CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.
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Main Authors: | Daisuke Miki, Wenxin Zhang, Wenjie Zeng, Zhengyan Feng, Jian-Kang Zhu |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2018
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Subjects: | |
Online Access: | https://doaj.org/article/c81bd1b528a94970a1a314b129cdc17f |
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