Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells.
<h4>Introduction</h4>Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear.<h4>Methods</h4>In EPC colony forming assays, we first demonstrated that the formation...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c7cf276af78541099a90d8db8ea9fd54 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c7cf276af78541099a90d8db8ea9fd54 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c7cf276af78541099a90d8db8ea9fd542021-11-25T06:02:46ZCross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells.1932-620310.1371/journal.pone.0106310https://doaj.org/article/c7cf276af78541099a90d8db8ea9fd542014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25166961/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear.<h4>Methods</h4>In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34+ and CD34- cells, and determined the optimal concentrations of CD34+ cells and CD34- cells for spindle-shaped EPC differentiation.<h4>Results</h4>Functionally, the coculture of CD34+ and CD34- cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34+ cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34+ and CD34- cells in a murine hind-limb ischemia model. To elucidate further the role of hematopoietic cells in EPC differentiation, we isolated different populations of hematopoietic cells. T lymphocytes (CD3+) markedly accelerated the early EPC status of CD34+ cells, while macrophages (CD11b+) or megakaryocytes (CD41+) specifically promoted large EPC colonies.<h4>Conclusion</h4>Our results suggest that specific populations of hematopoietic cells play a role in the EPC differentiation of CD34+ cells, a finding that may aid in the development of a novel cell therapy strategy to overcome the quantitative and qualitative limitations of EPC therapy.Sang-Mo KwonJun-Hee LeeSang-Hun LeeSeok-Yun JungDa-Yeon KimSong-Hwa KangSo-Young YooJong-Kyu HongJi-Hye ParkJung-Hee KimSung-Wook KimYeon-Ju KimSun-Jin LeeHwi-Gon KimTakayuki AsaharaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e106310 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Sang-Mo Kwon Jun-Hee Lee Sang-Hun Lee Seok-Yun Jung Da-Yeon Kim Song-Hwa Kang So-Young Yoo Jong-Kyu Hong Ji-Hye Park Jung-Hee Kim Sung-Wook Kim Yeon-Ju Kim Sun-Jin Lee Hwi-Gon Kim Takayuki Asahara Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells. |
| description |
<h4>Introduction</h4>Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear.<h4>Methods</h4>In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34+ and CD34- cells, and determined the optimal concentrations of CD34+ cells and CD34- cells for spindle-shaped EPC differentiation.<h4>Results</h4>Functionally, the coculture of CD34+ and CD34- cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34+ cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34+ and CD34- cells in a murine hind-limb ischemia model. To elucidate further the role of hematopoietic cells in EPC differentiation, we isolated different populations of hematopoietic cells. T lymphocytes (CD3+) markedly accelerated the early EPC status of CD34+ cells, while macrophages (CD11b+) or megakaryocytes (CD41+) specifically promoted large EPC colonies.<h4>Conclusion</h4>Our results suggest that specific populations of hematopoietic cells play a role in the EPC differentiation of CD34+ cells, a finding that may aid in the development of a novel cell therapy strategy to overcome the quantitative and qualitative limitations of EPC therapy. |
| format |
article |
| author |
Sang-Mo Kwon Jun-Hee Lee Sang-Hun Lee Seok-Yun Jung Da-Yeon Kim Song-Hwa Kang So-Young Yoo Jong-Kyu Hong Ji-Hye Park Jung-Hee Kim Sung-Wook Kim Yeon-Ju Kim Sun-Jin Lee Hwi-Gon Kim Takayuki Asahara |
| author_facet |
Sang-Mo Kwon Jun-Hee Lee Sang-Hun Lee Seok-Yun Jung Da-Yeon Kim Song-Hwa Kang So-Young Yoo Jong-Kyu Hong Ji-Hye Park Jung-Hee Kim Sung-Wook Kim Yeon-Ju Kim Sun-Jin Lee Hwi-Gon Kim Takayuki Asahara |
| author_sort |
Sang-Mo Kwon |
| title |
Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells. |
| title_short |
Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells. |
| title_full |
Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells. |
| title_fullStr |
Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells. |
| title_full_unstemmed |
Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells. |
| title_sort |
cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of cd34 positive cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/c7cf276af78541099a90d8db8ea9fd54 |
| work_keys_str_mv |
AT sangmokwon crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT junheelee crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT sanghunlee crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT seokyunjung crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT dayeonkim crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT songhwakang crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT soyoungyoo crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT jongkyuhong crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT jihyepark crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT jungheekim crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT sungwookkim crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT yeonjukim crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT sunjinlee crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT hwigonkim crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells AT takayukiasahara crosstalkwithhematopoieticcellsregulatestheendothelialprogenitorcelldifferentiationofcd34positivecells |
| _version_ |
1718414247598227456 |