Stress reaction in outer segments of photoreceptors after blue light irradiation.

The retina is prone to oxidative stress from many factors which are also involved in the pathogenesis of degenerative diseases. In this study, we used the application of blue light as a physiological stress factor. The aim of this study was to identify the major source of intracellular ROS that medi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cora Roehlecke, Ulrike Schumann, Marius Ader, Coy Brunssen, Silvia Bramke, Henning Morawietz, Richard H W Funk
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7ba61db9629419e87f8083141b47299
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c7ba61db9629419e87f8083141b47299
record_format	dspace
spelling	oai:doaj.org-article:c7ba61db9629419e87f8083141b472992021-11-18T08:55:51ZStress reaction in outer segments of photoreceptors after blue light irradiation.1932-620310.1371/journal.pone.0071570https://doaj.org/article/c7ba61db9629419e87f8083141b472992013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24039718/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The retina is prone to oxidative stress from many factors which are also involved in the pathogenesis of degenerative diseases. In this study, we used the application of blue light as a physiological stress factor. The aim of this study was to identify the major source of intracellular ROS that mediates blue light-induced detrimental effects on cells which may lead to cytotoxicity. We hypothesized that outer segments are the major source of blue light induced ROS generation. In photoreceptors, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) enzymes and the recently found respiratory chain complexes may represent a major source for reactive oxygen species (ROS), beside mitochondria and chromophores. Therefore, we investigated this hypothesis and analysed the exact localization of the ROS source in photoreceptors in an organotypic culture system for mouse retinas. Whole eyeball cultures were irradiated with visible blue light (405 nm) with an output power of 1 mW/cm². Blue light impingement lead to an increase of ROS production (detected by H2DCFDA in live retinal explants), which was particularly strong in the photoreceptor outer segments. Nox-2 and Nox-4 proteins are sources of ROS in blue light irradiated photoreceptors; the Nox inhibitor apocynin decreased ROS stimulated by blue light. Concomitantly, enzyme SOD-1, a member of the antioxidant defense system, indicator molecules of protein oxidation (CML) and lipid oxidation (MDA and 4-HNE) were also increased in the outer segments. Interestingly, outer segments showed a mitochondrial-like membrane potential which was demonstrated using two dyes (JC-1 and TMRE) normally exclusively associated with mitochondria. As in mitochondria, these dyes indicated a decrease of the membrane potential in hypoxic states or cell stress situations. The present study demonstrates that ROS generation and oxidative stress occurs directly in the outer segments of photoreceptors after blue light irradiation.Cora RoehleckeUlrike SchumannMarius AderCoy BrunssenSilvia BramkeHenning MorawietzRichard H W FunkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e71570 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cora Roehlecke Ulrike Schumann Marius Ader Coy Brunssen Silvia Bramke Henning Morawietz Richard H W Funk Stress reaction in outer segments of photoreceptors after blue light irradiation.
description	The retina is prone to oxidative stress from many factors which are also involved in the pathogenesis of degenerative diseases. In this study, we used the application of blue light as a physiological stress factor. The aim of this study was to identify the major source of intracellular ROS that mediates blue light-induced detrimental effects on cells which may lead to cytotoxicity. We hypothesized that outer segments are the major source of blue light induced ROS generation. In photoreceptors, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) enzymes and the recently found respiratory chain complexes may represent a major source for reactive oxygen species (ROS), beside mitochondria and chromophores. Therefore, we investigated this hypothesis and analysed the exact localization of the ROS source in photoreceptors in an organotypic culture system for mouse retinas. Whole eyeball cultures were irradiated with visible blue light (405 nm) with an output power of 1 mW/cm². Blue light impingement lead to an increase of ROS production (detected by H2DCFDA in live retinal explants), which was particularly strong in the photoreceptor outer segments. Nox-2 and Nox-4 proteins are sources of ROS in blue light irradiated photoreceptors; the Nox inhibitor apocynin decreased ROS stimulated by blue light. Concomitantly, enzyme SOD-1, a member of the antioxidant defense system, indicator molecules of protein oxidation (CML) and lipid oxidation (MDA and 4-HNE) were also increased in the outer segments. Interestingly, outer segments showed a mitochondrial-like membrane potential which was demonstrated using two dyes (JC-1 and TMRE) normally exclusively associated with mitochondria. As in mitochondria, these dyes indicated a decrease of the membrane potential in hypoxic states or cell stress situations. The present study demonstrates that ROS generation and oxidative stress occurs directly in the outer segments of photoreceptors after blue light irradiation.
format	article
author	Cora Roehlecke Ulrike Schumann Marius Ader Coy Brunssen Silvia Bramke Henning Morawietz Richard H W Funk
author_facet	Cora Roehlecke Ulrike Schumann Marius Ader Coy Brunssen Silvia Bramke Henning Morawietz Richard H W Funk
author_sort	Cora Roehlecke
title	Stress reaction in outer segments of photoreceptors after blue light irradiation.
title_short	Stress reaction in outer segments of photoreceptors after blue light irradiation.
title_full	Stress reaction in outer segments of photoreceptors after blue light irradiation.
title_fullStr	Stress reaction in outer segments of photoreceptors after blue light irradiation.
title_full_unstemmed	Stress reaction in outer segments of photoreceptors after blue light irradiation.
title_sort	stress reaction in outer segments of photoreceptors after blue light irradiation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c7ba61db9629419e87f8083141b47299
work_keys_str_mv	AT coraroehlecke stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation AT ulrikeschumann stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation AT mariusader stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation AT coybrunssen stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation AT silviabramke stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation AT henningmorawietz stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation AT richardhwfunk stressreactioninoutersegmentsofphotoreceptorsafterbluelightirradiation
_version_	1718421165569998848

Stress reaction in outer segments of photoreceptors after blue light irradiation.

Ejemplares similares