Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma
Abstract The prognosis of bladder urothelial carcinoma (BLCA) varies greatly even for patients with similar pathological characteristics. We conducted transcriptome sequencing on ten pairs of BLCA samples and adjacent normal tissues to identify differentially expressed genes. Anillin (ANLN) was iden...
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Nature Portfolio
2017
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oai:doaj.org-article:c8578fc724fb45c9a1d593735c9ec7b12021-12-02T16:08:23ZTranscriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma10.1038/s41598-017-02990-92045-2322https://doaj.org/article/c8578fc724fb45c9a1d593735c9ec7b12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02990-9https://doaj.org/toc/2045-2322Abstract The prognosis of bladder urothelial carcinoma (BLCA) varies greatly even for patients with similar pathological characteristics. We conducted transcriptome sequencing on ten pairs of BLCA samples and adjacent normal tissues to identify differentially expressed genes. Anillin (ANLN) was identified as a transcript that was significantly up-regulated in BLCA samples compared with normal tissues. Prognostic power of candidate gene was studied using qRT-PCR and immunohistochemistry on 40 and 209 patients, respectively. Patients with elevated ANLN expression level was correlated with poorer cancer-specific (median, 22.4 vs. 37.3 months, p = 0.001), progression-free (median, 19.7 vs. 27.9 months, p = 0.001) and recurrence-free survival (median, 17.1 vs. 25.2 months, p = 0.011) compared with low ANLN expression. Public datasets TCGA and NCBI-GEO were analyzed for external validation. Knockdown of ANLN in J82 and 5637 cells using small interfering RNA significantly inhibited cell proliferation, migration, and invasion ability. Moreover, knockdown of ANLN resulted in G2/M phase arrest and decreased expression of cyclin B1 and D1. Microarray analysis suggested that ANLN played a major role in cell migration and was closely associated with several cancer-related signaling pathways. In conclusion, ANLN was identified as a promising prognostic biomarker which could be used to stratify different risks of BLCA.Shuxiong ZengXiaowen YuChong MaRuixiang SongZhensheng ZhangXiaoyuan ZiXin ChenYang WangYongwei YuJunjie ZhaoRongchao WeiYinghao SunChuanliang XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Shuxiong Zeng Xiaowen Yu Chong Ma Ruixiang Song Zhensheng Zhang Xiaoyuan Zi Xin Chen Yang Wang Yongwei Yu Junjie Zhao Rongchao Wei Yinghao Sun Chuanliang Xu Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma |
| description |
Abstract The prognosis of bladder urothelial carcinoma (BLCA) varies greatly even for patients with similar pathological characteristics. We conducted transcriptome sequencing on ten pairs of BLCA samples and adjacent normal tissues to identify differentially expressed genes. Anillin (ANLN) was identified as a transcript that was significantly up-regulated in BLCA samples compared with normal tissues. Prognostic power of candidate gene was studied using qRT-PCR and immunohistochemistry on 40 and 209 patients, respectively. Patients with elevated ANLN expression level was correlated with poorer cancer-specific (median, 22.4 vs. 37.3 months, p = 0.001), progression-free (median, 19.7 vs. 27.9 months, p = 0.001) and recurrence-free survival (median, 17.1 vs. 25.2 months, p = 0.011) compared with low ANLN expression. Public datasets TCGA and NCBI-GEO were analyzed for external validation. Knockdown of ANLN in J82 and 5637 cells using small interfering RNA significantly inhibited cell proliferation, migration, and invasion ability. Moreover, knockdown of ANLN resulted in G2/M phase arrest and decreased expression of cyclin B1 and D1. Microarray analysis suggested that ANLN played a major role in cell migration and was closely associated with several cancer-related signaling pathways. In conclusion, ANLN was identified as a promising prognostic biomarker which could be used to stratify different risks of BLCA. |
| format |
article |
| author |
Shuxiong Zeng Xiaowen Yu Chong Ma Ruixiang Song Zhensheng Zhang Xiaoyuan Zi Xin Chen Yang Wang Yongwei Yu Junjie Zhao Rongchao Wei Yinghao Sun Chuanliang Xu |
| author_facet |
Shuxiong Zeng Xiaowen Yu Chong Ma Ruixiang Song Zhensheng Zhang Xiaoyuan Zi Xin Chen Yang Wang Yongwei Yu Junjie Zhao Rongchao Wei Yinghao Sun Chuanliang Xu |
| author_sort |
Shuxiong Zeng |
| title |
Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma |
| title_short |
Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma |
| title_full |
Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma |
| title_fullStr |
Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma |
| title_full_unstemmed |
Transcriptome sequencing identifies ANLN as a promising prognostic biomarker in bladder urothelial carcinoma |
| title_sort |
transcriptome sequencing identifies anln as a promising prognostic biomarker in bladder urothelial carcinoma |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/c8578fc724fb45c9a1d593735c9ec7b1 |
| work_keys_str_mv |
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