Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model

Ruiqi Wu,* Guanying Gao,* Yan Xu Department of Sports Medicine, Peking University Third Hospital, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan Xu Tel +86 13701130401Email yanxu@139.comPurpose: Developmental dysplasia of the hip (DDH)...

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Autores principales:	Wu R, Gao G, Xu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	: electrospun fibers acetabulum reconstruction bone marrow stem cells osteogenic growth factor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c7f4254355e64f60b9fd3d45024ccddd
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spelling	oai:doaj.org-article:c7f4254355e64f60b9fd3d45024ccddd2021-12-02T15:22:39ZElectrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model1178-2013https://doaj.org/article/c7f4254355e64f60b9fd3d45024ccddd2020-09-01T00:00:00Zhttps://www.dovepress.com/electrospun-fibers-immobilized-with-bmp-2-mediated-by-polydopamine-com-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ruiqi Wu,* Guanying Gao,* Yan Xu Department of Sports Medicine, Peking University Third Hospital, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan Xu Tel +86 13701130401Email yanxu@139.comPurpose: Developmental dysplasia of the hip (DDH) can increase the pressure between the joints, which causes secondary hip osteoarthritis. The aim of the present study was to fabricate poly(D, L-lactic acid)-poly(ethylene glycol)-poly(D, L-lactic acid) (PELA) electrospun fibrous scaffolds, immobilized with bone morphogenetic protein-2 (BMP-2), to repair the acetabulum defects.Methods: The characteristics of PELA electrospun were analyzed using scanning electron microscopy, the release kinetics of BMP-2 in vitro were analyzed using enzyme-linked immunosorbent assays. Human mesenchymal stem cells (hMSCs) were used for in vitro experiments, the biocompatibility of the electrospinning materials was investigated using a cell counting kit-8 (CCK-8) kit, and osteogenic differentiation was tested via alkaline phosphatase (ALP) activity and relative gene expression. Eighteen miniature pig animal models were used in the in vivo experiment. The pigs were sacrificed at 24 weeks after surgery, and the reconstructed acetabulum was evaluated using histological sections.Results: Structural analysis revealed that the diameter of the PELA electrospun fiber was 0.8195 ± 0.16 μm. The PELA electrospun fiber materials showed good hydrophilicity and biocompatibility and could continuously release BMP-2 within 27 days: 16.07 ± 0.11 ng of BMP-2 was released from the scaffold. A total of sixteen implants fully filled the defects, and hematoxylin and eosin staining and Goldner’s trichrome staining showed that the simple tendon group (T group) was mostly fibrous tissues, lots of fibroblasts and small amounts of chondrocytes were observed in the polydopamine-coated electrospun fiber group (DP group). The DP plus BMP-2 (DPB) group showed a large number of chondrocytes and partial new bone tissues.Conclusion: PELA electrospun fibrous scaffolds are good sustained-release carriers, which can not only induce implant differentiation into cartilage and bone but also are completely degraded without toxicity. Therefore, the material can be used for bone and cartilage regeneration.Keywords: electrospun fibers, acetabulum reconstruction, bone marrow stem cells, osteogenic growth factorWu RGao GXu YDove Medical Pressarticle: electrospun fibersacetabulum reconstructionbone marrow stem cellsosteogenic growth factorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6563-6577 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	: electrospun fibers acetabulum reconstruction bone marrow stem cells osteogenic growth factor Medicine (General) R5-920
spellingShingle	: electrospun fibers acetabulum reconstruction bone marrow stem cells osteogenic growth factor Medicine (General) R5-920 Wu R Gao G Xu Y Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model
description	Ruiqi Wu,* Guanying Gao,* Yan Xu Department of Sports Medicine, Peking University Third Hospital, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan Xu Tel +86 13701130401Email yanxu@139.comPurpose: Developmental dysplasia of the hip (DDH) can increase the pressure between the joints, which causes secondary hip osteoarthritis. The aim of the present study was to fabricate poly(D, L-lactic acid)-poly(ethylene glycol)-poly(D, L-lactic acid) (PELA) electrospun fibrous scaffolds, immobilized with bone morphogenetic protein-2 (BMP-2), to repair the acetabulum defects.Methods: The characteristics of PELA electrospun were analyzed using scanning electron microscopy, the release kinetics of BMP-2 in vitro were analyzed using enzyme-linked immunosorbent assays. Human mesenchymal stem cells (hMSCs) were used for in vitro experiments, the biocompatibility of the electrospinning materials was investigated using a cell counting kit-8 (CCK-8) kit, and osteogenic differentiation was tested via alkaline phosphatase (ALP) activity and relative gene expression. Eighteen miniature pig animal models were used in the in vivo experiment. The pigs were sacrificed at 24 weeks after surgery, and the reconstructed acetabulum was evaluated using histological sections.Results: Structural analysis revealed that the diameter of the PELA electrospun fiber was 0.8195 ± 0.16 μm. The PELA electrospun fiber materials showed good hydrophilicity and biocompatibility and could continuously release BMP-2 within 27 days: 16.07 ± 0.11 ng of BMP-2 was released from the scaffold. A total of sixteen implants fully filled the defects, and hematoxylin and eosin staining and Goldner’s trichrome staining showed that the simple tendon group (T group) was mostly fibrous tissues, lots of fibroblasts and small amounts of chondrocytes were observed in the polydopamine-coated electrospun fiber group (DP group). The DP plus BMP-2 (DPB) group showed a large number of chondrocytes and partial new bone tissues.Conclusion: PELA electrospun fibrous scaffolds are good sustained-release carriers, which can not only induce implant differentiation into cartilage and bone but also are completely degraded without toxicity. Therefore, the material can be used for bone and cartilage regeneration.Keywords: electrospun fibers, acetabulum reconstruction, bone marrow stem cells, osteogenic growth factor
format	article
author	Wu R Gao G Xu Y
author_facet	Wu R Gao G Xu Y
author_sort	Wu R
title	Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model
title_short	Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model
title_full	Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model
title_fullStr	Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model
title_full_unstemmed	Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model
title_sort	electrospun fibers immobilized with bmp-2 mediated by polydopamine combined with autogenous tendon to repair developmental dysplasia of the hip in a porcine model
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/c7f4254355e64f60b9fd3d45024ccddd
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Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model

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