Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats

Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats

Osama AA Ahmed,1,2 Usama A Fahmy,1 Rana Bakhaidar,1 Mohamed A El-Moselhy,3,4 Mohamed A Alfaleh,5 Al-Shaimaa F Ahmed,4 Asmaa SA Hammad,4 Hibah Aldawsari,1 Nabil A Alhakamy1,6–8 1Advanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia...

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Autores principales: Ahmed OAA, Fahmy UA, Bakhaidar R, El-Moselhy MA, Alfaleh MA, Ahmed ASF, Hammad ASA, Aldawsari H, Alhakamy NA
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2020
Materias:
natural products
gastric ulcer
pumpkin seed oil
nano-lipid carriers
optimization
box-behnken experimental design
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/c86166e567b4474dbe5eed68355a7906
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spelling oai:doaj.org-article:c86166e567b4474dbe5eed68355a79062021-12-02T09:53:56ZPumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats1178-2013https://doaj.org/article/c86166e567b4474dbe5eed68355a79062020-04-01T00:00:00Zhttps://www.dovepress.com/pumpkin-oilndashbased-nanostructured-lipid-carrier-system-for-antiulce-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Osama AA Ahmed,1,2 Usama A Fahmy,1 Rana Bakhaidar,1 Mohamed A El-Moselhy,3,4 Mohamed A Alfaleh,5 Al-Shaimaa F Ahmed,4 Asmaa SA Hammad,4 Hibah Aldawsari,1 Nabil A Alhakamy1,6–8 1Advanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 3Department of Pharmacology, School of Pharmacy, Ibn Sina National College, Jeddah 22413, Saudi Arabia; 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 5Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 6Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 7Center of Excellence for Drug Research and Pharmaceutical Industries, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 8King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCorrespondence: Osama AA AhmedAdvanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaTel +966 599120686Email oaahmed@kau.edu.saBackground: Peptic ulcer disease, a painful lesion of the gastric mucosa, is considered one of the most common gastrointestinal disorders. This study aims to investigate the formulation of pumpkin seed oil (PSO)-based nanostructured lipid carriers (NLCs) to utilize PSO as the liquid lipid component of NLCs and to achieve oil dispersion in the nano-range in the stomach.Methods: Box–Behnken design was utilized to deduce the optimum formula with minimum particle size. The optimized PSO-NLCs formula was investigated for gastric ulcer protective effects in Wistar rats by evaluating ulcer index and determination of gastric mucosa oxidative stress parameters.Results: PSO was successfully incorporated as the liquid lipid (LL) component of NLCs. The prepared optimum PSO-NLCs formula showed a size of 64.3 nm. Pretreatment of animals using the optimized PSO-NLCs formula showed significantly (p< 0.001) lower ulcer index compared to indomethacin alone group and significantly (p< 0.05) less mucosal lesions compared to the raw oil.Conclusion: These results indicated great potential for future application of optimized PSO-NLCs formula for antiulcer effect in non-steroidal anti-inflammatory drug (NSAID)-induced gastric ulcer.Keywords: natural products, gastric ulcer, pumpkin seed oil, nano-lipid carriers, optimization, Box–Behnken experimental designAhmed OAAFahmy UABakhaidar REl-Moselhy MAAlfaleh MAAhmed ASFHammad ASAAldawsari HAlhakamy NADove Medical Pressarticlenatural productsgastric ulcerpumpkin seed oilnano-lipid carriersoptimizationbox-behnken experimental designMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2529-2539 (2020)
institution DOAJ
collection DOAJ
language EN
topic natural products
gastric ulcer
pumpkin seed oil
nano-lipid carriers
optimization
box-behnken experimental design
Medicine (General)
R5-920
spellingShingle natural products
gastric ulcer
pumpkin seed oil
nano-lipid carriers
optimization
box-behnken experimental design
Medicine (General)
R5-920
Ahmed OAA
Fahmy UA
Bakhaidar R
El-Moselhy MA
Alfaleh MA
Ahmed ASF
Hammad ASA
Aldawsari H
Alhakamy NA
Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats
description Osama AA Ahmed,1,2 Usama A Fahmy,1 Rana Bakhaidar,1 Mohamed A El-Moselhy,3,4 Mohamed A Alfaleh,5 Al-Shaimaa F Ahmed,4 Asmaa SA Hammad,4 Hibah Aldawsari,1 Nabil A Alhakamy1,6–8 1Advanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 3Department of Pharmacology, School of Pharmacy, Ibn Sina National College, Jeddah 22413, Saudi Arabia; 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 5Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 6Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 7Center of Excellence for Drug Research and Pharmaceutical Industries, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 8King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCorrespondence: Osama AA AhmedAdvanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaTel +966 599120686Email oaahmed@kau.edu.saBackground: Peptic ulcer disease, a painful lesion of the gastric mucosa, is considered one of the most common gastrointestinal disorders. This study aims to investigate the formulation of pumpkin seed oil (PSO)-based nanostructured lipid carriers (NLCs) to utilize PSO as the liquid lipid component of NLCs and to achieve oil dispersion in the nano-range in the stomach.Methods: Box–Behnken design was utilized to deduce the optimum formula with minimum particle size. The optimized PSO-NLCs formula was investigated for gastric ulcer protective effects in Wistar rats by evaluating ulcer index and determination of gastric mucosa oxidative stress parameters.Results: PSO was successfully incorporated as the liquid lipid (LL) component of NLCs. The prepared optimum PSO-NLCs formula showed a size of 64.3 nm. Pretreatment of animals using the optimized PSO-NLCs formula showed significantly (p< 0.001) lower ulcer index compared to indomethacin alone group and significantly (p< 0.05) less mucosal lesions compared to the raw oil.Conclusion: These results indicated great potential for future application of optimized PSO-NLCs formula for antiulcer effect in non-steroidal anti-inflammatory drug (NSAID)-induced gastric ulcer.Keywords: natural products, gastric ulcer, pumpkin seed oil, nano-lipid carriers, optimization, Box–Behnken experimental design
format article
author Ahmed OAA
Fahmy UA
Bakhaidar R
El-Moselhy MA
Alfaleh MA
Ahmed ASF
Hammad ASA
Aldawsari H
Alhakamy NA
author_facet Ahmed OAA
Fahmy UA
Bakhaidar R
El-Moselhy MA
Alfaleh MA
Ahmed ASF
Hammad ASA
Aldawsari H
Alhakamy NA
author_sort Ahmed OAA
title Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats
title_short Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats
title_full Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats
title_fullStr Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats
title_full_unstemmed Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats
title_sort pumpkin oil–based nanostructured lipid carrier system for antiulcer effect in nsaid-induced gastric ulcer model in rats
publisher Dove Medical Press
publishDate 2020
url https://doaj.org/article/c86166e567b4474dbe5eed68355a7906
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