Distinct dynamics of endocytic clathrin-coated pits and coated plaques.

Clathrin is the scaffold of a conserved molecular machinery that has evolved to capture membrane patches, which then pinch off to become traffic carriers. These carriers are the principal vehicles of receptor-mediated endocytosis and are the major route of traffic from plasma membrane to endosomes....

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Autores principales:	Saveez Saffarian, Emanuele Cocucci, Tomas Kirchhausen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c7e352c618f94120ab847597bebfef0d
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spelling	oai:doaj.org-article:c7e352c618f94120ab847597bebfef0d2021-11-25T05:34:02ZDistinct dynamics of endocytic clathrin-coated pits and coated plaques.1544-91731545-788510.1371/journal.pbio.1000191https://doaj.org/article/c7e352c618f94120ab847597bebfef0d2009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19809571/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Clathrin is the scaffold of a conserved molecular machinery that has evolved to capture membrane patches, which then pinch off to become traffic carriers. These carriers are the principal vehicles of receptor-mediated endocytosis and are the major route of traffic from plasma membrane to endosomes. We report here the use of in vivo imaging data, obtained from spinning disk confocal and total internal reflection fluorescence microscopy, to distinguish between two modes of endocytic clathrin coat formation, which we designate as "coated pits" and "coated plaques." Coated pits are small, rapidly forming structures that deform the underlying membrane by progressive recruitment of clathrin, adaptors, and other regulatory proteins. They ultimately close off and bud inward to form coated vesicles. Coated plaques are longer-lived structures with larger and less sharply curved coats; their clathrin lattices do not close off, but instead move inward from the cell surface shortly before membrane fission. Local remodeling of actin filaments is essential for the formation, inward movement, and dissolution of plaques, but it is not required for normal formation and budding of coated pits in the cells we have studied. We conclude that there are at least two distinct modes of clathrin coat formation at the plasma membrane--classical coated pits and coated plaques--and that these two assemblies interact quite differently with other intracellular structures.Saveez SaffarianEmanuele CocucciTomas KirchhausenPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 9, p e1000191 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Saveez Saffarian Emanuele Cocucci Tomas Kirchhausen Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
description	Clathrin is the scaffold of a conserved molecular machinery that has evolved to capture membrane patches, which then pinch off to become traffic carriers. These carriers are the principal vehicles of receptor-mediated endocytosis and are the major route of traffic from plasma membrane to endosomes. We report here the use of in vivo imaging data, obtained from spinning disk confocal and total internal reflection fluorescence microscopy, to distinguish between two modes of endocytic clathrin coat formation, which we designate as "coated pits" and "coated plaques." Coated pits are small, rapidly forming structures that deform the underlying membrane by progressive recruitment of clathrin, adaptors, and other regulatory proteins. They ultimately close off and bud inward to form coated vesicles. Coated plaques are longer-lived structures with larger and less sharply curved coats; their clathrin lattices do not close off, but instead move inward from the cell surface shortly before membrane fission. Local remodeling of actin filaments is essential for the formation, inward movement, and dissolution of plaques, but it is not required for normal formation and budding of coated pits in the cells we have studied. We conclude that there are at least two distinct modes of clathrin coat formation at the plasma membrane--classical coated pits and coated plaques--and that these two assemblies interact quite differently with other intracellular structures.
format	article
author	Saveez Saffarian Emanuele Cocucci Tomas Kirchhausen
author_facet	Saveez Saffarian Emanuele Cocucci Tomas Kirchhausen
author_sort	Saveez Saffarian
title	Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
title_short	Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
title_full	Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
title_fullStr	Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
title_full_unstemmed	Distinct dynamics of endocytic clathrin-coated pits and coated plaques.
title_sort	distinct dynamics of endocytic clathrin-coated pits and coated plaques.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/c7e352c618f94120ab847597bebfef0d
work_keys_str_mv	AT saveezsaffarian distinctdynamicsofendocyticclathrincoatedpitsandcoatedplaques AT emanuelecocucci distinctdynamicsofendocyticclathrincoatedpitsandcoatedplaques AT tomaskirchhausen distinctdynamicsofendocyticclathrincoatedpitsandcoatedplaques
_version_	1718414602353508352

Distinct dynamics of endocytic clathrin-coated pits and coated plaques.

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