Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure

Abstract Heart failure (HF) is the main cause of mortality worldwide, particularly in the elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) is the gold standard biomarker for HF diagnosis and therapy monitoring. It is determined in blood samples by the immunochemical methods generally ad...

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Autores principales:	Francesca G. Bellagambi, Christina Petersen, Pietro Salvo, Silvia Ghimenti, Maria Franzini, Denise Biagini, Marie Hangouët, Maria Giovanna Trivella, Fabio Di Francesco, Aldo Paolicchi, Abdelhamid Errachid, Roger Fuoco, Tommaso Lomonaco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c820dc6e104a4b5ca20ea5c6e8245319
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spelling	oai:doaj.org-article:c820dc6e104a4b5ca20ea5c6e82453192021-12-02T17:44:54ZDetermination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure10.1038/s41598-021-92488-22045-2322https://doaj.org/article/c820dc6e104a4b5ca20ea5c6e82453192021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92488-2https://doaj.org/toc/2045-2322Abstract Heart failure (HF) is the main cause of mortality worldwide, particularly in the elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) is the gold standard biomarker for HF diagnosis and therapy monitoring. It is determined in blood samples by the immunochemical methods generally adopted by most laboratories. Saliva analysis is a powerful tool for clinical applications, mainly due to its non-invasive and less risky sampling. This study describes a validated analytical procedure for NT-proBNP determination in saliva samples using a commercial Enzyme-Linked Immuno-Sorbent Assay. Linearity, matrix effect, sensitivity, recovery and assay-precision were evaluated. The analytical approach showed a linear behaviour of the signal throughout the concentrations tested, with a minimum detectable dose of 1 pg/mL, a satisfactory NT-proBNP recovery (95–110%), and acceptable precision (coefficient of variation ≤ 10%). Short-term (3 weeks) and long-term (5 months) stability of NT-proBNP in saliva samples under the storage conditions most frequently used in clinical laboratories (4, − 20, and − 80 °C) was also investigated and showed that the optimal storage conditions were at − 20 °C for up to 2.5 months. Finally, the method was tested for the determination of NT-proBNP in saliva samples collected from ten hospitalized acute HF patients. Preliminary results indicate a decrease in NT-proBNP in saliva from admission to discharge, thus suggesting that this procedure is an effective saliva-based point-of-care device for HF monitoring.Francesca G. BellagambiChristina PetersenPietro SalvoSilvia GhimentiMaria FranziniDenise BiaginiMarie HangouëtMaria Giovanna TrivellaFabio Di FrancescoAldo PaolicchiAbdelhamid ErrachidRoger FuocoTommaso LomonacoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesca G. Bellagambi Christina Petersen Pietro Salvo Silvia Ghimenti Maria Franzini Denise Biagini Marie Hangouët Maria Giovanna Trivella Fabio Di Francesco Aldo Paolicchi Abdelhamid Errachid Roger Fuoco Tommaso Lomonaco Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
description	Abstract Heart failure (HF) is the main cause of mortality worldwide, particularly in the elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) is the gold standard biomarker for HF diagnosis and therapy monitoring. It is determined in blood samples by the immunochemical methods generally adopted by most laboratories. Saliva analysis is a powerful tool for clinical applications, mainly due to its non-invasive and less risky sampling. This study describes a validated analytical procedure for NT-proBNP determination in saliva samples using a commercial Enzyme-Linked Immuno-Sorbent Assay. Linearity, matrix effect, sensitivity, recovery and assay-precision were evaluated. The analytical approach showed a linear behaviour of the signal throughout the concentrations tested, with a minimum detectable dose of 1 pg/mL, a satisfactory NT-proBNP recovery (95–110%), and acceptable precision (coefficient of variation ≤ 10%). Short-term (3 weeks) and long-term (5 months) stability of NT-proBNP in saliva samples under the storage conditions most frequently used in clinical laboratories (4, − 20, and − 80 °C) was also investigated and showed that the optimal storage conditions were at − 20 °C for up to 2.5 months. Finally, the method was tested for the determination of NT-proBNP in saliva samples collected from ten hospitalized acute HF patients. Preliminary results indicate a decrease in NT-proBNP in saliva from admission to discharge, thus suggesting that this procedure is an effective saliva-based point-of-care device for HF monitoring.
format	article
author	Francesca G. Bellagambi Christina Petersen Pietro Salvo Silvia Ghimenti Maria Franzini Denise Biagini Marie Hangouët Maria Giovanna Trivella Fabio Di Francesco Aldo Paolicchi Abdelhamid Errachid Roger Fuoco Tommaso Lomonaco
author_facet	Francesca G. Bellagambi Christina Petersen Pietro Salvo Silvia Ghimenti Maria Franzini Denise Biagini Marie Hangouët Maria Giovanna Trivella Fabio Di Francesco Aldo Paolicchi Abdelhamid Errachid Roger Fuoco Tommaso Lomonaco
author_sort	Francesca G. Bellagambi
title	Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
title_short	Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
title_full	Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
title_fullStr	Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
title_full_unstemmed	Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
title_sort	determination and stability of n-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c820dc6e104a4b5ca20ea5c6e8245319
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Determination and stability of N-terminal pro-brain natriuretic peptide in saliva samples for monitoring heart failure

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