An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils

Sheena Hunt,1 Mayumi Yoshida,2 Catherine EJ Davis,2 Nicholas S Greenhill,2 Paul F Davis21Promisia Integrative Ltd, Wellington, New Zealand; 2Trinity Bioactives Ltd, Wellington, New ZealandPurpose: To investigate the ability of a commercial extract from the medicinal plant Artemisia annua to modulat...

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Autores principales:	Hunt S, Yoshida M, Davis CEJ, Greenhill NS, Davis PF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c85298d9224c4296a1e8444e5bdd0d9b
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spelling	oai:doaj.org-article:c85298d9224c4296a1e8444e5bdd0d9b2021-12-02T07:15:37ZAn extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils1178-7031https://doaj.org/article/c85298d9224c4296a1e8444e5bdd0d9b2015-01-01T00:00:00Zhttp://www.dovepress.com/an-extract-of-the-medicinal-plant-artemisia-annua-modulates-production-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031 Sheena Hunt,1 Mayumi Yoshida,2 Catherine EJ Davis,2 Nicholas S Greenhill,2 Paul F Davis21Promisia Integrative Ltd, Wellington, New Zealand; 2Trinity Bioactives Ltd, Wellington, New ZealandPurpose: To investigate the ability of a commercial extract from the medicinal plant Artemisia annua to modulate production of the cytokine, tumor necrosis factor-alpha (TNF-α), and the cyclooxygenase (COX) inflammatory marker, prostaglandin E2 (PGE2) in activated neutrophils. Methods: Neutrophils were harvested from rat whole blood and cultured in the presence of plant extract or control samples. Neutrophils, except unactivated control cells, were activated with 10 µg/mL lipopolysaccharide (LPS). The cells were cultured with a range of different concentrations of the A. annua extracts (400–1 µg/mL) and artemisinin (200 and 100 µg/mL) and the supernatants were then tested by enzyme-linked immunosorbent assay (ELISA) for the concentrations of TNF-α and PGE2. Each sample was assayed in triplicate. Positive controls with an inhibitor were assayed in triplicate: chloroquine 2.58 and 5.16 µg/mL for TNF-α, and ibuprofen 400 µg/mL for PGE2. An unsupplemented group was also assessed in triplicate as a baseline control.Results: Neutrophils were stimulated to an inflammatory state by the addition of LPS. A. annua extract significantly inhibited TNF-α production by activated neutrophils in a dose-dependent manner. There was complete inhibition by the A. annua extract at 200, 100, and 50 µg/mL (all P≤0.0003). At A. annua extract concentrations of 25, 10, and 5 µg/mL, TNF-α production was inhibited by 89% (P<0.0001), 54% (P=0.0002), and 38% (P=0.0014), respectively. A. annua 1 µg/mL did not significantly inhibit TNF-α production (8.8%; P>0.05). Concentrations of 400, 200, and 100 µg/mL A. annua extract significantly inhibited PGE2 production by 87% (P=0.0128), 91% (P=0.0017), and 93% (P=0.0114), respectively.Conclusion: An extract of A. annua was shown to be a potent inhibitor of TNF-α and a strong inhibitor of PGE2 production in activated neutrophils at the concentrations tested. Further studies are warranted with this promising plant extract.Keywords: in vitro, TNF-α, COX-2, PGE2, artemisinin, ArthremHunt SYoshida MDavis CEJGreenhill NSDavis PFDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2015, Iss default, Pp 9-14 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Hunt S Yoshida M Davis CEJ Greenhill NS Davis PF An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils
description	Sheena Hunt,1 Mayumi Yoshida,2 Catherine EJ Davis,2 Nicholas S Greenhill,2 Paul F Davis21Promisia Integrative Ltd, Wellington, New Zealand; 2Trinity Bioactives Ltd, Wellington, New ZealandPurpose: To investigate the ability of a commercial extract from the medicinal plant Artemisia annua to modulate production of the cytokine, tumor necrosis factor-alpha (TNF-α), and the cyclooxygenase (COX) inflammatory marker, prostaglandin E2 (PGE2) in activated neutrophils. Methods: Neutrophils were harvested from rat whole blood and cultured in the presence of plant extract or control samples. Neutrophils, except unactivated control cells, were activated with 10 µg/mL lipopolysaccharide (LPS). The cells were cultured with a range of different concentrations of the A. annua extracts (400–1 µg/mL) and artemisinin (200 and 100 µg/mL) and the supernatants were then tested by enzyme-linked immunosorbent assay (ELISA) for the concentrations of TNF-α and PGE2. Each sample was assayed in triplicate. Positive controls with an inhibitor were assayed in triplicate: chloroquine 2.58 and 5.16 µg/mL for TNF-α, and ibuprofen 400 µg/mL for PGE2. An unsupplemented group was also assessed in triplicate as a baseline control.Results: Neutrophils were stimulated to an inflammatory state by the addition of LPS. A. annua extract significantly inhibited TNF-α production by activated neutrophils in a dose-dependent manner. There was complete inhibition by the A. annua extract at 200, 100, and 50 µg/mL (all P≤0.0003). At A. annua extract concentrations of 25, 10, and 5 µg/mL, TNF-α production was inhibited by 89% (P<0.0001), 54% (P=0.0002), and 38% (P=0.0014), respectively. A. annua 1 µg/mL did not significantly inhibit TNF-α production (8.8%; P>0.05). Concentrations of 400, 200, and 100 µg/mL A. annua extract significantly inhibited PGE2 production by 87% (P=0.0128), 91% (P=0.0017), and 93% (P=0.0114), respectively.Conclusion: An extract of A. annua was shown to be a potent inhibitor of TNF-α and a strong inhibitor of PGE2 production in activated neutrophils at the concentrations tested. Further studies are warranted with this promising plant extract.Keywords: in vitro, TNF-α, COX-2, PGE2, artemisinin, Arthrem
format	article
author	Hunt S Yoshida M Davis CEJ Greenhill NS Davis PF
author_facet	Hunt S Yoshida M Davis CEJ Greenhill NS Davis PF
author_sort	Hunt S
title	An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils
title_short	An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils
title_full	An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils
title_fullStr	An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils
title_full_unstemmed	An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils
title_sort	extract of the medicinal plant artemisia annua modulates production of inflammatory markers in activated neutrophils
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/c85298d9224c4296a1e8444e5bdd0d9b
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An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils

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