Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells

Karolina Karnas,1,2 Tomasz Str&aogon;czek,3 Czes&lstrok;aw Kapusta,3 Ma&lstrok;gorzata Lekka,4 Joanna Duli&nacute;ska-Litewka,2 Anna Karewicz1 1Department of Chemistry, Jagiellonian University, Kraków, Poland; 2Chair of Medical Biochemistry, Jagiellonian University Medical College, K...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karnas K, Strączek T, Kapusta C, Lekka M, Dulińska-Litewka J, Karewicz A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	superparamagnetic iron oxide nanoparticles n-cadherin antibody circulating tumor cells cancer magnetic cell capture Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c84d487de9da419ba9c3af9f226fb84b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c84d487de9da419ba9c3af9f226fb84b
record_format	dspace
spelling	oai:doaj.org-article:c84d487de9da419ba9c3af9f226fb84b2021-12-02T18:53:07ZSpecific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells1178-2013https://doaj.org/article/c84d487de9da419ba9c3af9f226fb84b2021-09-01T00:00:00Zhttps://www.dovepress.com/specific-binding-of-novel-spion-based-system-bearing-anti-n-cadherin-a-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Karolina Karnas,1,2 Tomasz Str&aogon;czek,3 Czes&lstrok;aw Kapusta,3 Ma&lstrok;gorzata Lekka,4 Joanna Duli&nacute;ska-Litewka,2 Anna Karewicz1 1Department of Chemistry, Jagiellonian University, Kraków, Poland; 2Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków, Poland; 3AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Department of Solid State Physics, Kraków, Poland; 4Department of Biophysical Microstructures, Institute of Nuclear Physics, Polish Academy of Sciences, Kraków, PolandCorrespondence: Anna KarewiczDepartment of Chemistry, Jagiellonian University, Gronostajowa 2, Kraków, 30-387, PolandTel +48 12 686 25 33Fax +48 12 686 27 50Email karewicz@chemia.uj.edu.plJoanna Duli&nacute;ska-LitewkaChair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, Kraków, 31-034, PolandTel +48 12 422 74 00Fax +48 12 422 32 72Email joanna.dulinska-litewka@uj.edu.plPurpose: Epithelial–mesenchymal (EMT) transition plays an important role in metastasis and is accompanied by an upregulation of N-cadherin expression. A new nanoparticulate system (SPION/CCh/N-cad) based on superparamagnetic iron oxide nanoparticles, stabilized with a cationic derivative of chitosan and surface-modified with anti-N-cadherin antibody, was synthetized for the effective capture of N-cadherin expressing circulating tumor cells (CTC).Methods: The morphology, physicochemical, and magnetic properties of the system were evaluated using dynamic light scattering (DLS), fluorescence spectroscopy, Mössbauer spectroscopy, magnetometry, and fluorescence spectroscopy. Atomic force microscopy (AFM), confocal microscopy and flow cytometry were used to study the interaction of our nanoparticulate system with N-cadherin expressed in prostate cancer cell lines (PC-3 and DU 145). A purpose-built cuvette was used in the cancer cell capture experiments.Results: The obtained nanoparticles were a spherical, stable colloid, and exhibited excellent magnetic properties. Biological experiments confirmed that the novel SPION/CCh/N-cad system interacts specifically with N-cadherin present on the cell surface. Preliminary studies on the magnetic capture of PC-3 cells using the obtained nanoparticles were successful. Incubation times as short as 1 minute were sufficient for the synthesized system to effectively bind to the PC-3 cells.Conclusion: Results obtained for our system suggest a possibility of using it to capture CTC in the flow conditions.Keywords: superparamagnetic iron oxide nanoparticles, N-cadherin, antibody, circulating tumor cells, cancer, magnetic cell captureKarnas KStrączek TKapusta CLekka MDulińska-Litewka JKarewicz ADove Medical Pressarticlesuperparamagnetic iron oxide nanoparticlesn-cadherinantibodycirculating tumor cellscancermagnetic cell captureMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 6537-6552 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	superparamagnetic iron oxide nanoparticles n-cadherin antibody circulating tumor cells cancer magnetic cell capture Medicine (General) R5-920
spellingShingle	superparamagnetic iron oxide nanoparticles n-cadherin antibody circulating tumor cells cancer magnetic cell capture Medicine (General) R5-920 Karnas K Strączek T Kapusta C Lekka M Dulińska-Litewka J Karewicz A Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
description	Karolina Karnas,1,2 Tomasz Str&aogon;czek,3 Czes&lstrok;aw Kapusta,3 Ma&lstrok;gorzata Lekka,4 Joanna Duli&nacute;ska-Litewka,2 Anna Karewicz1 1Department of Chemistry, Jagiellonian University, Kraków, Poland; 2Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków, Poland; 3AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Department of Solid State Physics, Kraków, Poland; 4Department of Biophysical Microstructures, Institute of Nuclear Physics, Polish Academy of Sciences, Kraków, PolandCorrespondence: Anna KarewiczDepartment of Chemistry, Jagiellonian University, Gronostajowa 2, Kraków, 30-387, PolandTel +48 12 686 25 33Fax +48 12 686 27 50Email karewicz@chemia.uj.edu.plJoanna Duli&nacute;ska-LitewkaChair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, Kraków, 31-034, PolandTel +48 12 422 74 00Fax +48 12 422 32 72Email joanna.dulinska-litewka@uj.edu.plPurpose: Epithelial–mesenchymal (EMT) transition plays an important role in metastasis and is accompanied by an upregulation of N-cadherin expression. A new nanoparticulate system (SPION/CCh/N-cad) based on superparamagnetic iron oxide nanoparticles, stabilized with a cationic derivative of chitosan and surface-modified with anti-N-cadherin antibody, was synthetized for the effective capture of N-cadherin expressing circulating tumor cells (CTC).Methods: The morphology, physicochemical, and magnetic properties of the system were evaluated using dynamic light scattering (DLS), fluorescence spectroscopy, Mössbauer spectroscopy, magnetometry, and fluorescence spectroscopy. Atomic force microscopy (AFM), confocal microscopy and flow cytometry were used to study the interaction of our nanoparticulate system with N-cadherin expressed in prostate cancer cell lines (PC-3 and DU 145). A purpose-built cuvette was used in the cancer cell capture experiments.Results: The obtained nanoparticles were a spherical, stable colloid, and exhibited excellent magnetic properties. Biological experiments confirmed that the novel SPION/CCh/N-cad system interacts specifically with N-cadherin present on the cell surface. Preliminary studies on the magnetic capture of PC-3 cells using the obtained nanoparticles were successful. Incubation times as short as 1 minute were sufficient for the synthesized system to effectively bind to the PC-3 cells.Conclusion: Results obtained for our system suggest a possibility of using it to capture CTC in the flow conditions.Keywords: superparamagnetic iron oxide nanoparticles, N-cadherin, antibody, circulating tumor cells, cancer, magnetic cell capture
format	article
author	Karnas K Strączek T Kapusta C Lekka M Dulińska-Litewka J Karewicz A
author_facet	Karnas K Strączek T Kapusta C Lekka M Dulińska-Litewka J Karewicz A
author_sort	Karnas K
title	Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
title_short	Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
title_full	Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
title_fullStr	Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
title_full_unstemmed	Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells
title_sort	specific binding of novel spion-based system bearing anti-n-cadherin antibodies to prostate tumor cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/c84d487de9da419ba9c3af9f226fb84b
work_keys_str_mv	AT karnask specificbindingofnovelspionbasedsystembearingantincadherinantibodiestoprostatetumorcells AT straczekt specificbindingofnovelspionbasedsystembearingantincadherinantibodiestoprostatetumorcells AT kapustac specificbindingofnovelspionbasedsystembearingantincadherinantibodiestoprostatetumorcells AT lekkam specificbindingofnovelspionbasedsystembearingantincadherinantibodiestoprostatetumorcells AT dulinskalitewkaj specificbindingofnovelspionbasedsystembearingantincadherinantibodiestoprostatetumorcells AT karewicza specificbindingofnovelspionbasedsystembearingantincadherinantibodiestoprostatetumorcells
_version_	1718377352291942400

Specific Binding of Novel SPION-Based System Bearing Anti-N-Cadherin Antibodies to Prostate Tumor Cells

Ejemplares similares