Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells

Abstract Following access into the cell, colloidal silver nanoparticles exhibit generalized cytotoxic properties, thus appear as omnipotent microbicidal, but not suitable for systemic use unless are free of toxic effects on host cells. The AgNP-Serum-18 when prepared from silver nitrate, using dextr...

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Autores principales:	Rehana Parveen, Prasanta Kumar Maiti, Nabendu Murmu, Alokmay Datta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c86102c474cc405b866c8842bd64c7d6
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spelling	oai:doaj.org-article:c86102c474cc405b866c8842bd64c7d62021-12-02T15:57:21ZPreparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells10.1038/s41598-021-91031-72045-2322https://doaj.org/article/c86102c474cc405b866c8842bd64c7d62021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91031-7https://doaj.org/toc/2045-2322Abstract Following access into the cell, colloidal silver nanoparticles exhibit generalized cytotoxic properties, thus appear as omnipotent microbicidal, but not suitable for systemic use unless are free of toxic effects on host cells. The AgNP-Serum-18 when prepared from silver nitrate, using dextrose as reducing and group-matched homologous serum as a stabilizing agent, selective endocytosis, and oxidative stress-dependent bio-functional damages to the host are mostly eliminated. For their bio-mimicking outer coat, there is the least possibility of internalization into host cells or liberation of excess oxidants in circulation following interaction with erythrocytes or vascular endothelial cells. The presence of infection-specific antibodies in the serum can make such nano-conjugates more selective. A potent antimicrobial action and a wide margin of safety for mammalian cells in comparison with very similar PVA-capped silver nanoparticles have been demonstrated by the in-vitro challenge of such nanoparticles on different microbes, human liver cell-line, and in-vivo study on mice model. This may open up wide-range therapeutic prospects of colloidal nanoparticles.Rehana ParveenPrasanta Kumar MaitiNabendu MurmuAlokmay DattaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rehana Parveen Prasanta Kumar Maiti Nabendu Murmu Alokmay Datta Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
description	Abstract Following access into the cell, colloidal silver nanoparticles exhibit generalized cytotoxic properties, thus appear as omnipotent microbicidal, but not suitable for systemic use unless are free of toxic effects on host cells. The AgNP-Serum-18 when prepared from silver nitrate, using dextrose as reducing and group-matched homologous serum as a stabilizing agent, selective endocytosis, and oxidative stress-dependent bio-functional damages to the host are mostly eliminated. For their bio-mimicking outer coat, there is the least possibility of internalization into host cells or liberation of excess oxidants in circulation following interaction with erythrocytes or vascular endothelial cells. The presence of infection-specific antibodies in the serum can make such nano-conjugates more selective. A potent antimicrobial action and a wide margin of safety for mammalian cells in comparison with very similar PVA-capped silver nanoparticles have been demonstrated by the in-vitro challenge of such nanoparticles on different microbes, human liver cell-line, and in-vivo study on mice model. This may open up wide-range therapeutic prospects of colloidal nanoparticles.
format	article
author	Rehana Parveen Prasanta Kumar Maiti Nabendu Murmu Alokmay Datta
author_facet	Rehana Parveen Prasanta Kumar Maiti Nabendu Murmu Alokmay Datta
author_sort	Rehana Parveen
title	Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
title_short	Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
title_full	Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
title_fullStr	Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
title_full_unstemmed	Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
title_sort	preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c86102c474cc405b866c8842bd64c7d6
work_keys_str_mv	AT rehanaparveen preparationofserumcappedsilvernanoparticlesforselectivekillingofmicrobialcellssparinghostcells AT prasantakumarmaiti preparationofserumcappedsilvernanoparticlesforselectivekillingofmicrobialcellssparinghostcells AT nabendumurmu preparationofserumcappedsilvernanoparticlesforselectivekillingofmicrobialcellssparinghostcells AT alokmaydatta preparationofserumcappedsilvernanoparticlesforselectivekillingofmicrobialcellssparinghostcells
_version_	1718385356191039488

Preparation of serum capped silver nanoparticles for selective killing of microbial cells sparing host cells

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