Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.

<h4>Background</h4>Production of inflammatory cytokines by mesenteric adipose tissue (MAT) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Animal models of colitis have demonstrated inflammatory changes within MAT, but it is unclear if these changes occur in...

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Autores principales:	W Conan Mustain, Marlene E Starr, Joseph D Valentino, Donald A Cohen, Daiki Okamura, Chi Wang, B Mark Evers, Hiroshi Saito
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7ce3160fd6b434bb6387decf8b4792e
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spelling	oai:doaj.org-article:c7ce3160fd6b434bb6387decf8b4792e2021-11-18T08:40:19ZInflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.1932-620310.1371/journal.pone.0083693https://doaj.org/article/c7ce3160fd6b434bb6387decf8b4792e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386254/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Production of inflammatory cytokines by mesenteric adipose tissue (MAT) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Animal models of colitis have demonstrated inflammatory changes within MAT, but it is unclear if these changes occur in isolation or as part of a systemic adipose tissue response. It is also unknown what cell types are responsible for cytokine production within MAT. The present study was designed to determine whether cytokine production by MAT during experimental colitis is depot-specific, and also to identify the source of cytokine production within MAT.<h4>Methods</h4>Experimental colitis was induced in 6-month-old C57BL/6 mice by administration of dextran sulfate sodium (2% in drinking water) for up to 5 days. The induction of cytokine mRNA within various adipose tissues, including mesenteric, epididymal, and subcutaneous, was analyzed by qRT-PCR. These adipose tissues were also examined for histological evidence of inflammation. The level of cytokine mRNA during acute colitis was compared between mature mesenteric adipocytes, mesenteric stromal vascular fraction (SVF), and mesenteric lymph nodes.<h4>Results</h4>During acute colitis, MAT exhibited an increased presence of infiltrating mononuclear cells and fibrotic structures, as well as decreased adipocyte size. The mRNA levels of TNF-α, IL-1β, and IL-6 were significantly increased in MAT but not other adipose tissue depots. Within the MAT, induction of these cytokines was observed mainly in the SVF.<h4>Conclusions</h4>Acute experimental colitis causes a strong site-specific inflammatory response within MAT, which is mediated by cells of the SVF, rather than mature adipocytes or mesenteric lymph nodes.W Conan MustainMarlene E StarrJoseph D ValentinoDonald A CohenDaiki OkamuraChi WangB Mark EversHiroshi SaitoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83693 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q W Conan Mustain Marlene E Starr Joseph D Valentino Donald A Cohen Daiki Okamura Chi Wang B Mark Evers Hiroshi Saito Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
description	<h4>Background</h4>Production of inflammatory cytokines by mesenteric adipose tissue (MAT) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Animal models of colitis have demonstrated inflammatory changes within MAT, but it is unclear if these changes occur in isolation or as part of a systemic adipose tissue response. It is also unknown what cell types are responsible for cytokine production within MAT. The present study was designed to determine whether cytokine production by MAT during experimental colitis is depot-specific, and also to identify the source of cytokine production within MAT.<h4>Methods</h4>Experimental colitis was induced in 6-month-old C57BL/6 mice by administration of dextran sulfate sodium (2% in drinking water) for up to 5 days. The induction of cytokine mRNA within various adipose tissues, including mesenteric, epididymal, and subcutaneous, was analyzed by qRT-PCR. These adipose tissues were also examined for histological evidence of inflammation. The level of cytokine mRNA during acute colitis was compared between mature mesenteric adipocytes, mesenteric stromal vascular fraction (SVF), and mesenteric lymph nodes.<h4>Results</h4>During acute colitis, MAT exhibited an increased presence of infiltrating mononuclear cells and fibrotic structures, as well as decreased adipocyte size. The mRNA levels of TNF-α, IL-1β, and IL-6 were significantly increased in MAT but not other adipose tissue depots. Within the MAT, induction of these cytokines was observed mainly in the SVF.<h4>Conclusions</h4>Acute experimental colitis causes a strong site-specific inflammatory response within MAT, which is mediated by cells of the SVF, rather than mature adipocytes or mesenteric lymph nodes.
format	article
author	W Conan Mustain Marlene E Starr Joseph D Valentino Donald A Cohen Daiki Okamura Chi Wang B Mark Evers Hiroshi Saito
author_facet	W Conan Mustain Marlene E Starr Joseph D Valentino Donald A Cohen Daiki Okamura Chi Wang B Mark Evers Hiroshi Saito
author_sort	W Conan Mustain
title	Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
title_short	Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
title_full	Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
title_fullStr	Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
title_full_unstemmed	Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
title_sort	inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c7ce3160fd6b434bb6387decf8b4792e
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Inflammatory cytokine gene expression in mesenteric adipose tissue during acute experimental colitis.

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