PR-LncRNA signature regulates glioma cell activity through expression of SOX factors

Abstract Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs...

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Autores principales: Sergio Torres-Bayona, Paula Aldaz, Jaione Auzmendi-Iriarte, Ander Saenz-Antoñanzas, Idoia Garcia, Mariano Arrazola, Daniela Gerovska, Jose Undabeitia, Arrate Querejeta, Larraitz Egaña, Jorge Villanúa, Irune Ruiz, Cristina Sarasqueta, Enrique Urculo, Marcos J. Araúzo-Bravo, Maite Huarte, Nicolas Samprón, Ander Matheu
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Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/c872a6ab60e84e74817e34215cc393e2
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spelling oai:doaj.org-article:c872a6ab60e84e74817e34215cc393e22021-12-02T11:40:47ZPR-LncRNA signature regulates glioma cell activity through expression of SOX factors10.1038/s41598-018-30836-52045-2322https://doaj.org/article/c872a6ab60e84e74817e34215cc393e22018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30836-5https://doaj.org/toc/2045-2322Abstract Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRNAs) in colorectal cancer. Our aim was to identify the expression and function of this signature in gliomas. We found that the expression of the four PR-LncRNAs tested was high in human low-grade glioma samples and diminished with increasing grade of disease, being the lowest in glioblastoma samples. Functional assays demonstrated that PR-LncRNA silencing increased glioma cell proliferation and oncosphere formation. Mechanistically, we found an inverse correlation between PR-LncRNA expression and SOX1, SOX2 and SOX9 stem cell factors in human glioma biopsies and in glioma cells in vitro. Moreover, knock-down of SOX activity abolished the effect of PR-LncRNA silencing in glioma cell activity. In conclusion, our results demonstrate that the expression and function of PR-LncRNAs are significantly altered in gliomagenesis and that their activity is mediated by SOX factors. These results may provide important insights into the mechanisms responsible for glioblastoma pathogenesis.Sergio Torres-BayonaPaula AldazJaione Auzmendi-IriarteAnder Saenz-AntoñanzasIdoia GarciaMariano ArrazolaDaniela GerovskaJose UndabeitiaArrate QuerejetaLarraitz EgañaJorge VillanúaIrune RuizCristina SarasquetaEnrique UrculoMarcos J. Araúzo-BravoMaite HuarteNicolas SamprónAnder MatheuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Sergio Torres-Bayona
Paula Aldaz
Jaione Auzmendi-Iriarte
Ander Saenz-Antoñanzas
Idoia Garcia
Mariano Arrazola
Daniela Gerovska
Jose Undabeitia
Arrate Querejeta
Larraitz Egaña
Jorge Villanúa
Irune Ruiz
Cristina Sarasqueta
Enrique Urculo
Marcos J. Araúzo-Bravo
Maite Huarte
Nicolas Samprón
Ander Matheu
PR-LncRNA signature regulates glioma cell activity through expression of SOX factors
description Abstract Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRNAs) in colorectal cancer. Our aim was to identify the expression and function of this signature in gliomas. We found that the expression of the four PR-LncRNAs tested was high in human low-grade glioma samples and diminished with increasing grade of disease, being the lowest in glioblastoma samples. Functional assays demonstrated that PR-LncRNA silencing increased glioma cell proliferation and oncosphere formation. Mechanistically, we found an inverse correlation between PR-LncRNA expression and SOX1, SOX2 and SOX9 stem cell factors in human glioma biopsies and in glioma cells in vitro. Moreover, knock-down of SOX activity abolished the effect of PR-LncRNA silencing in glioma cell activity. In conclusion, our results demonstrate that the expression and function of PR-LncRNAs are significantly altered in gliomagenesis and that their activity is mediated by SOX factors. These results may provide important insights into the mechanisms responsible for glioblastoma pathogenesis.
format article
author Sergio Torres-Bayona
Paula Aldaz
Jaione Auzmendi-Iriarte
Ander Saenz-Antoñanzas
Idoia Garcia
Mariano Arrazola
Daniela Gerovska
Jose Undabeitia
Arrate Querejeta
Larraitz Egaña
Jorge Villanúa
Irune Ruiz
Cristina Sarasqueta
Enrique Urculo
Marcos J. Araúzo-Bravo
Maite Huarte
Nicolas Samprón
Ander Matheu
author_facet Sergio Torres-Bayona
Paula Aldaz
Jaione Auzmendi-Iriarte
Ander Saenz-Antoñanzas
Idoia Garcia
Mariano Arrazola
Daniela Gerovska
Jose Undabeitia
Arrate Querejeta
Larraitz Egaña
Jorge Villanúa
Irune Ruiz
Cristina Sarasqueta
Enrique Urculo
Marcos J. Araúzo-Bravo
Maite Huarte
Nicolas Samprón
Ander Matheu
author_sort Sergio Torres-Bayona
title PR-LncRNA signature regulates glioma cell activity through expression of SOX factors
title_short PR-LncRNA signature regulates glioma cell activity through expression of SOX factors
title_full PR-LncRNA signature regulates glioma cell activity through expression of SOX factors
title_fullStr PR-LncRNA signature regulates glioma cell activity through expression of SOX factors
title_full_unstemmed PR-LncRNA signature regulates glioma cell activity through expression of SOX factors
title_sort pr-lncrna signature regulates glioma cell activity through expression of sox factors
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/c872a6ab60e84e74817e34215cc393e2
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