Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan

Abstract In mammals, peripheral serotonin is involved in regulating energy balance. Herein, we characterized the transcriptomic profile and microstructure of adipose and muscle in pre-weaned calves with increased circulating serotonin. Holstein bull calves (21 ± 2 days old) were fed milk replacer su...

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Autores principales:	Sena L. Field, Marcela G. Marrero, Lihe Liu, Francisco Peñagaricano, Jimena Laporta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7d0f459aea14e78b8bc979a922e6694
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spelling	oai:doaj.org-article:c7d0f459aea14e78b8bc979a922e66942021-12-02T15:38:23ZHistological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan10.1038/s41598-021-88443-w2045-2322https://doaj.org/article/c7d0f459aea14e78b8bc979a922e66942021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88443-whttps://doaj.org/toc/2045-2322Abstract In mammals, peripheral serotonin is involved in regulating energy balance. Herein, we characterized the transcriptomic profile and microstructure of adipose and muscle in pre-weaned calves with increased circulating serotonin. Holstein bull calves (21 ± 2 days old) were fed milk replacer supplemented with saline (CON, 8 mL/day n = 4) or 5-hydroxytryptophan (5-HTP, 90 mg/day, n = 4) for 10 consecutive days. Calves were euthanized on d10 to harvest adipose and muscle for RNA-Sequencing and histological analyses. Twenty-two genes were differentially expressed in adipose, and 33 in muscle. Notably, Interferon gamma inducible protein-47 was highly expressed and upregulated in muscle and adipose (avg. log FC = 6.5). Enriched pathways in adipose tissue revealed serotonin’s participation in lipid metabolism and PPAR signaling. In muscle, enriched pathways were related to histone acetyltransferase binding, Jak-STAT signaling, PI3K-Akt signaling and cell proliferation. Supplementation of 5-HTP increased cell proliferation and total cell number in adipose and muscle. Adipocyte surface area was smaller and muscle fiber area was not different in the 5-HTP group. Manipulating the serotonin pathway, through oral supplementation of 5-HTP, influences signaling pathways and cellular processes in adipose and muscle related to endocrine and metabolic functions which might translate into improvements in calf growth and development.Sena L. FieldMarcela G. MarreroLihe LiuFrancisco PeñagaricanoJimena LaportaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sena L. Field Marcela G. Marrero Lihe Liu Francisco Peñagaricano Jimena Laporta Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
description	Abstract In mammals, peripheral serotonin is involved in regulating energy balance. Herein, we characterized the transcriptomic profile and microstructure of adipose and muscle in pre-weaned calves with increased circulating serotonin. Holstein bull calves (21 ± 2 days old) were fed milk replacer supplemented with saline (CON, 8 mL/day n = 4) or 5-hydroxytryptophan (5-HTP, 90 mg/day, n = 4) for 10 consecutive days. Calves were euthanized on d10 to harvest adipose and muscle for RNA-Sequencing and histological analyses. Twenty-two genes were differentially expressed in adipose, and 33 in muscle. Notably, Interferon gamma inducible protein-47 was highly expressed and upregulated in muscle and adipose (avg. log FC = 6.5). Enriched pathways in adipose tissue revealed serotonin’s participation in lipid metabolism and PPAR signaling. In muscle, enriched pathways were related to histone acetyltransferase binding, Jak-STAT signaling, PI3K-Akt signaling and cell proliferation. Supplementation of 5-HTP increased cell proliferation and total cell number in adipose and muscle. Adipocyte surface area was smaller and muscle fiber area was not different in the 5-HTP group. Manipulating the serotonin pathway, through oral supplementation of 5-HTP, influences signaling pathways and cellular processes in adipose and muscle related to endocrine and metabolic functions which might translate into improvements in calf growth and development.
format	article
author	Sena L. Field Marcela G. Marrero Lihe Liu Francisco Peñagaricano Jimena Laporta
author_facet	Sena L. Field Marcela G. Marrero Lihe Liu Francisco Peñagaricano Jimena Laporta
author_sort	Sena L. Field
title	Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
title_short	Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
title_full	Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
title_fullStr	Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
title_full_unstemmed	Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
title_sort	histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c7d0f459aea14e78b8bc979a922e6694
work_keys_str_mv	AT senalfield histologicalandtranscriptomicanalysisofadiposeandmuscleofdairycalvessupplementedwith5hydroxytryptophan AT marcelagmarrero histologicalandtranscriptomicanalysisofadiposeandmuscleofdairycalvessupplementedwith5hydroxytryptophan AT liheliu histologicalandtranscriptomicanalysisofadiposeandmuscleofdairycalvessupplementedwith5hydroxytryptophan AT franciscopenagaricano histologicalandtranscriptomicanalysisofadiposeandmuscleofdairycalvessupplementedwith5hydroxytryptophan AT jimenalaporta histologicalandtranscriptomicanalysisofadiposeandmuscleofdairycalvessupplementedwith5hydroxytryptophan
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Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan

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