Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Luiz Cláudio da Silva,1 Thiago Garcia,1 Michela Mori,2 Giuseppina Sandri,2 Maria Cristina Bonferoni,2 Priscila V Finotelli,1 Leonardo P Cinelli,1 Carla Caramella,2 Lúcio M Cabral11Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil; 2Department of...

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Autores principales: da Silva LC, Garcia T, Mori M, Sandri G, Bonferoni MC, Finotelli PV, Cinelli LP, Caramella C, Cabral LM
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/c7fe7df807084d3dbf32b48b7e03ecca
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spelling oai:doaj.org-article:c7fe7df807084d3dbf32b48b7e03ecca2021-12-02T02:14:38ZPreparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity1176-91141178-2013https://doaj.org/article/c7fe7df807084d3dbf32b48b7e03ecca2012-06-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-polysaccharide-based-nanoparticles-a10138https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Luiz Cláudio da Silva,1 Thiago Garcia,1 Michela Mori,2 Giuseppina Sandri,2 Maria Cristina Bonferoni,2 Priscila V Finotelli,1 Leonardo P Cinelli,1 Carla Caramella,2 Lúcio M Cabral11Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil; 2Department of Drug Sciences, University of Pavia, Pavia, ItalyAbstract: The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.Keywords: nanoparticles, chitosan, chondroitin sulfate, fucoidan, aPTT, Caco-2da Silva LCGarcia TMori MSandri GBonferoni MCFinotelli PVCinelli LPCaramella CCabral LMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2975-2986 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
da Silva LC
Garcia T
Mori M
Sandri G
Bonferoni MC
Finotelli PV
Cinelli LP
Caramella C
Cabral LM
Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
description Luiz Cláudio da Silva,1 Thiago Garcia,1 Michela Mori,2 Giuseppina Sandri,2 Maria Cristina Bonferoni,2 Priscila V Finotelli,1 Leonardo P Cinelli,1 Carla Caramella,2 Lúcio M Cabral11Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil; 2Department of Drug Sciences, University of Pavia, Pavia, ItalyAbstract: The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.Keywords: nanoparticles, chitosan, chondroitin sulfate, fucoidan, aPTT, Caco-2
format article
author da Silva LC
Garcia T
Mori M
Sandri G
Bonferoni MC
Finotelli PV
Cinelli LP
Caramella C
Cabral LM
author_facet da Silva LC
Garcia T
Mori M
Sandri G
Bonferoni MC
Finotelli PV
Cinelli LP
Caramella C
Cabral LM
author_sort da Silva LC
title Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
title_short Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
title_full Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
title_fullStr Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
title_full_unstemmed Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
title_sort preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/c7fe7df807084d3dbf32b48b7e03ecca
work_keys_str_mv AT dasilvalc preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT garciat preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT morim preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT sandrig preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT bonferonimc preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT finotellipv preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT cinellilp preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT caramellac preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
AT cabrallm preparationandcharacterizationofpolysaccharidebasednanoparticleswithanticoagulantactivity
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