A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines

Abstract The human placenta plays a crucial role as the interface between mother and fetus. It represents a unique tissue that undergoes morphological as well as functional changes on the cellular and tissue level throughout pregnancy. To better understand how the placenta works, a variety of techni...

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Autores principales:	Mario Rothbauer, Nilaykumar Patel, Hajnalka Gondola, Monika Siwetz, Berthold Huppertz, Peter Ertl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c835f30028104d538b551ecc446a9c90
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spelling	oai:doaj.org-article:c835f30028104d538b551ecc446a9c902021-12-02T15:06:14ZA comparative study of five physiological key parameters between four different human trophoblast-derived cell lines10.1038/s41598-017-06364-z2045-2322https://doaj.org/article/c835f30028104d538b551ecc446a9c902017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06364-zhttps://doaj.org/toc/2045-2322Abstract The human placenta plays a crucial role as the interface between mother and fetus. It represents a unique tissue that undergoes morphological as well as functional changes on the cellular and tissue level throughout pregnancy. To better understand how the placenta works, a variety of techniques has been developed to re-create this complex physiological barrier in vitro. However, due to the low availability of freshly isolated primary cells, choriocarcinoma cell lines remain the usual suspects as in vitro models for placental research. Here, we present a comparative study on the functional aspects of the choriocarcinoma cell lines BeWo, JAR and Jeg-3, as well as the first trimester trophoblast cell line ACH-3P as placental in vitro barrier models for endocrine and transport studies. Functional assays including tight junction immunostaining, sodium fluorescein retardation, trans epithelial resistance, glucose transport, hormone secretion as well as size-dependent polystyrene nanoparticle transport were performed using the four cell types to evaluate key functional parameters of each cell line to act a relevant in vitro placental barrier model.Mario RothbauerNilaykumar PatelHajnalka GondolaMonika SiwetzBerthold HuppertzPeter ErtlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mario Rothbauer Nilaykumar Patel Hajnalka Gondola Monika Siwetz Berthold Huppertz Peter Ertl A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
description	Abstract The human placenta plays a crucial role as the interface between mother and fetus. It represents a unique tissue that undergoes morphological as well as functional changes on the cellular and tissue level throughout pregnancy. To better understand how the placenta works, a variety of techniques has been developed to re-create this complex physiological barrier in vitro. However, due to the low availability of freshly isolated primary cells, choriocarcinoma cell lines remain the usual suspects as in vitro models for placental research. Here, we present a comparative study on the functional aspects of the choriocarcinoma cell lines BeWo, JAR and Jeg-3, as well as the first trimester trophoblast cell line ACH-3P as placental in vitro barrier models for endocrine and transport studies. Functional assays including tight junction immunostaining, sodium fluorescein retardation, trans epithelial resistance, glucose transport, hormone secretion as well as size-dependent polystyrene nanoparticle transport were performed using the four cell types to evaluate key functional parameters of each cell line to act a relevant in vitro placental barrier model.
format	article
author	Mario Rothbauer Nilaykumar Patel Hajnalka Gondola Monika Siwetz Berthold Huppertz Peter Ertl
author_facet	Mario Rothbauer Nilaykumar Patel Hajnalka Gondola Monika Siwetz Berthold Huppertz Peter Ertl
author_sort	Mario Rothbauer
title	A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
title_short	A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
title_full	A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
title_fullStr	A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
title_full_unstemmed	A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
title_sort	comparative study of five physiological key parameters between four different human trophoblast-derived cell lines
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c835f30028104d538b551ecc446a9c90
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A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines

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