A general strategy for expanding polymerase function by droplet microfluidics

Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase.

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Autores principales:	Andrew C. Larsen, Matthew R. Dunn, Andrew Hatch, Sujay P. Sau, Cody Youngbull, John C. Chaput
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c7f8937b5925459eaf6e4d4fd43af4f8
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spelling	oai:doaj.org-article:c7f8937b5925459eaf6e4d4fd43af4f82021-12-02T14:39:54ZA general strategy for expanding polymerase function by droplet microfluidics10.1038/ncomms112352041-1723https://doaj.org/article/c7f8937b5925459eaf6e4d4fd43af4f82016-04-01T00:00:00Zhttps://doi.org/10.1038/ncomms11235https://doaj.org/toc/2041-1723Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase.Andrew C. LarsenMatthew R. DunnAndrew HatchSujay P. SauCody YoungbullJohn C. ChaputNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Andrew C. Larsen Matthew R. Dunn Andrew Hatch Sujay P. Sau Cody Youngbull John C. Chaput A general strategy for expanding polymerase function by droplet microfluidics
description	Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase.
format	article
author	Andrew C. Larsen Matthew R. Dunn Andrew Hatch Sujay P. Sau Cody Youngbull John C. Chaput
author_facet	Andrew C. Larsen Matthew R. Dunn Andrew Hatch Sujay P. Sau Cody Youngbull John C. Chaput
author_sort	Andrew C. Larsen
title	A general strategy for expanding polymerase function by droplet microfluidics
title_short	A general strategy for expanding polymerase function by droplet microfluidics
title_full	A general strategy for expanding polymerase function by droplet microfluidics
title_fullStr	A general strategy for expanding polymerase function by droplet microfluidics
title_full_unstemmed	A general strategy for expanding polymerase function by droplet microfluidics
title_sort	general strategy for expanding polymerase function by droplet microfluidics
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/c7f8937b5925459eaf6e4d4fd43af4f8
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A general strategy for expanding polymerase function by droplet microfluidics

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