Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum

Background Golden2-Like (GLK) transcription factors are a type of transcriptional regulator in plants. They play a pivotal role in the plant physiological activity process and abiotic stress response. Methods In this study, the potential function of GLK family genes in Gossypium hirsutum was studied...

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Autores principales:	Zilin Zhao, Jiaran Shuang, Zhaoguo Li, Huimin Xiao, Yuling Liu, Tao Wang, Yangyang Wei, Shoulin Hu, Sumei Wan, Renhai Peng
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Gossypium hirsutum Golden2-like Gene family Phylogenetic analysis Abiotic stress Medicine R
Acceso en línea:	https://doaj.org/article/c85cf3731c604d87bf30a73a05d27928
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spelling	oai:doaj.org-article:c85cf3731c604d87bf30a73a05d279282021-11-18T15:05:37ZIdentification of the Golden-2-like transcription factors gene family in Gossypium hirsutum10.7717/peerj.124842167-8359https://doaj.org/article/c85cf3731c604d87bf30a73a05d279282021-11-01T00:00:00Zhttps://peerj.com/articles/12484.pdfhttps://peerj.com/articles/12484/https://doaj.org/toc/2167-8359Background Golden2-Like (GLK) transcription factors are a type of transcriptional regulator in plants. They play a pivotal role in the plant physiological activity process and abiotic stress response. Methods In this study, the potential function of GLK family genes in Gossypium hirsutum was studied based on genomic identification, phylogenetic analysis, chromosome mapping and cis-regulatory elements prediction. Gene expression of nine key genes were analyzed by qRT-PCR experiments. Results Herein, we identified a total of 146 GhGLK genes in Gossypium hirsutum, which were unevenly distributed on each of the chromosomes. There were significant differences in the number and location of genes between the At sub-genome and the Dt sub-genome. According to the phylogenetic analysis, they were divided into ten subgroups, each of which had very similar number and structure of exons and introns. Some cis-regulatory elements were identified through promoter analysis, including five types of elements related to abiotic stress response, five types of elements related to phytohormone and five types of elements involved in growth and development. Based on public transcriptome data analysis, we identified nine key GhGLKs involved in salt, cold, and drought stress. The qRT-PCR results showed that these genes had different expression patterns under these stress conditions, suggesting that GhGLK genes played an important role in abiotic stress response. This study laid a theoretical foundation for the screening and functional verification of genes related to stress resistance of GLK gene family in cotton.Zilin ZhaoJiaran ShuangZhaoguo LiHuimin XiaoYuling LiuTao WangYangyang WeiShoulin HuSumei WanRenhai PengPeerJ Inc.articleGossypium hirsutumGolden2-likeGene familyPhylogenetic analysisAbiotic stressMedicineRENPeerJ, Vol 9, p e12484 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gossypium hirsutum Golden2-like Gene family Phylogenetic analysis Abiotic stress Medicine R
spellingShingle	Gossypium hirsutum Golden2-like Gene family Phylogenetic analysis Abiotic stress Medicine R Zilin Zhao Jiaran Shuang Zhaoguo Li Huimin Xiao Yuling Liu Tao Wang Yangyang Wei Shoulin Hu Sumei Wan Renhai Peng Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum
description	Background Golden2-Like (GLK) transcription factors are a type of transcriptional regulator in plants. They play a pivotal role in the plant physiological activity process and abiotic stress response. Methods In this study, the potential function of GLK family genes in Gossypium hirsutum was studied based on genomic identification, phylogenetic analysis, chromosome mapping and cis-regulatory elements prediction. Gene expression of nine key genes were analyzed by qRT-PCR experiments. Results Herein, we identified a total of 146 GhGLK genes in Gossypium hirsutum, which were unevenly distributed on each of the chromosomes. There were significant differences in the number and location of genes between the At sub-genome and the Dt sub-genome. According to the phylogenetic analysis, they were divided into ten subgroups, each of which had very similar number and structure of exons and introns. Some cis-regulatory elements were identified through promoter analysis, including five types of elements related to abiotic stress response, five types of elements related to phytohormone and five types of elements involved in growth and development. Based on public transcriptome data analysis, we identified nine key GhGLKs involved in salt, cold, and drought stress. The qRT-PCR results showed that these genes had different expression patterns under these stress conditions, suggesting that GhGLK genes played an important role in abiotic stress response. This study laid a theoretical foundation for the screening and functional verification of genes related to stress resistance of GLK gene family in cotton.
format	article
author	Zilin Zhao Jiaran Shuang Zhaoguo Li Huimin Xiao Yuling Liu Tao Wang Yangyang Wei Shoulin Hu Sumei Wan Renhai Peng
author_facet	Zilin Zhao Jiaran Shuang Zhaoguo Li Huimin Xiao Yuling Liu Tao Wang Yangyang Wei Shoulin Hu Sumei Wan Renhai Peng
author_sort	Zilin Zhao
title	Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum
title_short	Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum
title_full	Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum
title_fullStr	Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum
title_full_unstemmed	Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum
title_sort	identification of the golden-2-like transcription factors gene family in gossypium hirsutum
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/c85cf3731c604d87bf30a73a05d27928
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Identification of the Golden-2-like transcription factors gene family in Gossypium hirsutum

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