Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription

Summary: Nuclear transfer systems represent the efficient means to reprogram a cell and in theory provide a basis for investigating the development of endangered species. However, conventional nuclear transfer using oocytes of laboratory animals does not allow reprogramming of cross-species nuclei o...

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Autores principales:	Junko Tomikawa, Christopher A. Penfold, Takuma Kamiya, Risa Hibino, Ayumi Kosaka, Masayuki Anzai, Kazuya Matsumoto, Kei Miyamoto
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell biology Stem cells research Developmental biology Science Q
Acceso en línea:	https://doaj.org/article/c7e3d6fbfc5345d496084183d44da4a4
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spelling	oai:doaj.org-article:c7e3d6fbfc5345d496084183d44da4a42021-11-20T05:09:29ZCell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription2589-004210.1016/j.isci.2021.103290https://doaj.org/article/c7e3d6fbfc5345d496084183d44da4a42021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012591https://doaj.org/toc/2589-0042Summary: Nuclear transfer systems represent the efficient means to reprogram a cell and in theory provide a basis for investigating the development of endangered species. However, conventional nuclear transfer using oocytes of laboratory animals does not allow reprogramming of cross-species nuclei owing to defects in cell divisions and activation of embryonic genes. Here, we show that somatic nuclei transferred into mouse four-cell embryos arrested at the G2/M phase undergo reprogramming toward the embryonic state. Remarkably, genome-wide transcriptional reprogramming is induced within a day, and ZFP281 is important for this replication-free reprogramming. This system further enables transcriptional reprogramming of cells from Oryx dammah, now extinct in the wild. Thus, our findings indicate that arrested mouse embryos are competent to induce intra- and cross-species reprogramming. The direct induction of embryonic transcripts from diverse genomes paves a unique approach for identifying mechanisms of transcriptional reprogramming and genome activation from a diverse range of species.Junko TomikawaChristopher A. PenfoldTakuma KamiyaRisa HibinoAyumi KosakaMasayuki AnzaiKazuya MatsumotoKei MiyamotoElsevierarticleCell biologyStem cells researchDevelopmental biologyScienceQENiScience, Vol 24, Iss 11, Pp 103290- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell biology Stem cells research Developmental biology Science Q
spellingShingle	Cell biology Stem cells research Developmental biology Science Q Junko Tomikawa Christopher A. Penfold Takuma Kamiya Risa Hibino Ayumi Kosaka Masayuki Anzai Kazuya Matsumoto Kei Miyamoto Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
description	Summary: Nuclear transfer systems represent the efficient means to reprogram a cell and in theory provide a basis for investigating the development of endangered species. However, conventional nuclear transfer using oocytes of laboratory animals does not allow reprogramming of cross-species nuclei owing to defects in cell divisions and activation of embryonic genes. Here, we show that somatic nuclei transferred into mouse four-cell embryos arrested at the G2/M phase undergo reprogramming toward the embryonic state. Remarkably, genome-wide transcriptional reprogramming is induced within a day, and ZFP281 is important for this replication-free reprogramming. This system further enables transcriptional reprogramming of cells from Oryx dammah, now extinct in the wild. Thus, our findings indicate that arrested mouse embryos are competent to induce intra- and cross-species reprogramming. The direct induction of embryonic transcripts from diverse genomes paves a unique approach for identifying mechanisms of transcriptional reprogramming and genome activation from a diverse range of species.
format	article
author	Junko Tomikawa Christopher A. Penfold Takuma Kamiya Risa Hibino Ayumi Kosaka Masayuki Anzai Kazuya Matsumoto Kei Miyamoto
author_facet	Junko Tomikawa Christopher A. Penfold Takuma Kamiya Risa Hibino Ayumi Kosaka Masayuki Anzai Kazuya Matsumoto Kei Miyamoto
author_sort	Junko Tomikawa
title	Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
title_short	Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
title_full	Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
title_fullStr	Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
title_full_unstemmed	Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
title_sort	cell division- and dna replication-free reprogramming of somatic nuclei for embryonic transcription
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c7e3d6fbfc5345d496084183d44da4a4
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Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription

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