Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture

Abstract The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a natu...

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Autores principales:	Nymul Khan, Yukari Maezato, Ryan S. McClure, Colin J. Brislawn, Jennifer M. Mobberley, Nancy Isern, William B. Chrisler, Lye Meng Markillie, Brett M. Barney, Hyun-Seob Song, William C. Nelson, Hans C. Bernstein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7f655fb6f8f41f3a20d2783e62fb91e
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spelling	oai:doaj.org-article:c7f655fb6f8f41f3a20d2783e62fb91e2021-12-02T15:08:49ZPhenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture10.1038/s41598-017-18630-12045-2322https://doaj.org/article/c7f655fb6f8f41f3a20d2783e62fb91e2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18630-1https://doaj.org/toc/2045-2322Abstract The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended on nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.Nymul KhanYukari MaezatoRyan S. McClureColin J. BrislawnJennifer M. MobberleyNancy IsernWilliam B. ChrislerLye Meng MarkillieBrett M. BarneyHyun-Seob SongWilliam C. NelsonHans C. BernsteinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nymul Khan Yukari Maezato Ryan S. McClure Colin J. Brislawn Jennifer M. Mobberley Nancy Isern William B. Chrisler Lye Meng Markillie Brett M. Barney Hyun-Seob Song William C. Nelson Hans C. Bernstein Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
description	Abstract The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended on nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.
format	article
author	Nymul Khan Yukari Maezato Ryan S. McClure Colin J. Brislawn Jennifer M. Mobberley Nancy Isern William B. Chrisler Lye Meng Markillie Brett M. Barney Hyun-Seob Song William C. Nelson Hans C. Bernstein
author_facet	Nymul Khan Yukari Maezato Ryan S. McClure Colin J. Brislawn Jennifer M. Mobberley Nancy Isern William B. Chrisler Lye Meng Markillie Brett M. Barney Hyun-Seob Song William C. Nelson Hans C. Bernstein
author_sort	Nymul Khan
title	Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
title_short	Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
title_full	Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
title_fullStr	Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
title_full_unstemmed	Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
title_sort	phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c7f655fb6f8f41f3a20d2783e62fb91e
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Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture

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