CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.

Guardado en:

Detalles Bibliográficos
Autores principales:	Daisuke Miki, Wenxin Zhang, Wenjie Zeng, Zhengyan Feng, Jian-Kang Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c81bd1b528a94970a1a314b129cdc17f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c81bd1b528a94970a1a314b129cdc17f
record_format	dspace
spelling	oai:doaj.org-article:c81bd1b528a94970a1a314b129cdc17f2021-12-02T16:56:53ZCRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation10.1038/s41467-018-04416-02041-1723https://doaj.org/article/c81bd1b528a94970a1a314b129cdc17f2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04416-0https://doaj.org/toc/2041-1723Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.Daisuke MikiWenxin ZhangWenjie ZengZhengyan FengJian-Kang ZhuNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Daisuke Miki Wenxin Zhang Wenjie Zeng Zhengyan Feng Jian-Kang Zhu CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
description	Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.
format	article
author	Daisuke Miki Wenxin Zhang Wenjie Zeng Zhengyan Feng Jian-Kang Zhu
author_facet	Daisuke Miki Wenxin Zhang Wenjie Zeng Zhengyan Feng Jian-Kang Zhu
author_sort	Daisuke Miki
title	CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_short	CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_full	CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_fullStr	CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_full_unstemmed	CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_sort	crispr/cas9-mediated gene targeting in arabidopsis using sequential transformation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c81bd1b528a94970a1a314b129cdc17f
work_keys_str_mv	AT daisukemiki crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation AT wenxinzhang crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation AT wenjiezeng crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation AT zhengyanfeng crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation AT jiankangzhu crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation
_version_	1718382706185732096

CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

Ejemplares similares