. Bavoil, Jacques Ravel
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Lactobacillus epigenetic lactic acid microbiome proliferation sexually transmitted infection Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c7ac43892b7246beabc1cea4bec10970
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c7ac43892b7246beabc1cea4bec10970
record_format	dspace
spelling	oai:doaj.org-article:c7ac43892b7246beabc1cea4bec109702021-11-15T16:22:10ZThe Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection10.1128/mBio.01548-192150-7511https://doaj.org/article/c7ac43892b7246beabc1cea4bec109702019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01548-19https://doaj.org/toc/2150-7511ABSTRACT The mechanism(s) by which Lactobacillus-dominated cervicovaginal microbiota provide a barrier to Chlamydia trachomatis infection remain(s) unknown. Here we evaluate the impact of different Lactobacillus spp. identified via culture-independent metataxonomic analysis of C. trachomatis-infected women on C. trachomatis infection in a three-dimensional (3D) cervical epithelium model. Lactobacillus spp. that specifically produce d(−) lactic acid were associated with long-term protection against C. trachomatis infection, consistent with reduced protection associated with Lactobacillus iners, which does not produce this isoform, and with decreased epithelial cell proliferation, consistent with the observed prolonged protective effect. Transcriptomic analysis revealed that epigenetic modifications involving histone deacetylase-controlled pathways are integral to the cross talk between host and microbiota. These results highlight a fundamental mechanism whereby the cervicovaginal microbiota modulates host functions to protect against C. trachomatis infection. IMPORTANCE The vaginal microbiota is believed to protect women against Chlamydia trachomatis, the etiologic agent of the most prevalent sexually transmitted infection (STI) in developed countries. The mechanism underlying this protection has remained elusive. Here, we reveal the comprehensive strategy by which the cervicovaginal microbiota modulates host functions to protect against chlamydial infection, thereby providing a novel conceptual mechanistic understanding. Major implications of this work are that (i) the impact of the vaginal microbiota on the epithelium should be considered in future studies of chlamydial infection and other STIs and (ii) a fundamental understanding of the cervicovaginal microbiota’s role in protection against STIs may enable the development of novel microbiome-based therapeutic strategies to protect women from infection and improve vaginal and cervical health.Vonetta L. EdwardsSteven B. SmithElias J. McCombJeanne TamarelleBing MaMichael S. HumphrysPawel GajerKathleen GwilliamAlison M. SchaeferSamuel K. LaiMishka TerplanKatrina S. MarkRebecca M. BrotmanLarry J. ForneyPatrik M. BavoilJacques RavelAmerican Society for MicrobiologyarticleLactobacillusepigeneticlactic acidmicrobiomeproliferationsexually transmitted infectionMicrobiologyQR1-502ENmBio, Vol 10, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lactobacillus epigenetic lactic acid microbiome proliferation sexually transmitted infection Microbiology QR1-502
spellingShingle	Lactobacillus epigenetic lactic acid microbiome proliferation sexually transmitted infection Microbiology QR1-502 Vonetta L. Edwards Steven B. Smith Elias J. McComb Jeanne Tamarelle Bing Ma Michael S. Humphrys Pawel Gajer Kathleen Gwilliam Alison M. Schaefer Samuel K. Lai Mishka Terplan Katrina S. Mark Rebecca M. Brotman Larry J. Forney Patrik M. Bavoil Jacques Ravel The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection
description	ABSTRACT The mechanism(s) by which Lactobacillus-dominated cervicovaginal microbiota provide a barrier to Chlamydia trachomatis infection remain(s) unknown. Here we evaluate the impact of different Lactobacillus spp. identified via culture-independent metataxonomic analysis of C. trachomatis-infected women on C. trachomatis infection in a three-dimensional (3D) cervical epithelium model. Lactobacillus spp. that specifically produce d(−) lactic acid were associated with long-term protection against C. trachomatis infection, consistent with reduced protection associated with Lactobacillus iners, which does not produce this isoform, and with decreased epithelial cell proliferation, consistent with the observed prolonged protective effect. Transcriptomic analysis revealed that epigenetic modifications involving histone deacetylase-controlled pathways are integral to the cross talk between host and microbiota. These results highlight a fundamental mechanism whereby the cervicovaginal microbiota modulates host functions to protect against C. trachomatis infection. IMPORTANCE The vaginal microbiota is believed to protect women against Chlamydia trachomatis, the etiologic agent of the most prevalent sexually transmitted infection (STI) in developed countries. The mechanism underlying this protection has remained elusive. Here, we reveal the comprehensive strategy by which the cervicovaginal microbiota modulates host functions to protect against chlamydial infection, thereby providing a novel conceptual mechanistic understanding. Major implications of this work are that (i) the impact of the vaginal microbiota on the epithelium should be considered in future studies of chlamydial infection and other STIs and (ii) a fundamental understanding of the cervicovaginal microbiota’s role in protection against STIs may enable the development of novel microbiome-based therapeutic strategies to protect women from infection and improve vaginal and cervical health.
format	article
author	Vonetta L. Edwards Steven B. Smith Elias J. McComb Jeanne Tamarelle Bing Ma Michael S. Humphrys Pawel Gajer Kathleen Gwilliam Alison M. Schaefer Samuel K. Lai Mishka Terplan Katrina S. Mark Rebecca M. Brotman Larry J. Forney Patrik M. Bavoil Jacques Ravel
author_facet	Vonetta L. Edwards Steven B. Smith Elias J. McComb Jeanne Tamarelle Bing Ma Michael S. Humphrys Pawel Gajer Kathleen Gwilliam Alison M. Schaefer Samuel K. Lai Mishka Terplan Katrina S. Mark Rebecca M. Brotman Larry J. Forney Patrik M. Bavoil Jacques Ravel
author_sort	Vonetta L. Edwards
title	The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection
title_short	The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection
title_full	The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection
title_fullStr	The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection
title_full_unstemmed	The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection
title_sort	cervicovaginal microbiota-host interaction modulates <named-content content-type="genus-species">chlamydia trachomatis</named-content> infection
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/c7ac43892b7246beabc1cea4bec10970
work_keys_str_mv	AT vonettaledwards thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT stevenbsmith thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT eliasjmccomb thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT jeannetamarelle thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT bingma thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT michaelshumphrys thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT pawelgajer thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT kathleengwilliam thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT alisonmschaefer thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT samuelklai thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT mishkaterplan thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT katrinasmark thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT rebeccambrotman thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT larryjforney thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT patrikmbavoil thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT jacquesravel thecervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT vonettaledwards cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT stevenbsmith cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT eliasjmccomb cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT jeannetamarelle cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT bingma cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT michaelshumphrys cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT pawelgajer cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT kathleengwilliam cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT alisonmschaefer cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT samuelklai cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT mishkaterplan cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT katrinasmark cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT rebeccambrotman cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT larryjforney cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT patrikmbavoil cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection AT jacquesravel cervicovaginalmicrobiotahostinteractionmodulatesnamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentinfection
_version_	1718426931294109696

The Cervicovaginal Microbiota-Host Interaction Modulates <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Infection

Ejemplares similares