AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.

<h4>Background</h4>AGO1 associates with microRNAs (miRNAs) and regulates mRNAs through cleavage and translational repression. AGO1 homeostasis entails DCL1-dependent production of miR168 from MIR168a and MIR168b transcripts, post-transcriptional stabilization of miR168 by AGO1, and AGO1-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Hervé Vaucheret
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7b938bf16c345b1a5dcca673fb121d9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c7b938bf16c345b1a5dcca673fb121d9
record_format	dspace
spelling	oai:doaj.org-article:c7b938bf16c345b1a5dcca673fb121d92021-11-25T06:21:19ZAGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.1932-620310.1371/journal.pone.0006442https://doaj.org/article/c7b938bf16c345b1a5dcca673fb121d92009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19649244/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>AGO1 associates with microRNAs (miRNAs) and regulates mRNAs through cleavage and translational repression. AGO1 homeostasis entails DCL1-dependent production of miR168 from MIR168a and MIR168b transcripts, post-transcriptional stabilization of miR168 by AGO1, and AGO1-catalyzed miR168-guided cleavage of AGO1 mRNA.<h4>Principal findings</h4>This study reveals that MIR168a is highly expressed and predominantly produces a 21-nt miR168 species. By contrast, MIR168b is expressed at low levels and produces an equal amount of 21- and 22-nt miR168 species. Only the 21-nt miR168 is preferentially stabilized by AGO1, and consequently, the accumulation of the 22-nt but not the 21-nt miR168 is reduced when DCL1 activity is impaired. mir168a mutants with strongly reduced levels of 21-nt miR168 are viable but exhibit developmental defects, particularly during environmentally challenging conditions.<h4>Conclusions/significance</h4>These results suggest that 22-nt miR168 ensures basal cleavage of AGO1 mRNA whereas 21-nt miR168 permits an effective response to endogenous or environmental fluctuations owing to its preferential stabilization by AGO1.Hervé VaucheretPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6442 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hervé Vaucheret AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.
description	<h4>Background</h4>AGO1 associates with microRNAs (miRNAs) and regulates mRNAs through cleavage and translational repression. AGO1 homeostasis entails DCL1-dependent production of miR168 from MIR168a and MIR168b transcripts, post-transcriptional stabilization of miR168 by AGO1, and AGO1-catalyzed miR168-guided cleavage of AGO1 mRNA.<h4>Principal findings</h4>This study reveals that MIR168a is highly expressed and predominantly produces a 21-nt miR168 species. By contrast, MIR168b is expressed at low levels and produces an equal amount of 21- and 22-nt miR168 species. Only the 21-nt miR168 is preferentially stabilized by AGO1, and consequently, the accumulation of the 22-nt but not the 21-nt miR168 is reduced when DCL1 activity is impaired. mir168a mutants with strongly reduced levels of 21-nt miR168 are viable but exhibit developmental defects, particularly during environmentally challenging conditions.<h4>Conclusions/significance</h4>These results suggest that 22-nt miR168 ensures basal cleavage of AGO1 mRNA whereas 21-nt miR168 permits an effective response to endogenous or environmental fluctuations owing to its preferential stabilization by AGO1.
format	article
author	Hervé Vaucheret
author_facet	Hervé Vaucheret
author_sort	Hervé Vaucheret
title	AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.
title_short	AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.
title_full	AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.
title_fullStr	AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.
title_full_unstemmed	AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.
title_sort	ago1 homeostasis involves differential production of 21-nt and 22-nt mir168 species by mir168a and mir168b.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/c7b938bf16c345b1a5dcca673fb121d9
work_keys_str_mv	AT hervevaucheret ago1homeostasisinvolvesdifferentialproductionof21ntand22ntmir168speciesbymir168aandmir168b
_version_	1718413826128347136

AGO1 homeostasis involves differential production of 21-nt and 22-nt miR168 species by MIR168a and MIR168b.

Ejemplares similares