MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer

Abstract Background MicroRNAs (miRNAs) have been reported to play significant roles in non-small-cell lung cancer (NSCLC). However, the roles of microRNA (miR)-1915-3p in NSCLC remain unclear. In this study, we aimed to explore the biological functions of miR-1915-3p in NSCLC. Methods The expression...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hongli Pan, Zhenhua Pan, Fengjie Guo, Fanrong Meng, Lingling Zu, Yaguang Fan, Yang Li, Mengjie Li, Xinxin Du, Xiuwen Zhang, Yi Shao, Mingming Wei, Xuebing Li, Qinghua Zhou
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	NSCLC miR-1915-3p Invasion Migration SET Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/c7ef73183b9c4453afa82643468b845d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c7ef73183b9c4453afa82643468b845d
record_format	dspace
spelling	oai:doaj.org-article:c7ef73183b9c4453afa82643468b845d2021-11-14T12:29:58ZMicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer10.1186/s12885-021-08961-81471-2407https://doaj.org/article/c7ef73183b9c4453afa82643468b845d2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-08961-8https://doaj.org/toc/1471-2407Abstract Background MicroRNAs (miRNAs) have been reported to play significant roles in non-small-cell lung cancer (NSCLC). However, the roles of microRNA (miR)-1915-3p in NSCLC remain unclear. In this study, we aimed to explore the biological functions of miR-1915-3p in NSCLC. Methods The expression of miR-1915-3p and SET nuclear proto-oncogene (SET) in NSCLC tissues were examined by quantitative real-time PCR (qRT-PCR). Migratory and invasive abilities of lung cancer were tested by wound healing and transwell invasion assay. The direct target genes of miR-1915-3p were measured by dual-luciferase reporter assay and western blot. Finally, the regulation between METTL3/YTHDF2/KLF4 axis and miR-1915-3p were evaluated by qRT-PCR, promoter reporter assay and chromatin immunoprecipitation (CHIP). Results miR-1915-3p was downregulated in NSCLC tissues and cell lines, and inversely associated with clinical TNM stage and overall survival. Functional assays showed that miR-1915-3p significantly suppressed migration, invasion and epithelial-mesenchymal transition (EMT) in NSCLC cells. Furthermore, miR-1915-3p directly bound to the 3′untranslated region (3′UTR) of SET and modulated the expression of SET. SET inhibition could recapitulate the inhibitory effects on cell migration, invasion and EMT of miR-1915-3p, and restoration of SET expression could abrogate these effects induced by miR-1915-3p through JNK/Jun and NF-κB signaling pathways. What’s more, miR-1915-3p expression was regulated by METTL3/YTHDF2 m6A axis through transcription factor KLF4. Conclusions These findings demonstrate that miR-1915-3p function as a tumor suppressor by targeting SET and may have an anti-metastatic therapeutic potential for lung cancer treatment.Hongli PanZhenhua PanFengjie GuoFanrong MengLingling ZuYaguang FanYang LiMengjie LiXinxin DuXiuwen ZhangYi ShaoMingming WeiXuebing LiQinghua ZhouBMCarticleNSCLCmiR-1915-3pInvasionMigrationSETNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NSCLC miR-1915-3p Invasion Migration SET Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	NSCLC miR-1915-3p Invasion Migration SET Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Hongli Pan Zhenhua Pan Fengjie Guo Fanrong Meng Lingling Zu Yaguang Fan Yang Li Mengjie Li Xinxin Du Xiuwen Zhang Yi Shao Mingming Wei Xuebing Li Qinghua Zhou MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer
description	Abstract Background MicroRNAs (miRNAs) have been reported to play significant roles in non-small-cell lung cancer (NSCLC). However, the roles of microRNA (miR)-1915-3p in NSCLC remain unclear. In this study, we aimed to explore the biological functions of miR-1915-3p in NSCLC. Methods The expression of miR-1915-3p and SET nuclear proto-oncogene (SET) in NSCLC tissues were examined by quantitative real-time PCR (qRT-PCR). Migratory and invasive abilities of lung cancer were tested by wound healing and transwell invasion assay. The direct target genes of miR-1915-3p were measured by dual-luciferase reporter assay and western blot. Finally, the regulation between METTL3/YTHDF2/KLF4 axis and miR-1915-3p were evaluated by qRT-PCR, promoter reporter assay and chromatin immunoprecipitation (CHIP). Results miR-1915-3p was downregulated in NSCLC tissues and cell lines, and inversely associated with clinical TNM stage and overall survival. Functional assays showed that miR-1915-3p significantly suppressed migration, invasion and epithelial-mesenchymal transition (EMT) in NSCLC cells. Furthermore, miR-1915-3p directly bound to the 3′untranslated region (3′UTR) of SET and modulated the expression of SET. SET inhibition could recapitulate the inhibitory effects on cell migration, invasion and EMT of miR-1915-3p, and restoration of SET expression could abrogate these effects induced by miR-1915-3p through JNK/Jun and NF-κB signaling pathways. What’s more, miR-1915-3p expression was regulated by METTL3/YTHDF2 m6A axis through transcription factor KLF4. Conclusions These findings demonstrate that miR-1915-3p function as a tumor suppressor by targeting SET and may have an anti-metastatic therapeutic potential for lung cancer treatment.
format	article
author	Hongli Pan Zhenhua Pan Fengjie Guo Fanrong Meng Lingling Zu Yaguang Fan Yang Li Mengjie Li Xinxin Du Xiuwen Zhang Yi Shao Mingming Wei Xuebing Li Qinghua Zhou
author_facet	Hongli Pan Zhenhua Pan Fengjie Guo Fanrong Meng Lingling Zu Yaguang Fan Yang Li Mengjie Li Xinxin Du Xiuwen Zhang Yi Shao Mingming Wei Xuebing Li Qinghua Zhou
author_sort	Hongli Pan
title	MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer
title_short	MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer
title_full	MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer
title_fullStr	MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer
title_full_unstemmed	MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer
title_sort	microrna-1915-3p inhibits cell migration and invasion by targeting set in non-small-cell lung cancer
publisher	BMC
publishDate	2021
url	https://doaj.org/article/c7ef73183b9c4453afa82643468b845d
work_keys_str_mv	AT honglipan microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT zhenhuapan microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT fengjieguo microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT fanrongmeng microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT linglingzu microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT yaguangfan microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT yangli microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT mengjieli microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT xinxindu microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT xiuwenzhang microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT yishao microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT mingmingwei microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT xuebingli microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer AT qinghuazhou microrna19153pinhibitscellmigrationandinvasionbytargetingsetinnonsmallcelllungcancer
_version_	1718429193094561792

MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer

Ejemplares similares