New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer

Abstract Pteris vittata is an arsenic (As) hyperaccumulator plant that accumulates a large amount of As into fronds and rhizomes (around 16,000 mg/kg in both after 16 weeks hydroponic cultivation with 30 mg/L arsenate). However, the sequence of long-distance transport of As in this hyperaccumulator...

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Autores principales:	Yi Huang-Takeshi Kohda, Zhaojie Qian, Mei-Fang Chien, Keisuke Miyauchi, Ginro Endo, Nobuo Suzui, Yong-Gen Yin, Naoki Kawachi, Hayato Ikeda, Hiroshi Watabe, Hidetoshi Kikunaga, Nobuyuki Kitajima, Chihiro Inoue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c815f25cadea48328987f4cf8f55be66
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spelling	oai:doaj.org-article:c815f25cadea48328987f4cf8f55be662021-12-02T15:39:59ZNew evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer10.1038/s41598-021-91374-12045-2322https://doaj.org/article/c815f25cadea48328987f4cf8f55be662021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91374-1https://doaj.org/toc/2045-2322Abstract Pteris vittata is an arsenic (As) hyperaccumulator plant that accumulates a large amount of As into fronds and rhizomes (around 16,000 mg/kg in both after 16 weeks hydroponic cultivation with 30 mg/L arsenate). However, the sequence of long-distance transport of As in this hyperaccumulator plant is unclear. In this study, we used a positron-emitting tracer imaging system (PETIS) for the first time to obtain noninvasive serial images of As behavior in living plants with positron-emitting 74As-labeled tracer. We found that As kept accumulating in rhizomes as in fronds of P. vittata, whereas As was retained in roots of a non-accumulator plant Arabidopsis thaliana. Autoradiograph results of As distribution in P. vittata showed that with low As exposure, As was predominantly accumulated in young fronds and the midrib and rachis of mature fronds. Under high As exposure, As accumulation shifted from young fronds to mature fronds, especially in the margin of pinna, which resulted in necrotic symptoms, turning the marginal color to gray and then brown. Our results indicated that the function of rhizomes in P. vittata was As accumulation and the regulation of As translocation to the mature fronds to protect the young fronds under high As exposure.Yi Huang-Takeshi KohdaZhaojie QianMei-Fang ChienKeisuke MiyauchiGinro EndoNobuo SuzuiYong-Gen YinNaoki KawachiHayato IkedaHiroshi WatabeHidetoshi KikunagaNobuyuki KitajimaChihiro InoueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Huang-Takeshi Kohda Zhaojie Qian Mei-Fang Chien Keisuke Miyauchi Ginro Endo Nobuo Suzui Yong-Gen Yin Naoki Kawachi Hayato Ikeda Hiroshi Watabe Hidetoshi Kikunaga Nobuyuki Kitajima Chihiro Inoue New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer
description	Abstract Pteris vittata is an arsenic (As) hyperaccumulator plant that accumulates a large amount of As into fronds and rhizomes (around 16,000 mg/kg in both after 16 weeks hydroponic cultivation with 30 mg/L arsenate). However, the sequence of long-distance transport of As in this hyperaccumulator plant is unclear. In this study, we used a positron-emitting tracer imaging system (PETIS) for the first time to obtain noninvasive serial images of As behavior in living plants with positron-emitting 74As-labeled tracer. We found that As kept accumulating in rhizomes as in fronds of P. vittata, whereas As was retained in roots of a non-accumulator plant Arabidopsis thaliana. Autoradiograph results of As distribution in P. vittata showed that with low As exposure, As was predominantly accumulated in young fronds and the midrib and rachis of mature fronds. Under high As exposure, As accumulation shifted from young fronds to mature fronds, especially in the margin of pinna, which resulted in necrotic symptoms, turning the marginal color to gray and then brown. Our results indicated that the function of rhizomes in P. vittata was As accumulation and the regulation of As translocation to the mature fronds to protect the young fronds under high As exposure.
format	article
author	Yi Huang-Takeshi Kohda Zhaojie Qian Mei-Fang Chien Keisuke Miyauchi Ginro Endo Nobuo Suzui Yong-Gen Yin Naoki Kawachi Hayato Ikeda Hiroshi Watabe Hidetoshi Kikunaga Nobuyuki Kitajima Chihiro Inoue
author_facet	Yi Huang-Takeshi Kohda Zhaojie Qian Mei-Fang Chien Keisuke Miyauchi Ginro Endo Nobuo Suzui Yong-Gen Yin Naoki Kawachi Hayato Ikeda Hiroshi Watabe Hidetoshi Kikunaga Nobuyuki Kitajima Chihiro Inoue
author_sort	Yi Huang-Takeshi Kohda
title	New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer
title_short	New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer
title_full	New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer
title_fullStr	New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer
title_full_unstemmed	New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer
title_sort	new evidence of arsenic translocation and accumulation in pteris vittata from real-time imaging using positron-emitting 74as tracer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c815f25cadea48328987f4cf8f55be66
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New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer

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