Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus

Abstract The high degree of conservation of toll-like receptors (TLRs), and yet their subtle variations for better adaptation of species in the host–pathogen arms race make them worthy candidates for understanding evolution. We have attempted to track the trend of TLR evolution in the most diverse v...

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Autores principales: Manisha Priyam, Sanjay K. Gupta, Biplab Sarkar, T. R. Sharma, A. Pattanayak
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Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/c7e960af82b74e5588e2e7b849da16c8
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spelling oai:doaj.org-article:c7e960af82b74e5588e2e7b849da16c82021-12-02T16:08:54ZVariation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus10.1038/s41598-020-78347-62045-2322https://doaj.org/article/c7e960af82b74e5588e2e7b849da16c82020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78347-6https://doaj.org/toc/2045-2322Abstract The high degree of conservation of toll-like receptors (TLRs), and yet their subtle variations for better adaptation of species in the host–pathogen arms race make them worthy candidates for understanding evolution. We have attempted to track the trend of TLR evolution in the most diverse vertebrate group—teleosts, where Clarias batrachus was given emphasis, considering its traits for terrestrial adaptation. Eleven C. batrachus TLRs (TLR1, 2, 3, 5, 7, 8 9, 13, 22, 25, 26) were identified in this study which clustered in proximity to its Siluriformes relative orthologues in the phylogenetic analysis of 228 TLRs from 25 teleosts. Ten TLRs (TLR1, 2, 3, 5, 7, 8 9, 13, 21, 22) with at least 15 member orthologues for each alignment were processed for selection pressure and coevolutionary analysis. TLR1, 7, 8 and 9 were found to be under positive selection in the alignment-wide test. TLR1 also showed maximum episodic diversification in its clades while the teleost group Eupercaria showed the maximum divergence in their TLR repertoire. Episodic diversification was evident in C. batrachus TLR1 and 7 alignments. These results present a strong evidence of a divergent TLR repertoire in teleosts which may be contributing towards species-specific variation in TLR functions.Manisha PriyamSanjay K. GuptaBiplab SarkarT. R. SharmaA. PattanayakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Manisha Priyam
Sanjay K. Gupta
Biplab Sarkar
T. R. Sharma
A. Pattanayak
Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus
description Abstract The high degree of conservation of toll-like receptors (TLRs), and yet their subtle variations for better adaptation of species in the host–pathogen arms race make them worthy candidates for understanding evolution. We have attempted to track the trend of TLR evolution in the most diverse vertebrate group—teleosts, where Clarias batrachus was given emphasis, considering its traits for terrestrial adaptation. Eleven C. batrachus TLRs (TLR1, 2, 3, 5, 7, 8 9, 13, 22, 25, 26) were identified in this study which clustered in proximity to its Siluriformes relative orthologues in the phylogenetic analysis of 228 TLRs from 25 teleosts. Ten TLRs (TLR1, 2, 3, 5, 7, 8 9, 13, 21, 22) with at least 15 member orthologues for each alignment were processed for selection pressure and coevolutionary analysis. TLR1, 7, 8 and 9 were found to be under positive selection in the alignment-wide test. TLR1 also showed maximum episodic diversification in its clades while the teleost group Eupercaria showed the maximum divergence in their TLR repertoire. Episodic diversification was evident in C. batrachus TLR1 and 7 alignments. These results present a strong evidence of a divergent TLR repertoire in teleosts which may be contributing towards species-specific variation in TLR functions.
format article
author Manisha Priyam
Sanjay K. Gupta
Biplab Sarkar
T. R. Sharma
A. Pattanayak
author_facet Manisha Priyam
Sanjay K. Gupta
Biplab Sarkar
T. R. Sharma
A. Pattanayak
author_sort Manisha Priyam
title Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus
title_short Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus
title_full Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus
title_fullStr Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus
title_full_unstemmed Variation in selection constraints on teleost TLRs with emphasis on their repertoire in the Walking catfish, Clarias batrachus
title_sort variation in selection constraints on teleost tlrs with emphasis on their repertoire in the walking catfish, clarias batrachus
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/c7e960af82b74e5588e2e7b849da16c8
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