Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM

Xu Wu, Yashan Li, Baohua Man, Dexuan Li The Third People’s Hospital of Yunnan Province, Department of Clinical Laboratory, Kunming, 650200, People’s Republic of ChinaCorrespondence: Dexuan LiDepartment of Clinical Laboratory, The Third People’s Hospital of Yunnan Provin...

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Autores principales:	Wu X, Li Y, Man B, Li D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	c-peptide insulin microrna-375 ogtt type 2 diabetes mellitus Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/c7e1ed7761ae4cfc9b7ecf6b62dd20bf
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spelling	oai:doaj.org-article:c7e1ed7761ae4cfc9b7ecf6b62dd20bf2021-12-02T12:58:34ZAssessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM1178-7007https://doaj.org/article/c7e1ed7761ae4cfc9b7ecf6b62dd20bf2021-03-01T00:00:00Zhttps://www.dovepress.com/assessing-microrna-375-levels-in-type-2-diabetes-mellitus-t2dm-patient-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Xu Wu, Yashan Li, Baohua Man, Dexuan Li The Third People’s Hospital of Yunnan Province, Department of Clinical Laboratory, Kunming, 650200, People’s Republic of ChinaCorrespondence: Dexuan LiDepartment of Clinical Laboratory, The Third People’s Hospital of Yunnan Province, No. 292 Beijing Road, Kunming, 650200, People’s Republic of ChinaEmail 147347278@qq.comPurpose: The pancreatic islet specific microRNA-375 (miR-375) is overexpressed in type 2 diabetes mellitus (T2DM) patients suppressing the glucose-induced insulin secretion. Thus, miR-375 may serve as a biomarker for the early prediction of T2DM among high-risk individuals. We conducted this clinical study to assess the significance of miR-375 among type 2 diabetes mellitus (T2DM) patients and their first-degree relatives.Patients and Methods: We included 56 Han Chinese individuals (N: NGT = 21, T2DM = 10, FD-NGT =13 and FD-T2DM = 12) who received medical health check-ups from January 2018 to September 2018 at The Third Hospital of Yunnan Province, China. They were categorized as normal glucose tolerance (NGT), T2DM, first-degree relatives with normal glucose tolerance (FD-NGT) and first-degree relatives with T2DM (FD-T2DM). OGTT, C-peptide and Insulin tests were performed to confirm the diagnosis. The miR-375 levels were determined by Quantitative real-time RT-PCR (qRT-PCR).Results: The OGTT test showed a significant difference in T2DM and FD-T2DM groups compared with NGT and FD-NGT (p< 0.05). Similar results were observed during C-peptide and insulin tests. Interestingly, the 2-hour insulin test showed FD-NGT group having a significantly higher mean ± standard error of (64.240 ± 12.775) compared to NGT (28.836 ± 10.875). Assessment of miR-375 expression levels in 4 groups showed a significant up-regulation in T2DM and FD-T2DM compared with NGT and FD-NGT groups. A slight increase in miRNA expression was observed in FD-NGT compared with NGT group but was not statistically significant.Conclusion: The OGTT, C-peptide and insulin tests revealed a statistically significant difference in T2DM and FD-T2DM compared with NGT and FD-NGT groups. A significantly higher miR-375 expression was also observed in T2DM and FD-T2DM groups compared with NGT and FD-NGT and thus, miR-375 may serve as a stable biomarker for the early prediction of T2DM among high-risk individuals.Keywords: C-peptide, insulin, microRNA-375, OGTT, type 2 diabetes mellitusWu XLi YMan BLi DDove Medical Pressarticlec-peptideinsulinmicrorna-375ogtttype 2 diabetes mellitusSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 1445-1451 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	c-peptide insulin microrna-375 ogtt type 2 diabetes mellitus Specialties of internal medicine RC581-951
spellingShingle	c-peptide insulin microrna-375 ogtt type 2 diabetes mellitus Specialties of internal medicine RC581-951 Wu X Li Y Man B Li D Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM
description	Xu Wu, Yashan Li, Baohua Man, Dexuan Li The Third People’s Hospital of Yunnan Province, Department of Clinical Laboratory, Kunming, 650200, People’s Republic of ChinaCorrespondence: Dexuan LiDepartment of Clinical Laboratory, The Third People’s Hospital of Yunnan Province, No. 292 Beijing Road, Kunming, 650200, People’s Republic of ChinaEmail 147347278@qq.comPurpose: The pancreatic islet specific microRNA-375 (miR-375) is overexpressed in type 2 diabetes mellitus (T2DM) patients suppressing the glucose-induced insulin secretion. Thus, miR-375 may serve as a biomarker for the early prediction of T2DM among high-risk individuals. We conducted this clinical study to assess the significance of miR-375 among type 2 diabetes mellitus (T2DM) patients and their first-degree relatives.Patients and Methods: We included 56 Han Chinese individuals (N: NGT = 21, T2DM = 10, FD-NGT =13 and FD-T2DM = 12) who received medical health check-ups from January 2018 to September 2018 at The Third Hospital of Yunnan Province, China. They were categorized as normal glucose tolerance (NGT), T2DM, first-degree relatives with normal glucose tolerance (FD-NGT) and first-degree relatives with T2DM (FD-T2DM). OGTT, C-peptide and Insulin tests were performed to confirm the diagnosis. The miR-375 levels were determined by Quantitative real-time RT-PCR (qRT-PCR).Results: The OGTT test showed a significant difference in T2DM and FD-T2DM groups compared with NGT and FD-NGT (p< 0.05). Similar results were observed during C-peptide and insulin tests. Interestingly, the 2-hour insulin test showed FD-NGT group having a significantly higher mean ± standard error of (64.240 ± 12.775) compared to NGT (28.836 ± 10.875). Assessment of miR-375 expression levels in 4 groups showed a significant up-regulation in T2DM and FD-T2DM compared with NGT and FD-NGT groups. A slight increase in miRNA expression was observed in FD-NGT compared with NGT group but was not statistically significant.Conclusion: The OGTT, C-peptide and insulin tests revealed a statistically significant difference in T2DM and FD-T2DM compared with NGT and FD-NGT groups. A significantly higher miR-375 expression was also observed in T2DM and FD-T2DM groups compared with NGT and FD-NGT and thus, miR-375 may serve as a stable biomarker for the early prediction of T2DM among high-risk individuals.Keywords: C-peptide, insulin, microRNA-375, OGTT, type 2 diabetes mellitus
format	article
author	Wu X Li Y Man B Li D
author_facet	Wu X Li Y Man B Li D
author_sort	Wu X
title	Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM
title_short	Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM
title_full	Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM
title_fullStr	Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM
title_full_unstemmed	Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM
title_sort	assessing microrna-375 levels in type 2 diabetes mellitus (t2dm) patients and their first-degree relatives with t2dm
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/c7e1ed7761ae4cfc9b7ecf6b62dd20bf
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Assessing MicroRNA-375 Levels in Type 2 Diabetes Mellitus (T2DM) Patients and Their First-Degree Relatives with T2DM

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