The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study

Purpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices a...

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Autores principales:	Suganthi Mohanarangam, Dhayanand John Victor, Sangeetha Subramanian, P SG Prakash
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	absorbable collagen sponge nanocrystalline hydroxyapatite collagen osteoblast cells periostin Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/c8049955e0e94c98abe7ba6affd99dfe
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spelling	oai:doaj.org-article:c8049955e0e94c98abe7ba6affd99dfe2021-11-12T10:23:42ZThe influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study0972-124X10.4103/jisp.jisp_396_20https://doaj.org/article/c8049955e0e94c98abe7ba6affd99dfe2021-01-01T00:00:00Zhttp://www.jisponline.com/article.asp?issn=0972-124X;year=2021;volume=25;issue=6;spage=480;epage=484;aulast=Mohanarangamhttps://doaj.org/toc/0972-124XPurpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices as follows: Group A: absorbable collagen sponge (ACS), Group B: ACS impregnated with recombinant human periostin, Group C: nanocrystalline hydroxyapatite collagen (NcHC), and Group D: NcHC impregnated with recombinanant human periostin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to evaluate cell viability as well as adhesion and proliferation on 2nd, 5th, and 7th day. Osteoblast activity was studied using alkaline phosphatase (ALP) assay for the study groups. Results: The periostin-treated absorbable collagen matrices showed a statistically significant increase in the osteoblast adhesion compared to periostin-treated NcHC on days 2, 5, and 7 (P < 0.001). The osteoblast activity as evaluated by ALP assay showed that there is increased activity in the periostin-treated ACS compared to the periostin-treated NcHC. Conclusion: From the observations of this study, it is evident that Periostin has a significant role in the modulating cellular response of the osteoblast cells. Further, incorporation of periostin into the ACS has been shown to increase the cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells.Suganthi MohanarangamDhayanand John VictorSangeetha SubramanianP SG PrakashWolters Kluwer Medknow Publicationsarticleabsorbable collagen spongenanocrystalline hydroxyapatite collagenosteoblast cellsperiostinDentistryRK1-715ENJournal of Indian Society of Periodontology, Vol 25, Iss 6, Pp 480-484 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	absorbable collagen sponge nanocrystalline hydroxyapatite collagen osteoblast cells periostin Dentistry RK1-715
spellingShingle	absorbable collagen sponge nanocrystalline hydroxyapatite collagen osteoblast cells periostin Dentistry RK1-715 Suganthi Mohanarangam Dhayanand John Victor Sangeetha Subramanian P SG Prakash The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
description	Purpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices as follows: Group A: absorbable collagen sponge (ACS), Group B: ACS impregnated with recombinant human periostin, Group C: nanocrystalline hydroxyapatite collagen (NcHC), and Group D: NcHC impregnated with recombinanant human periostin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to evaluate cell viability as well as adhesion and proliferation on 2nd, 5th, and 7th day. Osteoblast activity was studied using alkaline phosphatase (ALP) assay for the study groups. Results: The periostin-treated absorbable collagen matrices showed a statistically significant increase in the osteoblast adhesion compared to periostin-treated NcHC on days 2, 5, and 7 (P < 0.001). The osteoblast activity as evaluated by ALP assay showed that there is increased activity in the periostin-treated ACS compared to the periostin-treated NcHC. Conclusion: From the observations of this study, it is evident that Periostin has a significant role in the modulating cellular response of the osteoblast cells. Further, incorporation of periostin into the ACS has been shown to increase the cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells.
format	article
author	Suganthi Mohanarangam Dhayanand John Victor Sangeetha Subramanian P SG Prakash
author_facet	Suganthi Mohanarangam Dhayanand John Victor Sangeetha Subramanian P SG Prakash
author_sort	Suganthi Mohanarangam
title	The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
title_short	The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
title_full	The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
title_fullStr	The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
title_full_unstemmed	The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study
title_sort	influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - an in vitro study
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/c8049955e0e94c98abe7ba6affd99dfe
work_keys_str_mv	AT suganthimohanarangam theinfluenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT dhayanandjohnvictor theinfluenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT sangeethasubramanian theinfluenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT psgprakash theinfluenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT suganthimohanarangam influenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT dhayanandjohnvictor influenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT sangeethasubramanian influenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy AT psgprakash influenceofperiostinonosteoblasticadhesionandproliferationoncollagenmatricesaninvitrostudy
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The influence of periostin on osteoblastic adhesion and proliferation on collagen matrices - An in vitro study

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