Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media

Abstract The optical absorption coefficient of culture media is critical for the survival analysis of pathogens under optical irradiation. The quality of the results obtained from experiments relies on the optical analysis of the spatial distribution of fluence which also depends on the geometry of...

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Autores principales:	Juan Carlos Martínez-Antón, Alejandro Brun, Daniel Vázquez, Sandra Moreno, Antonio A. Fernández-Balbuena, Javier Alda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c8615bfe6bc046fda2f1e76a515af035
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spelling	oai:doaj.org-article:c8615bfe6bc046fda2f1e76a515af0352021-12-02T16:24:52ZDetermination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media10.1038/s41598-021-94648-w2045-2322https://doaj.org/article/c8615bfe6bc046fda2f1e76a515af0352021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94648-whttps://doaj.org/toc/2045-2322Abstract The optical absorption coefficient of culture media is critical for the survival analysis of pathogens under optical irradiation. The quality of the results obtained from experiments relies on the optical analysis of the spatial distribution of fluence which also depends on the geometry of the sample. In this contribution, we consider both the geometrical shape and the culture medium’s absorption coefficient to evaluate how the spatial distribution of optical radiation affects pathogens/viruses. In this work, we exposed SARS-CoV-2 to UV-C radiation ( $$\lambda$$ λ = 254 nm) and we calculated—considering the influence of the optical absorption of the culture medium—a characteristic inactivation fluence of $$F_i$$ F i = 4.7 J/m2, or an equivalent 10% survival (D90 dose) of 10.8 J/m2. Experimentally, we diluted the virus into sessile drops of Dulbecco’s Modified Eagle Medium to evaluate pathogen activity after controlled doses of UV irradiation. To validate the optical absorption mode, we carried out an additional experiment where we varied droplet size. Our model—including optical absorption and geometrical considerations—provides robust results among a variety of experimental situations, and represents our experimental conditions more accurately. These results will help to evaluate the capability of UV disinfecting strategies applied to a variety of everyday situations, including the case of micro-droplets generated by respiratory functions.Juan Carlos Martínez-AntónAlejandro BrunDaniel VázquezSandra MorenoAntonio A. Fernández-BalbuenaJavier AldaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Carlos Martínez-Antón Alejandro Brun Daniel Vázquez Sandra Moreno Antonio A. Fernández-Balbuena Javier Alda Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media
description	Abstract The optical absorption coefficient of culture media is critical for the survival analysis of pathogens under optical irradiation. The quality of the results obtained from experiments relies on the optical analysis of the spatial distribution of fluence which also depends on the geometry of the sample. In this contribution, we consider both the geometrical shape and the culture medium’s absorption coefficient to evaluate how the spatial distribution of optical radiation affects pathogens/viruses. In this work, we exposed SARS-CoV-2 to UV-C radiation ( $$\lambda$$ λ = 254 nm) and we calculated—considering the influence of the optical absorption of the culture medium—a characteristic inactivation fluence of $$F_i$$ F i = 4.7 J/m2, or an equivalent 10% survival (D90 dose) of 10.8 J/m2. Experimentally, we diluted the virus into sessile drops of Dulbecco’s Modified Eagle Medium to evaluate pathogen activity after controlled doses of UV irradiation. To validate the optical absorption mode, we carried out an additional experiment where we varied droplet size. Our model—including optical absorption and geometrical considerations—provides robust results among a variety of experimental situations, and represents our experimental conditions more accurately. These results will help to evaluate the capability of UV disinfecting strategies applied to a variety of everyday situations, including the case of micro-droplets generated by respiratory functions.
format	article
author	Juan Carlos Martínez-Antón Alejandro Brun Daniel Vázquez Sandra Moreno Antonio A. Fernández-Balbuena Javier Alda
author_facet	Juan Carlos Martínez-Antón Alejandro Brun Daniel Vázquez Sandra Moreno Antonio A. Fernández-Balbuena Javier Alda
author_sort	Juan Carlos Martínez-Antón
title	Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media
title_short	Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media
title_full	Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media
title_fullStr	Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media
title_full_unstemmed	Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media
title_sort	determination of the characteristic inactivation fluence for sars-cov-2 under uv-c radiation considering light absorption in culture media
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c8615bfe6bc046fda2f1e76a515af035
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Determination of the characteristic inactivation fluence for SARS-CoV-2 under UV-C radiation considering light absorption in culture media

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