Landscape of Hopx expression in cells of the immune system

Homeodomain only protein (Hopx) is a regulator of cell differentiation and function, and it has also emerged as a crucial marker of specific developmental and differentiation potentials. Hopx expression and functions have been identified in some stem cells, tumors, and in certain immune cells. Howev...

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Autores principales:	Jessica Bourque, Adeleye Opejin, Alexey Surnov, Courtney A. Iberg, Cindy Gross, Rajan Jain, Jonathan A. Epstein, Daniel Hawiger
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Hopx Immune cells scRNA-seq Mice Flow cytometry Expression Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/c7c4ba982f4e448781ed717303b4b161
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spelling	oai:doaj.org-article:c7c4ba982f4e448781ed717303b4b1612021-12-02T05:02:34ZLandscape of Hopx expression in cells of the immune system2405-844010.1016/j.heliyon.2021.e08311https://doaj.org/article/c7c4ba982f4e448781ed717303b4b1612021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021024142https://doaj.org/toc/2405-8440Homeodomain only protein (Hopx) is a regulator of cell differentiation and function, and it has also emerged as a crucial marker of specific developmental and differentiation potentials. Hopx expression and functions have been identified in some stem cells, tumors, and in certain immune cells. However, expression of Hopx in immune cells remains insufficiently characterized. Here we report a comprehensive pattern of Hopx expression in multiple types of immune cells under steady state conditions. By utilizing single-cell RNA sequencing (scRNA-seq) and flow cytometric analysis, we characterize a constitutive expression of Hopx in specific subsets of CD4+ and CD8+ T cells and B cells, as well as natural killer (NK), NKT, and myeloid cells. In contrast, Hopx expression is not present in conventional dendritic cells and eosinophils. The utility of identifying expression of Hopx in immune cells may prove vital in delineating specific roles of Hopx under multiple immune conditions.Jessica BourqueAdeleye OpejinAlexey SurnovCourtney A. IbergCindy GrossRajan JainJonathan A. EpsteinDaniel HawigerElsevierarticleHopxImmune cellsscRNA-seqMiceFlow cytometryExpressionScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08311- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hopx Immune cells scRNA-seq Mice Flow cytometry Expression Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Hopx Immune cells scRNA-seq Mice Flow cytometry Expression Science (General) Q1-390 Social sciences (General) H1-99 Jessica Bourque Adeleye Opejin Alexey Surnov Courtney A. Iberg Cindy Gross Rajan Jain Jonathan A. Epstein Daniel Hawiger Landscape of Hopx expression in cells of the immune system
description	Homeodomain only protein (Hopx) is a regulator of cell differentiation and function, and it has also emerged as a crucial marker of specific developmental and differentiation potentials. Hopx expression and functions have been identified in some stem cells, tumors, and in certain immune cells. However, expression of Hopx in immune cells remains insufficiently characterized. Here we report a comprehensive pattern of Hopx expression in multiple types of immune cells under steady state conditions. By utilizing single-cell RNA sequencing (scRNA-seq) and flow cytometric analysis, we characterize a constitutive expression of Hopx in specific subsets of CD4+ and CD8+ T cells and B cells, as well as natural killer (NK), NKT, and myeloid cells. In contrast, Hopx expression is not present in conventional dendritic cells and eosinophils. The utility of identifying expression of Hopx in immune cells may prove vital in delineating specific roles of Hopx under multiple immune conditions.
format	article
author	Jessica Bourque Adeleye Opejin Alexey Surnov Courtney A. Iberg Cindy Gross Rajan Jain Jonathan A. Epstein Daniel Hawiger
author_facet	Jessica Bourque Adeleye Opejin Alexey Surnov Courtney A. Iberg Cindy Gross Rajan Jain Jonathan A. Epstein Daniel Hawiger
author_sort	Jessica Bourque
title	Landscape of Hopx expression in cells of the immune system
title_short	Landscape of Hopx expression in cells of the immune system
title_full	Landscape of Hopx expression in cells of the immune system
title_fullStr	Landscape of Hopx expression in cells of the immune system
title_full_unstemmed	Landscape of Hopx expression in cells of the immune system
title_sort	landscape of hopx expression in cells of the immune system
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c7c4ba982f4e448781ed717303b4b161
work_keys_str_mv	AT jessicabourque landscapeofhopxexpressionincellsoftheimmunesystem AT adeleyeopejin landscapeofhopxexpressionincellsoftheimmunesystem AT alexeysurnov landscapeofhopxexpressionincellsoftheimmunesystem AT courtneyaiberg landscapeofhopxexpressionincellsoftheimmunesystem AT cindygross landscapeofhopxexpressionincellsoftheimmunesystem AT rajanjain landscapeofhopxexpressionincellsoftheimmunesystem AT jonathanaepstein landscapeofhopxexpressionincellsoftheimmunesystem AT danielhawiger landscapeofhopxexpressionincellsoftheimmunesystem
_version_	1718400736904085504

Landscape of Hopx expression in cells of the immune system

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