CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.

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Detalles Bibliográficos
Autores principales: Daisuke Miki, Wenxin Zhang, Wenjie Zeng, Zhengyan Feng, Jian-Kang Zhu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
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Acceso en línea:https://doaj.org/article/c81bd1b528a94970a1a314b129cdc17f
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